Controlling microbial PHB synthesis via CRISPRi

Applied Microbiology and Biotechnology

Volumn 101, Issue 14, 2017, Pages 5861-5867

  Li, Dan ^a   Lv, Li ^a   Chen, Jin Chun ^a   Chen, Guo Qiang ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Cas9; CRISPRi; Molecular weight; PHA synthase; PhaC; PHB; Polyhydroxyalkanoates; Synthetic biology

Indexed keywords

BACTERIA; BINS; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; GENES; POLYPROPYLENES; TRANSCRIPTION; VOLCANIC ROCKS;

CAS9; CRISPRI; PHA SYNTHASES; PHAC; POLYHYDROXYALKANOATES; SYNTHETIC BIOLOGY;

MOLECULAR WEIGHT;

BACTERIAL ENZYME; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYBUTYRATE; POLYMER; UNCLASSIFIED DRUG; ACYLTRANSFERASE; BACTERIAL PROTEIN; GUIDE RNA; HYDROXYBUTYRIC ACID; POLY-BETA-HYDROXYBUTYRATE POLYMERASE; POLYHYDROXYALKANOIC ACID;

BACTERIUM; BIOTECHNOLOGY; ETHYLENE; EUKARYOTE; GENE; GENE EXPRESSION; GENOME; HYDROCARBON; HYDROXYL RADICAL; MICROBIAL ACTIVITY; MOLECULAR ANALYSIS; POLYMER; PROKARYOTE; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; MOLECULAR WEIGHT; NONHUMAN; PHAC GENE; CHEMISTRY; CRISPR CAS SYSTEM; GENETICS; METABOLISM; MOLECULAR CLONING; OPERON; PROCEDURES; SYNTHETIC BIOLOGY;

CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

ACYLTRANSFERASES; BACTERIAL PROTEINS; CLONING, MOLECULAR; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GENE EXPRESSION; HYDROXYBUTYRATES; MOLECULAR WEIGHT; OPERON; POLYHYDROXYALKANOATES; RNA, GUIDE; SYNTHETIC BIOLOGY;

EID: 85020514019     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-017-8374-6     Document Type: Article

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