The first crystal structure of a family 129 glycoside hydrolase from a probiotic bacterium reveals critical residues and metal cofactors

Journal of Biological Chemistry

Volumn 292, Issue 29, 2017, Pages 12126-12138

  Sato, Mayo ^a   Liebschner, Dorothee ^b,e   Yamada, Yusuke ^b   Matsugaki, Naohiro ^b   Arakawa, Takatoshi ^a   Wills, Siobhán S ^c   Hattie, Mitchell ^c   Stubbs, Keith A ^c   Ito, Tasuku ^a,f   Senda, Toshiya ^b   Ashida, Hisashi ^d   Fushinobu, Shinya ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b INSTITUTE OF MATERIALS STRUCTURE SCIENCE   (Japan)

^c UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^d KINKI UNIVERSITY   (Japan)

^e LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^f Yamada Apiculture Center Inc   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CATALYSIS; GLYCOSIDES; HYDROGEN BONDS; HYDROLASES; METAL IONS; METALS; PROBIOTICS; QUANTUM CHEMISTRY; SUGARS;

BIFIDOBACTERIUM BIFIDUM; CATALYTIC MECHANISMS; GASTROINTESTINAL TRACT; SINGLE-WAVELENGTH ANOMALOUS DISPERSIONS; STRUCTURAL INFORMATION; STRUCTURAL SIMILARITY; SUBSTRATE RECOGNITION; X RAY CRYSTAL STRUCTURES;

CRYSTAL ATOMIC STRUCTURE;

ALPHA N ACETYLGALACTOSAMINIDASE; CALCIUM ION; CARBON; GLYCOSIDASE; PROTON; SULFUR; BACTERIAL PROTEIN; COENZYME; ENZYME INHIBITOR; HYBRID PROTEIN; LIGAND; METAL; N ACETYLGALACTOSAMINE; PROBIOTIC AGENT;

ARTICLE; BIFIDOBACTERIUM BIFIDUM; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; HYDROGEN BOND; LIGAND BINDING; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOPHILICITY; OXYGEN AFFINITY; PRIORITY JOURNAL; PROTEIN DOMAIN; STRUCTURE ANALYSIS; X RAY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTAGONISTS AND INHIBITORS; CHEMISTRY; COENZYME; CONSERVED SEQUENCE; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL; MUTATION; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STRUCTURAL HOMOLOGY; X RAY CRYSTALLOGRAPHY;

ACETYLGALACTOSAMINE; ALPHA-N-ACETYLGALACTOSAMINIDASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIFIDOBACTERIUM BIFIDUM; BINDING SITES; CATALYTIC DOMAIN; COENZYMES; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME INHIBITORS; GLYCOSIDE HYDROLASES; LIGANDS; METALS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROBIOTICS; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 85025171014     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M117.777391     Document Type: Article

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