-
1
-
-
84988664660
-
The power of multiplexed functional analysis of genetic variants
-
Gasperini,M., Starita,L. and Shendure,J. (2016) The power of multiplexed functional analysis of genetic variants. Nat. Protoc., 11, 1782-1787.
-
(2016)
Nat. Protoc
, vol.11
, pp. 1782-1787
-
-
Gasperini, M.1
Starita, L.2
Shendure, J.3
-
2
-
-
84892765883
-
Genome-scale CRISPR-cas9 knockout
-
Shalem,O., Sanjana,E.N., Hartenian,E. and Zhang,F. (2014) Genome-Scale CRISPR-Cas9 Knockout. Science, 343, 84-88.
-
(2014)
Science
, vol.343
, pp. 84-88
-
-
Shalem, O.1
Sanjana, E.N.2
Hartenian, E.3
Zhang, F.4
-
3
-
-
84892749369
-
Genetic screens in human cells using the CRISPR-Cas9 system
-
Wang,T., Wei,J.J., Sabatini,D.M. and Lander,E.S. (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science, 343, 80-84.
-
(2014)
Science
, vol.343
, pp. 80-84
-
-
Wang, T.1
Wei, J.J.2
Sabatini, D.M.3
Lander, E.S.4
-
4
-
-
84905217368
-
Deep mutational scanning: A new style of protein science
-
Fowler,D.M. and Fields,S. (2014) Deep mutational scanning: a new style of protein science. Nat. Methods, 11, 801-807.
-
(2014)
Nat. Methods
, vol.11
, pp. 801-807
-
-
Fowler, D.M.1
Fields, S.2
-
5
-
-
84907338120
-
Quantitative analysis of mammalian translation initiation sites by FACS-seq
-
Noderer,W.L. and Flockhart,R.J. (2014) Quantitative analysis of mammalian translation initiation sites by FACS-seq. Mol. Syst. Biol., 10, 748.
-
(2014)
Mol. Syst. Biol
, vol.10
, pp. 748
-
-
Noderer, W.L.1
Flockhart, R.J.2
-
6
-
-
84941105575
-
A platform for rapid prototyping of synthetic gene networks in mammalian cells
-
Duportet,X., Wroblewska,L., Guye,P., Li,Y., Eyquem,J., Rieders,J., Rimchala,T., Batt,G. and Weiss,R. (2014) A platform for rapid prototyping of synthetic gene networks in mammalian cells. Nucleic Acids Res., 42, 13440-13451.
-
(2014)
Nucleic Acids Res
, vol.42
, pp. 13440-13451
-
-
Duportet, X.1
Wroblewska, L.2
Guye, P.3
Li, Y.4
Eyquem, J.5
Rieders, J.6
Rimchala, T.7
Batt, G.8
Weiss, R.9
-
7
-
-
77954573744
-
Accurately measuring recombination between closely related HIV-1 genomes
-
Schlub,T.E., Smyth,R.P., Grimm,A.J., Mak,J. and Davenport,M.P. (2010) Accurately measuring recombination between closely related HIV-1 genomes. PLoS Comput. Biol., 6, e1000766.
-
(2010)
PLoS Comput. Biol
, vol.6
, pp. e1000766
-
-
Schlub, T.E.1
Smyth, R.P.2
Grimm, A.J.3
Mak, J.4
Davenport, M.P.5
-
8
-
-
84994803156
-
Phenotypic characterization of a comprehensive set of MAPK1/ERK2 missense mutants
-
Brenan,L., Andreev,A., Cohen,O., Pantel,S., Kamburov,A., Cacchiarelli,D., Persky,N.S., Zhu,C., Bagul,M., Goetz,E.M. et al. (2016) Phenotypic characterization of a comprehensive set of MAPK1/ERK2 missense mutants. Cell Rep., 17, 1171-1183.
