-
1
-
-
0034213573
-
Do more complex organisms have a greater proportion of membrane proteins in their genomes?
-
Stevens, T. J. & Arkin, I. T. Do more complex organisms have a greater proportion of membrane proteins in their genomes? Proteins 39, 417–420 (2000).
-
(2000)
Proteins
, vol.39
, pp. 417-420
-
-
Stevens, T.J.1
Arkin, I.T.2
-
2
-
-
34247893770
-
Structural genomics and drug discovery
-
Lundstrom, K. Structural genomics and drug discovery. J. Cell. Mol. Med. 11, 224–238 (2007).
-
(2007)
J. Cell. Mol. Med.
, vol.11
, pp. 224-238
-
-
Lundstrom, K.1
-
3
-
-
0031740601
-
Hydrophobic interactions of peptides with membrane interfaces
-
White, S. H. & Wimley, W. C. Hydrophobic interactions of peptides with membrane interfaces. Biochim. Biophys. Acta. 1376, 339–352 (1998).
-
(1998)
Biochim. Biophys. Acta.
, vol.1376
, pp. 339-352
-
-
White, S.H.1
Wimley, W.C.2
-
4
-
-
58249084813
-
Genetic analysis of G protein-coupled receptor expression in Escherichia coli: Inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor
-
Skretas, G. & Georgiou, G. Genetic analysis of G protein-coupled receptor expression in Escherichia coli: inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor. Biotechnol. Bioeng. 102, 357–367 (2009).
-
(2009)
Biotechnol. Bioeng.
, vol.102
, pp. 357-367
-
-
Skretas, G.1
Georgiou, G.2
-
5
-
-
59949092470
-
Genetic selection system for improving recombinant membrane protein expression in E. coli
-
Massey-Gendel, E. et al. Genetic selection system for improving recombinant membrane protein expression in E. coli. Protein Sci. 18, 372–383 (2009).
-
(2009)
Protein Sci
, vol.18
, pp. 372-383
-
-
Massey-Gendel, E.1
-
6
-
-
33646568438
-
Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): Unique resources for biological research
-
Kitagawa, M. et al. Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12, 291–299 (2005).
-
(2005)
DNA Res
, vol.12
, pp. 291-299
-
-
Kitagawa, M.1
-
7
-
-
77956606290
-
Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli
-
Skretas, G. & Georgiou, G. Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli. Appl. Environ. Microbiol. 76, 5852–5859 (2010).
-
(2010)
Appl. Environ. Microbiol.
, vol.76
, pp. 5852-5859
-
-
Skretas, G.1
Georgiou, G.2
-
8
-
-
79960678169
-
Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli
-
Nannenga, B. L. & Baneyx, F. Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli. Protein Sci. 20, 1411–1420 (2011).
-
(2011)
Protein Sci
, vol.20
, pp. 1411-1420
-
-
Nannenga, B.L.1
Baneyx, F.2
-
9
-
-
79955953366
-
Suppressing glucose uptake and acetic acid production increases membrane protein overexpression in Escherichia coli
-
Backlund, E., Ignatushchenko, M. & Larsson, G. Suppressing glucose uptake and acetic acid production increases membrane protein overexpression in Escherichia coli. Microb. Cell Fact. 10, 35 (2011).
-
(2011)
Microb. Cell Fact.
, vol.10
-
-
Backlund, E.1
Ignatushchenko, M.2
Larsson, G.3
-
10
-
-
84925172140
-
De-convoluting the genetic adaptations of E. coli C41(DE3) in real time reveals how alleviating protein production stress improves yields
-
Schlegel, S., Genevaux, P. & de Gier, J. W. De-convoluting the genetic adaptations of E. coli C41(DE3) in real time reveals how alleviating protein production stress improves yields. Cell Rep. 10, 1758–1766 (2015).
-
(2015)
Cell Rep
, vol.10
, pp. 1758-1766
-
-
Schlegel, S.1
Genevaux, P.2
de Gier, J.W.3
-
11
-
-
55749088241
-
Tuning Escherichia coli for membrane protein overexpression
-
Wagner, S. et al. Tuning Escherichia coli for membrane protein overexpression. Proc. Natl. Acad. Sci. USA 105, 14371–14376 (2008).
