Protein digestomic analysis reveals the bioactivity of deer antler velvet in simulated gastrointestinal digestion

Food Research International

Volumn 96, Issue , 2017, Pages 182-190

  Yu, Yang ^a,b   Jin, Yan ^a   Wang, Fangjun ^a   Yan, Jiaze ^a   Qi, Yanxia ^b   Ye, Mingliang ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b DALIAN OCEAN UNIVERSITY   (China)

Author keywords

Antler velvet; Bioactivity; In vitro digestion; LC MS MS; Peptides; Protein digestomics

Indexed keywords

BIOACTIVITY; PEPTIDES; SILK;

ANTLER VELVET; BIOACTIVE COMPONENTS; DIPEPTIDYL PEPTIDASE IV; GASTROINTESTINAL DIGESTION; IN-VITRO BIOACTIVITY; IN-VITRO DIGESTIONS; LC-MS/MS; PROLYL ENDOPEPTIDASE;

PROTEINS;

DIPEPTIDYL PEPTIDASE IV INHIBITOR; PEPTIDE FRAGMENT; PROLYL ENDOPEPTIDASE; PROTEIN; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR;

ANIMAL; ANTLER; CHEMISTRY; DEER; DIGESTION; DRUG EFFECT; HYDROLYSIS; INTESTINAL SECRETIONS; LIQUID CHROMATOGRAPHY; MALE; METABOLISM; PROCEDURES; PROTEIN DEGRADATION; PROTEOMICS; STOMACH JUICE; TANDEM MASS SPECTROMETRY;

ANIMALS; ANTLERS; CHROMATOGRAPHY, LIQUID; DEER; DIGESTION; DIPEPTIDYL-PEPTIDASE IV INHIBITORS; GASTRIC JUICE; HYDROLYSIS; INTESTINAL SECRETIONS; MALE; PEPTIDE FRAGMENTS; PROTEINS; PROTEOLYSIS; PROTEOMICS; SERINE ENDOPEPTIDASES; SERINE PROTEINASE INHIBITORS; TANDEM MASS SPECTROMETRY;

EID: 85017154513     PISSN: 09639969     EISSN: 18737145     Source Type: Journal    
DOI: 10.1016/j.foodres.2017.04.002     Document Type: Article

References (36)

1. Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:1–2 (1976), 248–254.
2. Cahlikova, L., Hulova, L., Hrabinova, M., Chlebek, J., Hostalkova, A., Adamcova, M. Macakova, K., Isoquinoline alkaloids as prolyl oligopeptidase inhibitors. Fitoterapia 103 (2015), 192–196.
3. Cox, H.D., Eichner, D., Detection of human insulin-like growth factor-1 in deer antler velvet supplements. Rapid Communications in Mass Spectrometry 27:19 (2013), 2170–2178.
4. Drucker, D.J., Nauck, M.A., The incretin system: Glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors in type 2 diabetes. Lancet 368:9548 (2006), 1696–1705.
5. Du, C.N., Min, A.Y., Kim, H.J., Shin, S.K., Yu, H.N., Sohn, E.J. Kim, M.R., Deer bone extract prevents against scopolamine-induced memory impairment in mice. Journal of Medicinal Food 18:2 (2015), 157–165.
6. Ebner, J., Arslan, A.A., Fedorova, M., Hoffmann, R., Kucukcetin, A., Pischetsrieder, M., Peptide profiling of bovine kefir reveals 236 unique peptides released from caseins during its production by starter culture or kefir grains. Journal of Proteomics 117 (2015), 41–57.
7. Fulop, V., Bocskei, Z., Polgar, L., Prolyl oligopeptidase: An unusual beta-propeller domain regulates proteolysis. Cell 94:94 (1998), 161–170.
8. Gao, L.A., Tao, D.Y., Shan, Y.C., Liang, Z., Zhang, L.H., Huo, Y.S., Zhang, Y.K., HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 878:32 (2010), 3370–3374.
9. Garcia-Horsman, J.A., Mannisto, P.T., Venalainen, J.I., On the role of prolyl oligopeptidase in health and disease. Neuropeptides 41:1 (2007), 1–24.
10. Gass, J., Khosla, C., Prolyl endopeptidases. Cellular and Molecular Life Sciences 64:3 (2007), 345–355.
11. Jeon, B., Kim, S., Lee, S., Park, P., Sung, S., Kim, J., Moon, S., Effect of antler growth period on the chemical composition of velvet antler in sika deer (Cervus nippon). Mammalian Biology - Zeitschrift für Säugetierkunde 74:5 (2009), 374–380.
12. Jin, Y., Yan, J.Z., Yu, Y., Qi, Y.X., Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC–MS/MS and in silico analysis. Journal of Functional Foods 18 (2015), 344–357.
13. Jin, Y., Yu, Y., Qi, Y., Wang, F., Yan, J., Zou, H., Peptide profiling and the bioactivity character of yogurt in the simulated gastrointestinal digestion. Journal of Proteomics 141 (2016), 24–46.
14. Kierdorf, U., Li, C.Y., Price, J.S., Improbable appendages: Deer antler renewal as a unique case of mammalian regeneration. Seminars in Cell & Developmental Biology 20:5 (2009), 535–542.
15. Kim, K.H., Kim, K.S., Choi, B.J., Chung, K.H., Chang, Y.C., Lee, S.D. Kim, C.H., Anti-bone resorption activity of deer antler aqua- acupunture, the pilose antler of Cervus korean Temminck var. mantchuricus Swinhoe (Nokyong) in adjuvant-induced arthritic rats. Journal of Ethnopharmacology 96:3 (2005), 497–506.
16. Lafarga, T., O'Connor, P., Hayes, M., In silico methods to identify meat-derived prolyl endopeptidase inhibitors. Food Chemistry 175:15 (2015), 337–343.
17. Lee, S.H., Yang, H.W., Ding, Y., Wang, Y., Jeon, Y.J., Moon, S.H. Sung, S.H., Anti-inflammatory effects of enzymatic hydrolysates of velvet antler in raw 264.7 cells in vitro and zebrafish model. EXCLI Journal 14 (2015), 1122–1132.
18. Lee, M.R., Yun, B.S., Zhang, D.L., Liu, L., Wang, Z., Wang, C.L. Sung, C., Effect of aqueous antler extract on scopolamine-induced memory impairment in mice and antioxidant activities. Food Science and Biotechnology 19:3 (2010), 655–661.
19. Li, C., Harper, A., Puddick, J., Wang, W., McMahon, C., Proteomes and signalling pathways of antler stem cells. PloS One 7:1 (2012), 1–11.
20. Mika, N., Zorn, H., Ruhl, M., Prolyl-specific peptidases for applications in food protein hydrolysis. Applied Microbiology and Biotechnology 99:19 (2015), 7837–7846.
21. Park, H.J., Lee, D.H., Park, S.G., Lee, S.C., Cho, S., Kim, H.K. Park, B.C., Proteome analysis of red deer antlers. Proteomics 4:4 (2004), 3642–3653.
22. Picariello, G., Mamone, G., Nitride, C., Addeo, F., Ferranti, P., Protein digestomics: Integrated platforms to study food-protein digestion and derived functional and active peptides. TrAC Trends in Analytical Chemistry 52:12 (2013), 120–134.
23. Rosenblum, J.S., Kozarich, J.W., Proyl peptidases: A serine protease subfamily with high potential for drug discovery. Current Opinion in Chemical Biology 7:4 (2003), 496–504.
24. Saavedra, L., Hebert, E.M., Minahk, C., Ferranti, P., An overview of “omic” analytical methods applied in bioactive peptide studies. Food Research International 54:1 (2013), 925–934.
25. Sánchez-Rivera, L., Martínez-Maqueda, D., Cruz-Huerta, E., Miralles, B., Recio, I., Peptidomics for discovery, bioavailability and monitoring of dairy bioactive peptides. Food Research International 63 (2014), 170–181.
26. Sayd, T., Chambon, C., Santé-Lhoutellier, V., Quantification of peptides released during in vitro digestion of cooked meat. Food Chemistry 197:Part B (2016), 1311–1323.
27. Sila, A., Martinez-Alvarez, O., Haddar, A., Carmen Gomez-Guillen, M., Nasri, M., Pilar Montero, M., Bougatef, A., Recovery, viscoelastic and functional properties of Barbel skin gelatine: Investigation of anti-DPP-IV and anti-prolyl endopeptidase activities of generated gelatine polypeptides. Food Chemistry 168 (2015), 478–486.
28. Sui, Z., Yuan, H., Liang, Z., Zhao, Q., Wu, Q., Xia, S. Zhang, Y., An activity-maintaining sequential protein extraction method for bioactive assay and proteome analysis of velvet antlers. Talanta 107:2 (2013), 189–194.
29. Wu, F., Li, H., Jin, L., Li, X., Ma, Y., You, J. Xu, Y., Deer antler base as a traditional Chinese medicine: A review of its traditional uses, chemistry and pharmacology. Journal of Ethnopharmacology 145:2 (2013), 403–415.
30. Xin, J.L., Zhang, Y., Zhang, L.Z., Lin, Y., Zhou, Q.L., Comparison of structure and biological activity of natural polypeptide from velvet antlers of Cervus elaphus with those of synthesized polypeptide. Chemical Research in Chinese Universities 29:5 (2013), 924–928.
31. Zha, E., Li, X., Li, D., Guo, X., Gao, S., Yue, X., Immunomodulatory effects of a 3.2kDa polypeptide from velvet antler of Cervus nippon Temminck. International Immunopharmacology 16:2 (2013), 210–213.
32. Zhang, Z., Liu, X., Duan, L., Li, X., Zhang, Y., Zhou, Q., The effects of velvet antler polypeptides on the phenotype and related biological indicators of osteoarthritic rabbit chondrocytes. Acta Biochimica Polonica 58:3 (2011), 297–302.
33. Zhao, L., Luo, Y.C., Wang, C.T., Ji, B.P., Antioxidant activity of protein hydrolysates from aqueous extract of velvet antler (Cervus elaphus) as influenced by molecular weight and enzymes. Natural Product Communications 6:11 (2011), 1683–1688.
34. Zhao, L., Pei, R.S., Ji, B.P., Luo, Y.C., Zhang, D., Xu, Z.Y., Jia, X.N., Antioxidant activity of aqueous extract fractions of velvet antler (Cervus elaphus Linnaeus). Journal of Food and Drug Analysis 18:5 (2010), 319–327.
35. Zhou, R., Li, S., In vitro antioxidant analysis and characterisation of antler velvet extract. Food Chemistry 114:4 (2009), 1321–1327.
36. Zhu, J., Sun, Z., Cheng, K., Chen, R., Ye, M., Xu, B. Zou, H., Comprehensive mapping of protein N-glycosylation in human liver by combining hydrophilic interaction chromatography and hydrazide chemistry. Journal of Proteome Research 13:3 (2014), 1713–1721.