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Volumn 45, Issue 11, 2017, Pages

A platform for functional assessment of large variant libraries in mammalian cells

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; AMPLICON; ARTICLE; CELL ENGINEERING; CLONE; CONTROLLED STUDY; DNA TEMPLATE; EMBRYO; GENE AMPLIFICATION; GENE CASSETTE; GENE EXPRESSION; GENE EXPRESSION ASSAY; GENE FREQUENCY; GENE INSERTION; GENE LIBRARY; GENE LOCUS; GENE SEQUENCE; GENETIC RECOMBINATION; GENETIC TRANSFECTION; GENETIC VARIABILITY; GENOTYPE; GENOTYPE PHENOTYPE CORRELATION; HEK293T CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN STABILITY; RECOMBINANT PLASMID; STOP CODON; TRANSGENE; TRANSGENICS; BIOSYNTHESIS; DEPENDOPARVOVIRUS; GENETIC ASSOCIATION STUDY; GENETIC VARIATION; GENETICS; HEK293 CELL LINE; NUCLEOTIDE SEQUENCE;

EID: 85027278412     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkx183     Document Type: Article
Times cited : (95)

References (39)
  • 1
    • 84988664660 scopus 로고    scopus 로고
    • The power of multiplexed functional analysis of genetic variants
    • Gasperini,M., Starita,L. and Shendure,J. (2016) The power of multiplexed functional analysis of genetic variants. Nat. Protoc., 11, 1782-1787.
    • (2016) Nat. Protoc , vol.11 , pp. 1782-1787
    • Gasperini, M.1    Starita, L.2    Shendure, J.3
  • 3
    • 84892749369 scopus 로고    scopus 로고
    • Genetic screens in human cells using the CRISPR-Cas9 system
    • Wang,T., Wei,J.J., Sabatini,D.M. and Lander,E.S. (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science, 343, 80-84.
    • (2014) Science , vol.343 , pp. 80-84
    • Wang, T.1    Wei, J.J.2    Sabatini, D.M.3    Lander, E.S.4
  • 4
    • 84905217368 scopus 로고    scopus 로고
    • Deep mutational scanning: A new style of protein science
    • Fowler,D.M. and Fields,S. (2014) Deep mutational scanning: a new style of protein science. Nat. Methods, 11, 801-807.
    • (2014) Nat. Methods , vol.11 , pp. 801-807
    • Fowler, D.M.1    Fields, S.2
  • 5
    • 84907338120 scopus 로고    scopus 로고
    • Quantitative analysis of mammalian translation initiation sites by FACS-seq
    • Noderer,W.L. and Flockhart,R.J. (2014) Quantitative analysis of mammalian translation initiation sites by FACS-seq. Mol. Syst. Biol., 10, 748.
    • (2014) Mol. Syst. Biol , vol.10 , pp. 748
    • Noderer, W.L.1    Flockhart, R.J.2
  • 7
    • 77954573744 scopus 로고    scopus 로고
    • Accurately measuring recombination between closely related HIV-1 genomes
    • Schlub,T.E., Smyth,R.P., Grimm,A.J., Mak,J. and Davenport,M.P. (2010) Accurately measuring recombination between closely related HIV-1 genomes. PLoS Comput. Biol., 6, e1000766.
    • (2010) PLoS Comput. Biol , vol.6 , pp. e1000766
    • Schlub, T.E.1    Smyth, R.P.2    Grimm, A.J.3    Mak, J.4    Davenport, M.P.5
  • 10
    • 84907257107 scopus 로고    scopus 로고
    • Saturation editing of genomic regions by multiplex homology-directed repair
    • Findlay,G.M., Boyle,E.A., Hause,R.J., Klein,J.C. and Shendure,J. (2014) Saturation editing of genomic regions by multiplex homology-directed repair. Nature, 513, 120-123.
    • (2014) Nature , vol.513 , pp. 120-123
    • Findlay, G.M.1    Boyle, E.A.2    Hause, R.J.3    Klein, J.C.4    Shendure, J.5
  • 11
    • 0034783643 scopus 로고    scopus 로고
    • Systematic determination of the packaging limit of lentiviral vectors
    • Kumar,M., Keller,B., Makalou,N. and Sutton,R.E. (2001) Systematic determination of the packaging limit of lentiviral vectors. Hum. Gene Ther., 12, 1893-905.
    • (2001) Hum. Gene Ther , vol.12 , pp. 1893-1905