-
(2008)
Proc. Natl. Acad. Sci. USA
, vol.105
, pp. 14371-14376
-
-
Wagner, S.1
-
12
-
-
0030593468
-
Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels
-
Miroux, B. & Walker, J. E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260, 289–298 (1996).
-
(1996)
J. Mol. Biol.
, vol.260
, pp. 289-298
-
-
Miroux, B.1
Walker, J.E.2
-
13
-
-
1542779645
-
A general approach for heterologous membrane protein expression in Escherichia coli: The uncoupling protein, UCP1, as an example
-
Shaw, A. Z. & Miroux, B. A general approach for heterologous membrane protein expression in Escherichia coli: the uncoupling protein, UCP1, as an example. Methods Mol. Biol. 228, 23–35 (2003).
-
(2003)
Methods Mol. Biol.
, vol.228
, pp. 23-35
-
-
Shaw, A.Z.1
Miroux, B.2
-
14
-
-
70350641005
-
Genome evolution and adaptation in a long-term experiment with Escherichia coli
-
Barrick, J. E. et al. Genome evolution and adaptation in a long-term experiment with Escherichia coli. Nature 461, 1243–1247 (2009).
-
(2009)
Nature
, vol.461
, pp. 1243-1247
-
-
Barrick, J.E.1
-
15
-
-
0023042283
-
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
-
Studier, F. W. & Moffatt, B. A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113–130 (1986).
-
(1986)
J. Mol. Biol.
, vol.189
, pp. 113-130
-
-
Studier, F.W.1
Moffatt, B.A.2
-
16
-
-
0025283867
-
W. Use of T7 RNA polymerase to direct expression of cloned genes
-
Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60–89 (1990).
-
(1990)
Methods Enzymol
, vol.185
, pp. 60-89
-
-
Studier, F.W.1
Rosenberg, A.H.2
Dunn, J.J.3
Dubendorff, J.4
-
17
-
-
70350686881
-
Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)
-
Jeong, H. et al. Genome sequences of Escherichia coli B strains REL606 and BL21(DE3). J. Mol. Biol. 394, 644–652 (2009).
-
(2009)
J. Mol. Biol.
, vol.394
, pp. 644-652
-
-
Jeong, H.1
-
18
-
-
3543149006
-
The toxicity of recombinant proteins in Escherichia coli: A comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3)
-
Dumon-Seignovert, L., Cariot, G. & Vuillard, L. The toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3). Protein Expr. Purif. 37, 203–206 (2004).
-
(2004)
Protein Expr. Purif.
, vol.37
, pp. 203-206
-
-
Dumon-Seignovert, L.1
Cariot, G.2
Vuillard, L.3
-
19
-
-
34848823739
-
Consequences of membrane protein overexpression in Escherichia coli
-
Wagner, S. et al. Consequences of membrane protein overexpression in Escherichia coli. Mol. Cell Proteomics 6, 1527–1550 (2007).
-
(2007)
Mol. Cell Proteomics
, vol.6
, pp. 1527-1550
-
-
Wagner, S.1
-
20
-
-
84867268590
-
Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3)
-
Schlegel, S. et al. Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3). J. Mol. Biol. 423, 648–659 (2012).
-
(2012)
J. Mol. Biol.
, vol.423
, pp. 648-659
-
-
Schlegel, S.1
-
21
-
-
80052887804
-
Use of GFP fusions for the isolation of Escherichia coli strains for improved production of different target recombinant proteins
-
Alfasi, S. et al. Use of GFP fusions for the isolation of Escherichia coli strains for improved production of different target recombinant proteins. J. Biotechnol. 156, 11–21 (2011).
-
(2011)
J. Biotechnol.
, vol.156
, pp. 11-21
-
-
Alfasi, S.1
-
22
-
-
84866342658
-
First insights into the unexplored two-component system YehU/YehT in Escherichia coli
-
Kraxenberger, T., Fried, L., Behr, S. & Jung, K. First insights into the unexplored two-component system YehU/YehT in Escherichia coli. J. Bacteriol. 194, 4272–4284 (2012).