-
(2016)
Cell Rep
, vol.17
, pp. 1171-1183
-
-
Brenan, L.1
Andreev, A.2
Cohen, O.3
Pantel, S.4
Kamburov, A.5
Cacchiarelli, D.6
Persky, N.S.7
Zhu, C.8
Bagul, M.9
Goetz, E.M.10
-
9
-
-
84991585415
-
Prospective functional classification of all possible missense variants in PPARG
-
Majithia,A.R., Tsuda,B., Agostini,M., Gnanapradeepan,K., Rice,R., Peloso,G., Patel,K.A., Zhang,X., Broekema,M.F., Patterson,N. et al. (2016) Prospective functional classification of all possible missense variants in PPARG. Nat. Genet., 48, 1570-1575.
-
(2016)
Nat. Genet
, vol.48
, pp. 1570-1575
-
-
Majithia, A.R.1
Tsuda, B.2
Agostini, M.3
Gnanapradeepan, K.4
Rice, R.5
Peloso, G.6
Patel, K.A.7
Zhang, X.8
Broekema, M.F.9
Patterson, N.10
-
10
-
-
84907257107
-
Saturation editing of genomic regions by multiplex homology-directed repair
-
Findlay,G.M., Boyle,E.A., Hause,R.J., Klein,J.C. and Shendure,J. (2014) Saturation editing of genomic regions by multiplex homology-directed repair. Nature, 513, 120-123.
-
(2014)
Nature
, vol.513
, pp. 120-123
-
-
Findlay, G.M.1
Boyle, E.A.2
Hause, R.J.3
Klein, J.C.4
Shendure, J.5
-
11
-
-
0034783643
-
Systematic determination of the packaging limit of lentiviral vectors
-
Kumar,M., Keller,B., Makalou,N. and Sutton,R.E. (2001) Systematic determination of the packaging limit of lentiviral vectors. Hum. Gene Ther., 12, 1893-905.
-
(2001)
Hum. Gene Ther
, vol.12
, pp. 1893-1905
-
-
Kumar, M.1
Keller, B.2
Makalou, N.3
Sutton, R.E.4
-
12
-
-
84969963103
-
Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
-
He,X., Tan,C., Wang,F., Wang,Y., Zhou,R., Cui,D., You,W., Zhao,H., Ren,J., Feng,B. et al. (2016) Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res., 44, e85.
-
(2016)
Nucleic Acids Res
, vol.44
, pp. e85
-
-
He, X.1
Tan, C.2
Wang, F.3
Wang, Y.4
Zhou, R.5
Cui, D.6
You, W.7
Zhao, H.8
Ren, J.9
Feng, B.10
-
13
-
-
0348013054
-
Toward a functional annotation of the human genome using artificial transcription factors
-
Lee,D., Park,J.W., Kim,Y., Kim,J., Lee,Y., Kim,J. and Kim,J. (2003) Toward a functional annotation of the human genome using artificial transcription factors. Genome Res., 13, 2708-2716.
-
(2003)
Genome Res
, vol.13
, pp. 2708-2716
-
-
Lee, D.1
Park, J.W.2
Kim, Y.3
Kim, J.4
Lee, Y.5
Kim, J.6
Kim, J.7
-
14
-
-
84885506325
-
Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome
-
Xu,Z., Thomas,L., Davies,B., Chalmers,R., Smith,M. and Brown,W. (2013) Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome. BMC Biotechnol., 13, 87.
-
(2013)
BMC Biotechnol
, vol.13
, pp. 87
-
-
Xu, Z.1
Thomas, L.2
Davies, B.3
Chalmers, R.4
Smith, M.5
Brown, W.6
-
15
-
-
79952085508
-
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector
-
Yamaguchi,S., Kazuki,Y., Nakayama,Y., Nanba,E., Oshimura,M. and Ohbayashi,T. (2011) A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. PLoS One, 6, e17267.
-
(2011)
PLoS One
, vol.6
, pp. e17267
-
-
Yamaguchi, S.1
Kazuki, Y.2
Nakayama, Y.3
Nanba, E.4
Oshimura, M.5
Ohbayashi, T.6
-
16
-
-
84893433412
-
The ins and outs of serine integrase site-specific recombination
-
Rutherford,K. and Van Duyne,G.D. (2014) The ins and outs of serine integrase site-specific recombination. Curr. Opin. Struct. Biol., 24, 125-131.
