Crystal structure of the lactose operon represser and its complexes with DNA and inducer

Science

Volumn 271, Issue 5253, 1996, Pages 1247-1254

  Lewis, Mitchell ^a   Chang, Geoffrey ^a   Horton, Nancy C ^b,d   Kercher, Michele A ^b   Pace, Helen C ^b   Schumacher, Maria A ^c   Brennan, Richard G ^c   Lu, Ponzy ^b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^d PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CYCLIC AMP BINDING PROTEIN;

ARTICLE; BACTERIAL GENETICS; CRYSTAL STRUCTURE; DNA SEQUENCE; ESCHERICHIA COLI; GENE CONTROL; LACTOSE OPERON; NONHUMAN; PRIORITY JOURNAL; REPRESSOR GENE;

ALLOSTERIC REGULATION; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYCLIC AMP RECEPTOR PROTEIN; DNA, BACTERIAL; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; ISOPROPYL THIOGALACTOSIDE; LAC OPERON; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OPERATOR REGIONS (GENETICS); POINT MUTATION; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; REPRESSOR PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029938053     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5253.1247     Document Type: Article

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80. We thank S. L Ginell, L J. Keefe, C. E. Schutt, R. Shigeta Jr., and A. Luptak for technical assistance and D. C Rees and M. J Bennett for discussions, and the Argonne National Laboratory Structural Biology Center and CHESS and MacCHESS for access to synchrotron x-ray facilities. Supported by National Institutes of Health (NIH) grant GM-44617 and an Army Research Office grant DAAL03-92-G-0713, NIH Cell and Molecular Biology training grants (N C H and M A.K ), and NIH Molecular Biophysics training grant 2-T32-GM082745 (G.C). The Argonne National Laboratory Structural Biology Center beamline, X-8-C of the National Synchrotron Light Source, is supported by the Department of Energy, Office of Health and Environmental Research, and by the NIH-NCRR grant P41-RR06017. The Cornell High Energy Synchrotron Source is supported by the National Science Foundation (DMR-9311772). Mac-CHESS is supported by a NIH grant RR-016416. Atomic coordinates have been deposited in the Protein Data Bank with accession numbers 1LBG (lac DNA), 1LBH (lac IPTG), and 1LBI (lac Apo).