    • Kumar, M.1    Keller, B.2    Makalou, N.3    Sutton, R.E.4
  • 12
    • 84969963103 scopus 로고    scopus 로고
    • Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
    • He,X., Tan,C., Wang,F., Wang,Y., Zhou,R., Cui,D., You,W., Zhao,H., Ren,J., Feng,B. et al. (2016) Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res., 44, e85.
    • (2016) Nucleic Acids Res , vol.44 , pp. e85
    • He, X.1    Tan, C.2    Wang, F.3    Wang, Y.4    Zhou, R.5    Cui, D.6    You, W.7    Zhao, H.8    Ren, J.9    Feng, B.10
  • 13
    • 0348013054 scopus 로고    scopus 로고
    • Toward a functional annotation of the human genome using artificial transcription factors
    • Lee,D., Park,J.W., Kim,Y., Kim,J., Lee,Y., Kim,J. and Kim,J. (2003) Toward a functional annotation of the human genome using artificial transcription factors. Genome Res., 13, 2708-2716.
    • (2003) Genome Res , vol.13 , pp. 2708-2716
    • Lee, D.1    Park, J.W.2    Kim, Y.3    Kim, J.4    Lee, Y.5    Kim, J.6    Kim, J.7
  • 14
    • 84885506325 scopus 로고    scopus 로고
    • Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome
    • Xu,Z., Thomas,L., Davies,B., Chalmers,R., Smith,M. and Brown,W. (2013) Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome. BMC Biotechnol., 13, 87.
    • (2013) BMC Biotechnol , vol.13 , pp. 87
    • Xu, Z.1    Thomas, L.2    Davies, B.3    Chalmers, R.4    Smith, M.5    Brown, W.6
  • 15
    • 79952085508 scopus 로고    scopus 로고
    • A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector
    • Yamaguchi,S., Kazuki,Y., Nakayama,Y., Nanba,E., Oshimura,M. and Ohbayashi,T. (2011) A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. PLoS One, 6, e17267.
    • (2011) PLoS One , vol.6 , pp. e17267
    • Yamaguchi, S.1    Kazuki, Y.2    Nakayama, Y.3    Nanba, E.4    Oshimura, M.5    Ohbayashi, T.6
  • 16
    • 84893433412 scopus 로고    scopus 로고
    • The ins and outs of serine integrase site-specific recombination
    • Rutherford,K. and Van Duyne,G.D. (2014) The ins and outs of serine integrase site-specific recombination. Curr. Opin. Struct. Biol., 24, 125-131.
    • (2014) Curr. Opin. Struct. Biol , vol.24 , pp. 125-131
    • Rutherford, K.1    Van Duyne, G.D.2
  • 17
    • 33646843556 scopus 로고    scopus 로고
    • Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells
    • Russell,J.P., Chang,D.W., Tretiakova,A. and Padidam,M. (2006) Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells. Biotechniques, 40, 460-464.
    • (2006) Biotechniques , vol.40 , pp. 460-464
    • Russell, J.P.1    Chang, D.W.2    Tretiakova, A.3    Padidam, M.4
  • 22
    • 62849113646 scopus 로고    scopus 로고
    • Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection
    • Cohen,R.N., van der Aa,M.A., Macaraeg,N., Lee,A.P. and Szoka,F.C. (2009) Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection. J. Control. Release, 135, 166-174.
    • (2009) J. Control. Release , vol.135 , pp. 166-174
    • Cohen, R.N.1    Van Der Aa, M.A.2    Macaraeg, N.3    Lee, A.P.4    Szoka, F.C.5
  • 23
    • 84983748545 scopus 로고    scopus 로고
    • Sources of PCR-induced distortions in high-throughput sequencing data sets
    • Kebschull,J.M. and Zador,A.M. (2015) Sources of PCR-induced distortions in high-throughput sequencing data sets. Nucleic Acids Res., 43, 1-15.
    • (2015) Nucleic Acids Res , vol.43 , pp. 1-15
    • Kebschull, J.M.1    Zador, A.M.2
  • 24
    • 79960683356 scopus 로고    scopus 로고
    • The N-end rule pathway and regulation by proteolysis
    • Varshavsky,A. (2011) The N-end rule pathway and regulation by proteolysis. Protein Sci., 20, 1298-1345.
    • (2011) Protein Sci , vol.20 , pp. 1298-1345
