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This early report singled out intestinal and vaginal lactobacilli for a potent ability to produce tryptophan metabolites that activate host-AHRs in epithelium tissue. The activation of epithelial AHRs induces the production of a myriad of antimicrobial compounds and was found to be essential for control of opportunistic pathogens. Importantly, AHR knockout mice infected with Candida albicans could not be rescued by introduction of tryptophan-metabolizing lactobacilli.
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In TLR1 knockout mice the acute intestinal infection of Yersinia enterocolitica destabilizes mucosal immunity allowing blooms of δ-Proteobacteria and diminished levels of other taxa, including lactobacilli. Chronic immune responses against the commensal microbiota were observed two months postinfection. Supplementation with L. reuteri was sufficient to revert the immune system towards homeostasis.
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Human single nucleotide polymorphism (SNP) studies indicate caspase recruitment domain family member 9 (CARD9) alleles are associated with inflammatory bowel disease and that this risk allele may influence the development of a pro-inflammatory microbiome. By using CARD9 knockout mice, this report links CARD9 deficiencies to an altered intestinal microbiome with reduced production of AHR ligands. L. reuteri was identified as a significantly depleted taxon and replenishment of this species improved symptoms of colitis in CARD9 knockout mice.
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Lamas, B., Richard, M.L., Leducq, V., Pham, H-P., Michel, M-L., Da Costa, G., Bridonneau, C., Jegou, S., Hoffmann, T.W., Natividad, J.M., et al. CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands. Nat Med 22 (2016), 598–605 Human single nucleotide polymorphism (SNP) studies indicate caspase recruitment domain family member 9 (CARD9) alleles are associated with inflammatory bowel disease and that this risk allele may influence the development of a pro-inflammatory microbiome. By using CARD9 knockout mice, this report links CARD9 deficiencies to an altered intestinal microbiome with reduced production of AHR ligands. L. reuteri was identified as a significantly depleted taxon and replenishment of this species improved symptoms of colitis in CARD9 knockout mice.
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This human study utilizing metagenomic sequencing on the fecal microbiota identified several species of intestinal Lactobacillus species that were elevated in the oral and fecal microbiota of RA patients compared with healthy subjects.
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Zhang, X., Zhang, D., Jia, H., Feng, Q., Wang, D., Liang, D., Wu, X., Li, J., Tang, L., Li, Y., et al. The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment. Nat Med 21 (2015), 895–905 This human study utilizing metagenomic sequencing on the fecal microbiota identified several species of intestinal Lactobacillus species that were elevated in the oral and fecal microbiota of RA patients compared with healthy subjects.
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