Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli

Applied and Environmental Microbiology

Volumn 76, Issue 17, 2010, Pages 5852-5859

  Skretas, Georgios ^a,b,c   Georgiou, George ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^c INSTITUTE OF BIOLOGY   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL MEMBRANE PROTEINS; CO-EXPRESSION; DNA-BINDING PROTEIN; E. COLI; EFFICIENT PRODUCTION; G PROTEIN COUPLED RECEPTORS; GENETIC APPROACH; GENETIC SELECTION; INTEGRAL MEMBRANE PROTEINS; LACTAMASES; MEMBRANE PROTEINS; OPEN READING FRAME; PRODUCTION STRAIN;

BACTERIOLOGY; BIOLOGICAL MEMBRANES; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING;

PROTEINS;

AMPICILLIN; ANTIINFECTIVE AGENT; BETA LACTAMASE; ESCHERICHIA COLI PROTEIN; G PROTEIN COUPLED RECEPTOR; HYBRID PROTEIN; MEMBRANE PROTEIN;

COLIFORM BACTERIUM; EXPERIMENTAL STUDY; GENE EXPRESSION; GENETIC ENGINEERING; MEMBRANE; PROTEIN;

ARTICLE; BIOTECHNOLOGY; DRUG ANTAGONISM; ESCHERICHIA COLI; GENETIC SELECTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; METHODOLOGY; MICROBIAL GENETICS;

AMPICILLIN; ANTI-BACTERIAL AGENTS; BETA-LACTAMASES; BIOTECHNOLOGY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETICS, MICROBIAL; HUMANS; MEMBRANE PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; SELECTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77956606290     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00963-10     Document Type: Article

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