    • Varshavsky, A.1
  • 25
    • 84908478523 scopus 로고    scopus 로고
    • The eukaryotic N-end rule pathway: Conserved mechanisms and diverse functions
    • Gibbs,D.J., Bacardit,J., Bachmair,A. and Holdsworth,M.J. (2014) The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions. Trends Cell Biol., 24, 603-611.
    • (2014) Trends Cell Biol , vol.24 , pp. 603-611
    • Gibbs, D.J.1    Bacardit, J.2    Bachmair, A.3    Holdsworth, M.J.4
  • 27
    • 28944437762 scopus 로고    scopus 로고
    • Ubiquitin fusion technique and related methods
    • Varshavsky,A. (2005) Ubiquitin fusion technique and related methods. Methods Enzymol., 399, 777-799.
    • (2005) Methods Enzymol , vol.399 , pp. 777-799
    • Varshavsky, A.1
  • 29
    • 84878188440 scopus 로고    scopus 로고
    • Chromatin insulators: Linking genome organization to cellular function
    • Phillips-Cremins,J. and Corces,V. (2013) Chromatin insulators: linking genome organization to cellular function. Mol. Cell, 50, 461-474.
    • (2013) Mol. Cell , vol.50 , pp. 461-474
    • Phillips-Cremins, J.1    Corces, V.2
  • 30
    • 13844292517 scopus 로고    scopus 로고
    • Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo
    • Chen,Z.-Y., He,C.-Y. and Kay,M.A (2005) Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo. Hum. Gene Ther., 16, 126-131.
    • (2005) Hum. Gene Ther , vol.16 , pp. 126-131
    • Chen, Z.-Y.1    He, C.-Y.2    Kay, M.A.3
  • 32
    • 84924769665 scopus 로고    scopus 로고
    • Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway
    • Park,S.-E., Kim,J.-M., Seok,O.-H., Cho,H., Wadas,B., Kim,S.-Y., Varshavsky,A. and Hwang,C.-S. (2015) Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science, 347, 1249-1252.
    • (2015) Science , vol.347 , pp. 1249-1252
    • Park, S.-E.1    Kim, J.-M.2    Seok, O.-H.3    Cho, H.4    Wadas, B.5    Kim, S.-Y.6    Varshavsky, A.7    Hwang, C.-S.8
  • 33
    • 77957790301 scopus 로고    scopus 로고
    • Structural basis of substrate recognition and specificity in the N-end rule pathway
    • Matta-Camacho,E., Kozlov,G., Li,F.F. and Gehring,K. (2010) Structural basis of substrate recognition and specificity in the N-end rule pathway. Nat. Struct. Mol. Biol., 17, 1182-1187.
    • (2010) Nat. Struct. Mol. Biol , vol.17 , pp. 1182-1187
    • Matta-Camacho, E.1    Kozlov, G.2    Li, F.F.3    Gehring, K.4
  • 36
    • 85007564263 scopus 로고    scopus 로고
    • High-throughput discovery of post-transcriptional cis-regulatory elements
    • Wissink,E.M., Fogarty,E.A. and Grimson,A. (2016) High-throughput discovery of post-transcriptional cis-regulatory elements. BMC Genomics, 17, 177.
    • (2016) BMC Genomics , vol.17 , pp. 177
    • Wissink, E.M.1    Fogarty, E.A.2    Grimson, A.3
  • 37
    • 84907022693 scopus 로고    scopus 로고
    • Measuring the activity of protein variants on a large scale using deep mutational scanning
    • Fowler,D.M., Stephany,J.J. and Fields,S. (2014) Measuring the activity of protein variants on a large scale using deep mutational scanning. Nat. Protoc., 9, 2267-2284.
    • (2014) Nat. Protoc , vol.9 , pp. 2267-2284
    • Fowler, D.M.1    Stephany, J.J.2    Fields, S.3
  • 38
    • 84960091824 scopus 로고    scopus 로고
    • Sort-seq under the hood: Implications of design choices on large-scale characterization of sequence-function relations
    • Peterman,N. and Levine,E. (2016) Sort-seq under the hood: implications of design choices on large-scale characterization of sequence-function relations. BMC Genomics, 17, 206.
    • (2016) BMC Genomics , vol.17 , pp. 206
    • Peterman, N.1    Levine, E.2


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