-
(2012)
J. Bacteriol.
, vol.194
, pp. 4272-4284
-
-
Kraxenberger, T.1
Fried, L.2
Behr, S.3
Jung, K.4
-
23
-
-
0021364453
-
D-ribose metabolism in Escherichia coli K-12: Genetics, regulation, and transport
-
Lopilato, J. E., Garwin, J. L., Emr, S. D., Silhavy, T. J. & Beckwith, J. R. D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. J. Bacteriol. 158, 665–673 (1984).
-
(1984)
J. Bacteriol.
, vol.158
, pp. 665-673
-
-
Lopilato, J.E.1
Garwin, J.L.2
Emr, S.D.3
Silhavy, T.J.4
Beckwith, J.R.5
-
24
-
-
0035046481
-
Mechanisms causing rapid and parallel losses of ribose catabolism in evolving populations of Escherichia coli B
-
Cooper, V. S., Schneider, D., Blot, M. & Lenski, R. E. Mechanisms causing rapid and parallel losses of ribose catabolism in evolving populations of Escherichia coli B. J. Bacteriol. 183, 2834–2841 (2001).
-
(2001)
J. Bacteriol.
, vol.183
, pp. 2834-2841
-
-
Cooper, V.S.1
Schneider, D.2
Blot, M.3
Lenski, R.E.4
-
25
-
-
0035161027
-
IS186 insertion at a hot spot in the lon promoter as a basis for lon protease deficiency of Escherichia coli B: Identification of a consensus target sequence for IS186 transposition
-
saiSree, L., Reddy, M. & Gowrishankar, J. IS186 insertion at a hot spot in the lon promoter as a basis for lon protease deficiency of Escherichia coli B: identification of a consensus target sequence for IS186 transposition. J. Bacteriol. 183, 6943–6946 (2001).
-
(2001)
J. Bacteriol.
, vol.183
, pp. 6943-6946
-
-
Saisree, L.1
Reddy, M.2
Gowrishankar, J.3
-
26
-
-
0021266784
-
lon gene product of Escherichia coli is a heat-shock protein
-
Phillips, T. A., VanBogelen, R. A. & Neidhardt, F. C. lon gene product of Escherichia coli is a heat-shock protein. J Bacteriol 159, 283–287 (1984).
-
(1984)
J Bacteriol
, vol.159
, pp. 283-287
-
-
Phillips, T.A.1
Vanbogelen, R.A.2
Neidhardt, F.C.3
-
30
-
-
0035078316
-
Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
-
Bernstein, H. D. & Hyndman, J. B. Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli. J. Bacteriol. 183, 2187–2197 (2001).
-
(2001)
J. Bacteriol.
, vol.183
, pp. 2187-2197
-
-
Bernstein, H.D.1
Hyndman, J.B.2
-
31
-
-
33645454462
-
Optimization of membrane protein overexpression and purification using GFP fusions
-
Drew, D., Lerch, M., Kunji, E., Slotboom, D. J. & de Gier, J. W. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 3, 303–313 (2006).
-
(2006)
Nat. Methods
, vol.3
, pp. 303-313
-
-
Drew, D.1
Lerch, M.2
Kunji, E.3
Slotboom, D.J.4
de Gier, J.W.5
-
32
-
-
13644267280
-
Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase
-
Vethanayagam, J. G. & Flower, A. M. Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase. Microb. Cell Fact. 4, 3 (2005).s
-
(2005)
Microb. Cell Fact.
, vol.4
, pp. 3
-
-
Vethanayagam, J.G.1
Flower, A.M.2
-
33
-
-
0023464708
-
Vectors for selective expression of cloned DNAs by T7 RNA polymerase
-
Rosenberg, A. H. et al. Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56, 125–135 (1987).
-
(1987)
Gene
, vol.56
, pp. 125-135
-
-
Rosenberg, A.H.1
-
34
-
-
0003845223
-
-
Schrödinger, Inc., Date of access: 20/04/2015
-
DeLano, W. L. The PyMOL Molecular Graphic System. Schrödinger, Inc. http://www.pymol.org/(2002) Date of access: 20/04/2015.
-
(2002)
The Pymol Molecular Graphic System
-
-
Delano, W.L.1
-
35
-
-
0029938053
-
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
-
Lewis, M. et al. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271, 1247–1254 (1996).