-
(2014)
Curr. Opin. Struct. Biol
, vol.24
, pp. 125-131
-
-
Rutherford, K.1
Van Duyne, G.D.2
-
17
-
-
33646843556
-
Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells
-
Russell,J.P., Chang,D.W., Tretiakova,A. and Padidam,M. (2006) Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells. Biotechniques, 40, 460-464.
-
(2006)
Biotechniques
, vol.40
, pp. 460-464
-
-
Russell, J.P.1
Chang, D.W.2
Tretiakova, A.3
Padidam, M.4
-
18
-
-
84898990861
-
DICE, an efficient system for iterative genomic editing in human pluripotent stem cells
-
Zhu,F., Gamboa,M., Farruggio,A.P., Hippenmeyer,S., Tasic,B., Schule,B., Chen-Tsai,Y. and Calos,M.P. (2014) DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Res., 42, e34.
-
(2014)
Nucleic Acids Res
, vol.42
, pp. e34
-
-
Zhu, F.1
Gamboa, M.2
Farruggio, A.P.3
Hippenmeyer, S.4
Tasic, B.5
Schule, B.6
Chen-Tsai, Y.7
Calos, M.P.8
-
19
-
-
84948947590
-
A modular open platform for systematic functional studies under physiological conditions
-
Mulholland,C.B., Smets,M., Schmidtmann,E., Leidescher,S., Markaki,Y., Hofweber,M., Qin,W., Manzo,M., Kremmer,E., Thanisch,K. et al. (2015) A modular open platform for systematic functional studies under physiological conditions. Nucleic Acids Res., 43, e112.
-
(2015)
Nucleic Acids Res
, vol.43
, pp. e112
-
-
Mulholland, C.B.1
Smets, M.2
Schmidtmann, E.3
Leidescher, S.4
Markaki, Y.5
Hofweber, M.6
Qin, W.7
Manzo, M.8
Kremmer, E.9
Thanisch, K.10
-
20
-
-
67349270900
-
Enzymatic assembly of DNA molecules up to several hundred kilobases
-
III C.A.H.
-
Gibson,D.G., Young,L., Chuang,R.-Y., Venter,J.C., Hutchison,C.A., Smith,H.O., Iii,C.A.H. and America,N. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods, 6, 343-345.
-
(2009)
Nat. Methods
, vol.6
, pp. 343-345
-
-
Gibson, D.G.1
Young, L.2
Chuang, R.-Y.3
Venter, J.C.4
Hutchison, C.A.5
Smith, H.O.6
America, N.7
-
21
-
-
84907364884
-
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations
-
Lin,Y.-C., Boone,M., Meuris,L., Lemmens,I., Van Roy,N., Soete,A., Reumers,J., Moisse,M., Plaisance,S., Drmanac,R. et al. (2014) Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Nat. Commun., 5, 4767.
-
(2014)
Nat. Commun
, vol.5
, pp. 4767
-
-
Lin, Y.-C.1
Boone, M.2
Meuris, L.3
Lemmens, I.4
Van Roy, N.5
Soete, A.6
Reumers, J.7
Moisse, M.8
Plaisance, S.9
Drmanac, R.10
-
22
-
-
62849113646
-
Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection
-
Cohen,R.N., van der Aa,M.A., Macaraeg,N., Lee,A.P. and Szoka,F.C. (2009) Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection. J. Control. Release, 135, 166-174.
-
(2009)
J. Control. Release
, vol.135
, pp. 166-174
-
-
Cohen, R.N.1
Van Der Aa, M.A.2
Macaraeg, N.3
Lee, A.P.4
Szoka, F.C.5
-
23
-
-
84983748545
-
Sources of PCR-induced distortions in high-throughput sequencing data sets
-
Kebschull,J.M. and Zador,A.M. (2015) Sources of PCR-induced distortions in high-throughput sequencing data sets. Nucleic Acids Res., 43, 1-15.
