Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha

Journal of Bioscience and Bioengineering

Volumn 124, Issue 5, 2017, Pages 487-492

  Numamoto, Minori ^a   Maekawa, Hiromi ^a   Kaneko, Yoshinobu ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

CRISPR Cas9; Genome editing; Methylotrophic yeast; Ogataea polymorpha; tRNA processing

Indexed keywords

EFFICIENCY; GENE EXPRESSION; POLYMERASE CHAIN REACTION;

CRISPR/CAS9; EDITING TOOLS; GENETIC MANIPULATIONS; HANSENULA POLYMORPHA; HOMOLOGOUS RECOMBINATION; METHYLOTROPHIC YEASTS; OGATAEA POLYMORPHA; TRANSFORMANTS;

YEAST;

CASPASE 9; CRISPR ASSOCIATED PROTEIN; RNA; SINGLE GUIDE RNA; TRANSFER RNA; UNCLASSIFIED DRUG; FUNGAL RNA;

ADE12 GENE; ARTICLE; CELL TRANSFORMATION; CONTROLLED STUDY; FUNGAL STRAIN; GENE; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; NONHUMAN; OGATAEA; OGATAEA POLYMORPHA; PHO1 GENE; PHO11 GENE; PHO84 GENE; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; TRANSCRIPTION REGULATION; BUDDING YEAST; CRISPR CAS SYSTEM; FUNGAL GENE; FUNGAL GENOME; GENE EDITING; GENETICS; PROCEDURES;

CRISPR-CAS SYSTEMS; GENE EDITING; GENES, FUNGAL; GENOME, FUNGAL; PLASMIDS; PROMOTER REGIONS, GENETIC; RNA, FUNGAL; RNA, TRANSFER; SACCHAROMYCETALES;

EID: 85021728831     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2017.06.001     Document Type: Article

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