-
(1996)
Science
, vol.271
, pp. 1247-1254
-
-
Lewis, M.1
-
36
-
-
34249951731
-
Structural analysis of lac repressor bound to allosteric effectors
-
Daber, R., Stayrook, S., Rosenberg, A. & Lewis, M. Structural analysis of lac repressor bound to allosteric effectors. J. Mol. Biol. 370, 609–619 (2007).
-
(2007)
J. Mol. Biol.
, vol.370
, pp. 609-619
-
-
Daber, R.1
Stayrook, S.2
Rosenberg, A.3
Lewis, M.4
-
37
-
-
0026344978
-
Arginine 197 of lac repressor contributes significant energy to inducer binding. Confirmation of homology to periplasmic sugar binding proteins
-
Spotts, R. O., Chakerian, A. E. & Matthews, K. S. Arginine 197 of lac repressor contributes significant energy to inducer binding. Confirmation of homology to periplasmic sugar binding proteins. J. Biol. Chem. 266, 22998–23002 (1991).
-
(1991)
J. Biol. Chem.
, vol.266
, pp. 22998-23002
-
-
Spotts, R.O.1
Chakerian, A.E.2
Matthews, K.S.3
-
38
-
-
0028076771
-
Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as “spacers” which do not require a specific sequence
-
Markiewicz, P., Kleina, L. G., Cruz, C., Ehret, S. & Miller, J. H. Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as “spacers” which do not require a specific sequence. J. Mol. Biol. 240, 421–433 (1994).
-
(1994)
J. Mol. Biol.
, vol.240
, pp. 421-433
-
-
Markiewicz, P.1
Kleina, L.G.2
Cruz, C.3
Ehret, S.4
Miller, J.H.5
-
39
-
-
0141888960
-
Improving protein folding efficiency by directed evolution using the GFP folding reporter
-
Waldo, G. S. Improving protein folding efficiency by directed evolution using the GFP folding reporter. Methods Mol. Biol. 230, 343–359 (2003).
-
(2003)
Methods Mol. Biol.
, vol.230
, pp. 343-359
-
-
Waldo, G.S.1
-
40
-
-
85136082713
-
An optimized strategy for genome assembly of sanger/pyrosequencing hybrid data using available software
-
Jeong, H. & Kim, J. F. An optimized strategy for genome assembly of sanger/pyrosequencing hybrid data using available software. Genomics Inform. 6, 87–90 (2008).
-
(2008)
Genomics Inform
, vol.6
, pp. 87-90
-
-
Jeong, H.1
Kim, J.F.2
-
41
-
-
0031955116
-
Consed: A graphical tool for sequence finishing
-
Gordon, D., Abajian, C. & Green, P. Consed: a graphical tool for sequence finishing. Genome Res. 8, 195–202 (1998).
-
(1998)
Genome Res
, vol.8
, pp. 195-202
-
-
Gordon, D.1
Abajian, C.2
Green, P.3
-
42
-
-
0031978181
-
Base-calling of automated sequencer traces using phred. II. Error probabilities
-
Ewing, B. & Green, P. Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 8, 186–194 (1998).
-
(1998)
Genome Res
, vol.8
, pp. 186-194
-
-
Ewing, B.1
Green, P.2
-
43
-
-
79959438245
-
Systematic exploration of error sources in pyrosequencing flowgram data
-
Balzer, S., Malde, K. & Jonassen, I. Systematic exploration of error sources in pyrosequencing flowgram data. Bioinformatics 27, i304–309 (2011).
-
(2011)
Bioinformatics
, vol.27
, pp. i304-i309
-
-
Balzer, S.1
Malde, K.2
Jonassen, I.3
-
44
-
-
0034612342
-
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
-
Datsenko, K. A. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97, 6640–6645 (2000).
-
(2000)
Proc. Natl. Acad. Sci. USA
, vol.97
, pp. 6640-6645
-
-
Datsenko, K.A.1
Wanner, B.L.2
-
45
-
-
49249139249
-
Rapid and efficient construction of markerless deletions in the Escherichia coli genome
-
Yu, B. J. et al. Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Res. 36, e84 (2008).
-
(2008)
Nucleic Acids Res
, vol.36
, pp. e84
-
-
Yu, B.J.1
|