-
(2015)
Nucleic Acids Res
, vol.43
, pp. 1-15
-
-
Kebschull, J.M.1
Zador, A.M.2
-
24
-
-
79960683356
-
The N-end rule pathway and regulation by proteolysis
-
Varshavsky,A. (2011) The N-end rule pathway and regulation by proteolysis. Protein Sci., 20, 1298-1345.
-
(2011)
Protein Sci
, vol.20
, pp. 1298-1345
-
-
Varshavsky, A.1
-
25
-
-
84908478523
-
The eukaryotic N-end rule pathway: Conserved mechanisms and diverse functions
-
Gibbs,D.J., Bacardit,J., Bachmair,A. and Holdsworth,M.J. (2014) The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions. Trends Cell Biol., 24, 603-611.
-
(2014)
Trends Cell Biol
, vol.24
, pp. 603-611
-
-
Gibbs, D.J.1
Bacardit, J.2
Bachmair, A.3
Holdsworth, M.J.4
-
26
-
-
0024474145
-
Universality and structure of the N-end rule
-
Gonda,D.K., Bachmair,a, W unning,I., Tobias,J.W., Lane,W.S. and Varshavsky,A. (1989) Universality and structure of the N-end rule. J. Biol. Chem., 264, 16700-16712.
-
(1989)
J. Biol. Chem
, vol.264
, pp. 16700-16712
-
-
Gonda, D.K.1
Bachmair Unning Wa, I.2
Tobias, J.W.3
Lane, W.S.4
Varshavsky, A.5
-
27
-
-
28944437762
-
Ubiquitin fusion technique and related methods
-
Varshavsky,A. (2005) Ubiquitin fusion technique and related methods. Methods Enzymol., 399, 777-799.
-
(2005)
Methods Enzymol
, vol.399
, pp. 777-799
-
-
Varshavsky, A.1
-
28
-
-
70249114821
-
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases
-
Hockemeyer,D., Soldner,F., Beard,C., Gao,Q., Mitalipova,M., DeKelver,R.C., Katibah,G.E., Amora,R., Boydston,E.A., Zeitler,B. et al. (2009) Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol., 27, 851-857.
-
(2009)
Nat. Biotechnol
, vol.27
, pp. 851-857
-
-
Hockemeyer, D.1
Soldner, F.2
Beard, C.3
Gao, Q.4
Mitalipova, M.5
DeKelver, R.C.6
Katibah, G.E.7
Amora, R.8
Boydston, E.A.9
Zeitler, B.10
-
29
-
-
84878188440
-
Chromatin insulators: Linking genome organization to cellular function
-
Phillips-Cremins,J. and Corces,V. (2013) Chromatin insulators: linking genome organization to cellular function. Mol. Cell, 50, 461-474.
-
(2013)
Mol. Cell
, vol.50
, pp. 461-474
-
-
Phillips-Cremins, J.1
Corces, V.2
-
30
-
-
13844292517
-
Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo
-
Chen,Z.-Y., He,C.-Y. and Kay,M.A (2005) Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo. Hum. Gene Ther., 16, 126-131.
-
(2005)
Hum. Gene Ther
, vol.16
, pp. 126-131
-
-
Chen, Z.-Y.1
He, C.-Y.2
Kay, M.A.3
-
31
-
-
84856484968
-
Counting absolute numbers of molecules using unique molecular identifiers
-
Kivioja,T., Vaharautio,A., Karlsson,K., Bonke,M., Enge,M., Linnarsson,S. and Taipale,J. (2011) Counting absolute numbers of molecules using unique molecular identifiers. Nat. Methods, 9, 72-74.
-
(2011)
Nat. Methods
, vol.9
, pp. 72-74
-
-
Kivioja, T.1
Vaharautio, A.2
Karlsson, K.3
Bonke, M.4
Enge, M.5
Linnarsson, S.6
Taipale, J.7
-
32
-
-
84924769665
-
Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway
-
Park,S.-E., Kim,J.-M., Seok,O.-H., Cho,H., Wadas,B., Kim,S.-Y., Varshavsky,A. and Hwang,C.-S. (2015) Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science, 347, 1249-1252.
