Efficient molecular weight control of bacterially synthesized polyesters by alcohol supplementation

Journal of Chemical Technology and Biotechnology

Volumn 89, Issue 7, 2014, Pages 1110-1114

  Thomson, Nicholas M ^a   Hiroe, Ayaka ^b   Tsuge, Takeharu ^b   Summers, David K ^c   Sivaniah, Easan ^a,d  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

Chain transfer; E. coli; Ethanol; Methanol; Molecular weight; Polyhydroxyalkanoate; Polyhydroxybutyrate

Indexed keywords

ESCHERICHIA COLI; ETHANOL; METHANOL; POLYETHYLENE GLYCOLS; WEIGHT CONTROL;

CHAIN TRANSFER; CHAIN TRANSFER AGENTS; COMMERCIAL POTENTIAL; E. COLI; MECHANICAL PERFORMANCE; POLY(3-HYDROXYBUTYRATE); POLY-HYDROXYALKANOATE; POLYHYDROXYBUTYRATE;

MOLECULAR WEIGHT;

2 PROPANOL; ACETIC ACID; ALCOHOL; GLYCEROL; MACROGOL 200; METHANOL; POLY(3 HYDROXYBUTYRIC ACID);

ARTICLE; BACTERIAL GROWTH; CARBON SOURCE; CELL GROWTH; CULTURE MEDIUM; DENSITY; ESCHERICHIA COLI; MOLECULAR WEIGHT; NONHUMAN; SUPPLEMENTATION; SYNTHESIS; WEIGHT CONTROL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84901690766     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.4198     Document Type: Article

References (20)

1. Agus J, Kahar P, Abe H, Doi Y and Tsuge T, Altered expression of polyhydroxyalkanoate synthase gene and its effect on poly[(R)-3-hydroxybutyrate] synthesis in recombinant Escherichia coli. Polym Degrad Stab 91:1645-1650 (2006).
2. Kahar P, Agus J, Kikkawa Y, Taguchi K, Doi Y and Tsuge T, Effective production and kinetic characterization of ultra-high-molecular-weight poly [(R)-3-hydroxybutyrate] in recombinant Escherichia coli. Poly Degrad Stab 87:161-169 (2005).
3. Agus J, Kahar P, Abe H, Doi Y and Tsuge T, Molecular weight characterization of poly[(R)-3-hydroxybutyrate] synthesized by genetically engineered strains of Escherichia coli. Polym Degrad Stab 91:1138-1146 (2006).
4. Kusaka S, Abe H, Lee SY and Doi Y, Molecular mass of poly[(R)-3-hydroxybutyric acid] produced in a recombinant Escherichia coli. Appl Microbiol Biotechnol 47:140-143 (1997).
5. Sim SJ, Snell KD, Hogan SA, Stubbe J, Rha C and Sinskey AJ, PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat Biotechnol 15:63-67 (1997).
6. Hiroe A, Tsuge K, Nomura CT, Itaya M and Tsuge T, Rearrangement of gene order in the phaCAB operon leads to effective production of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] in genetically engineered Escherichia coli}. Appl Environ Microbiol 78:3177-3184 (2012).
7. Thomson NM, Saika A, Ushimaru K, Sangiambut S, Tsuge T, Summers DK and Sivaniah E, Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones. Appl Environ Microbiol 79:1948-1955 (2013).
8. Ha CS and Cho WJ, Miscibility, properties, and biodegradability of microbial polyester containing blends. Prog Polym Sci 27:759-809 (2002).
9. Grassie N, Murray EJ and Holmes PA. The thermal degradation of poly(-(D)-β-hydroxybutyric acid): Part 2 - changes in molecular weight. Polym Degrad Stab 6:95-103 (1984).
10. Scandola M, Mazzocchetti L, Focarete ML, Kawalec M, Kurcok P, Adamus G and Kowalczuk M, Production of low molecular weight polyhydroxyalkanoate oligomer and/or polymer used as building blocks for synthesis and modification of biodegradable polymer, involves reacting polyhydroxyalkanoate and carbonate salt. Patent No. 2010044112 (2010).
11. Gao X, Chen J, Wu Q and Chen G, Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels. Curr Opin Biotechnol 22:768-774 (2011).
12. Madden LA, Anderson AJ, Shah DT and Asrar J, Chain termination in polyhydroxyalkanoate synthesis: involvement of exogenous hydroxy-compounds as chain transfer agents. Int J Biol Macromol 25:43-53 (1999).
13. Ushimaru K, Sangiambut S, Thomson NM, Sivaniah E and Tsuge T, New insights into activation and substrate recognition of polyhydroxyalkanoate synthase from Ralstonia eutropha. Appl Microbiol Biotechnol 97:1175-1182 (2013).
14. Sangiambut S, Channon K, Thomson NM, Sato S, Tsuge T, Doi Y and Sivaniah E, A robust route to enzymatically functional, hierarchically self-assembled peptide frameworks. Adv Mater 25:2661-2665 (2013).
15. Shi F, Ashby RD and Gross RA, Use of poly(ethylene glycol)s to regulate poly(3-hydroxybutyrate) molecular weight during Alcaligenes eutrophus cultivations. Macromol 29:7753-7758 (1996).
16. Tomizawa S, Saito Y, Hyakutake M, Nakamura Y, Abe H and Tsuge T, Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent. Appl Microbiol Biotechnol 87:1427-1435 (2010).
17. Taidi B, Anderson AJ, Dawes EA and Byrom D, Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus. Appl Microbiol Biotechnol 40:786-790 (1994).
18. Suzuki T, Deguchi H, Yamane T, Shimizu S and Gekko K, Control of molecular weight of poly-β-hydroxybutyric acid produced in fed-batch culture of Protomonas extorquens. Appl Microbiol Biotechnol 27:487-491 (1988).
19. Mothes G, Schnorpfeil C and Ackermann J-U, Production of PHB from crude glycerol. Eng Life Sci 7:475-479 (2007).
20. Ashby RD, Shi F and Gross RA, A tunable switch to regulate the synthesis of low and high molecular weight microbial polyesters. Biotechnol Bioeng 62:106-113 (1999).