Molecular cloning and catalytic mechanism of a novel glycosphingolipid- degrading β-N-acetylgalactosaminidase from Paenibacillus sp. TS12

Journal of Biological Chemistry

Volumn 286, Issue 16, 2011, Pages 14065-14072

  Sumida, Tomomi ^a   Fujimoto, Ken ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AMINO ACID RESIDUES; CATALYTIC MECHANISMS; GLYCOSPHINGOLIPIDS; MOLECULAR CLONING; OXAZOLINIUM; POINT MUTATIONS; POTENT INHIBITOR; PROTON DONORS; SECONDARY STRUCTURES; SEQUENCE SIMILARITY; SUBSTRATE-ASSISTED CATALYSIS; THIAZOLINE;

AMINO ACIDS; CATALYSIS; CATALYSTS; CLONING; ENZYMES; ESCHERICHIA COLI; SUBSTRATES;

QUANTUM CHEMISTRY;

4 NITROPHENYL BETA N ACETYLGALACTOSAMINIDE; 4 NITROPHENYL BETA N ACETYLGLUCOSAMINIDE; ACETAMIDE; ASPARTIC ACID; BETA N ACETYLGALACTOSAMINIDASE; ENZYME INHIBITOR; GALACTOSAMINE; GANGLIOTRIOSYLCERAMIDE; GLOBOTETRAOSYLCERAMIDE; GLUCOSAMINE DERIVATIVE; GLUTAMIC ACID; N ACETYLGALACTOSAMINE THIAZOLINE; N ACETYLGLUCOSAMINE THIAZOLINE; OXAZOLINIUM; RECOMBINANT BETA N ACETYLGALACTOSAMINIDASE; STABILIZING AGENT; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BETA-N-ACETYL-GALACTOSAMINIDASE; BINDING, COMPETITIVE; CATALYSIS; CATALYTIC DOMAIN; CLONING, MOLECULAR; GLYCOSPHINGOLIPIDS; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; PAENIBACILLUS; POINT MUTATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; PAENIBACILLUS SP.;

EID: 79954614002     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.182592     Document Type: Article

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