-
(2015)
Science
, vol.347
, pp. 1249-1252
-
-
Park, S.-E.1
Kim, J.-M.2
Seok, O.-H.3
Cho, H.4
Wadas, B.5
Kim, S.-Y.6
Varshavsky, A.7
Hwang, C.-S.8
-
33
-
-
77957790301
-
Structural basis of substrate recognition and specificity in the N-end rule pathway
-
Matta-Camacho,E., Kozlov,G., Li,F.F. and Gehring,K. (2010) Structural basis of substrate recognition and specificity in the N-end rule pathway. Nat. Struct. Mol. Biol., 17, 1182-1187.
-
(2010)
Nat. Struct. Mol. Biol
, vol.17
, pp. 1182-1187
-
-
Matta-Camacho, E.1
Kozlov, G.2
Li, F.F.3
Gehring, K.4
-
34
-
-
59449093066
-
The substrate recognition domains of the N-end rule pathway
-
Tasaki,T., Zakrzewska,A., Dudgeon,D.D., Jiang,Y., Lazo,J.S. and Kwon,Y.T. (2009) The substrate recognition domains of the N-end rule pathway. J. Biol. Chem., 284, 1884-1895.
-
(2009)
J. Biol. Chem
, vol.284
, pp. 1884-1895
-
-
Tasaki, T.1
Zakrzewska, A.2
Dudgeon, D.D.3
Jiang, Y.4
Lazo, J.S.5
Kwon, Y.T.6
-
35
-
-
55049110221
-
The molecular basis of N-end rule recognition
-
Wang,K.H., Roman-Hernandez,G., Grant,R.A., Sauer,R.T. and Baker,T.A. (2008) The molecular basis of N-end rule recognition. Mol. Cell, 32, 406-414.
-
(2008)
Mol. Cell
, vol.32
, pp. 406-414
-
-
Wang, K.H.1
Roman-Hernandez, G.2
Grant, R.A.3
Sauer, R.T.4
Baker, T.A.5
-
36
-
-
85007564263
-
High-throughput discovery of post-transcriptional cis-regulatory elements
-
Wissink,E.M., Fogarty,E.A. and Grimson,A. (2016) High-throughput discovery of post-transcriptional cis-regulatory elements. BMC Genomics, 17, 177.
-
(2016)
BMC Genomics
, vol.17
, pp. 177
-
-
Wissink, E.M.1
Fogarty, E.A.2
Grimson, A.3
-
37
-
-
84907022693
-
Measuring the activity of protein variants on a large scale using deep mutational scanning
-
Fowler,D.M., Stephany,J.J. and Fields,S. (2014) Measuring the activity of protein variants on a large scale using deep mutational scanning. Nat. Protoc., 9, 2267-2284.
-
(2014)
Nat. Protoc
, vol.9
, pp. 2267-2284
-
-
Fowler, D.M.1
Stephany, J.J.2
Fields, S.3
-
38
-
-
84960091824
-
Sort-seq under the hood: Implications of design choices on large-scale characterization of sequence-function relations
-
Peterman,N. and Levine,E. (2016) Sort-seq under the hood: implications of design choices on large-scale characterization of sequence-function relations. BMC Genomics, 17, 206.
-
(2016)
BMC Genomics
, vol.17
, pp. 206
-
-
Peterman, N.1
Levine, E.2
-
39
-
-
84926033357
-
High-resolution sequence-function mapping of full-length proteins
-
Kowalsky,C.A., Klesmith,J.R., Stapleton,J.A., Kelly,V., Reichkitzer,N. and Whitehead,T.A. (2015) High-resolution sequence-function mapping of full-length proteins. PLoS One, 10, e0118193.
-
(2015)
PLoS One
, vol.10
, pp. e0118193
-
-
Kowalsky, C.A.1
Klesmith, J.R.2
Stapleton, J.A.3
Kelly, V.4
Reichkitzer, N.5
Whitehead, T.A.6
|