Continuous genetic recording with self-targeting CRISPR-Cas in human cells

Science

Volumn 353, Issue 6304, 2016, Pages

  Perli, Samuel D ^a,b,c   Cui, Cheryl H ^a,b   Lu, Timothy K ^a,b,c  

^a USA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c Department of Electrical Engineering and Computer Science   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; DNA ENDONUCLEASE CAS9; DOUBLE STRANDED DNA; GUIDE RNA; RED FLUORESCENT PROTEIN; SELF TARGETING GUIDE RNA; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; DNA; ENDONUCLEASE;

CELLS AND CELL COMPONENTS; CHEMICAL CUE; ENZYME ACTIVITY; GENE; GENE EXPRESSION; GENETIC ANALYSIS; HOMINID; INSTRUMENTATION; MOLECULAR ANALYSIS;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA REPAIR; DOUBLE STRANDED DNA BREAK; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; HUMAN; HUMAN CELL; MOUSE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION; CELL TRACKING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; HEK293 CELL LINE; MEMORY; METABOLISM; NUCLEOTIDE SEQUENCE; PROCEDURES; SINGLE CELL ANALYSIS;

MAMMALIA; STREPTOCOCCUS PYOGENES;

BACTERIAL PROTEINS; BASE SEQUENCE; CELL TRACKING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA; ENDONUCLEASES; HEK293 CELLS; HUMANS; MEMORY; MUTAGENESIS; RNA, GUIDE; SINGLE-CELL ANALYSIS;

EID: 85015591616     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aag0511     Document Type: Article

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