Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

Nature Protocols

Volumn 11, Issue 9, 2016, Pages 1724-1743

  Broutier, Laura ^a   Andersson Rolf, Amanda ^b   Hindley, Christopher J ^a   Boj, Sylvia F ^c   Clevers, Hans ^d   Koo, Bon Kyoung ^b   Huch, Meritxell ^a,b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c Foundation Hubrecht Organoid Technology (HUB)   (Netherlands)

^d UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL EXPANSION; CELL SELF-RENEWAL; CONTROLLED STUDY; DNA MODIFICATION; ENDOCRINE CELL; EXTRACELLULAR MATRIX; FEMALE; GENETIC MANIPULATION; GENETIC STABILITY; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LIVER CELL; MALE; MOUSE; NONHUMAN; PANCREAS CELL; PRIORITY JOURNAL; ADULT STEM CELL; ANIMAL; CELL CULTURE TECHNIQUE; CYTOLOGY; GENETIC ENGINEERING; LIVER; METABOLISM; ORGANOID; PANCREAS; PROCEDURES;

ADULT STEM CELLS; ANIMALS; CELL CULTURE TECHNIQUES; CELL SELF RENEWAL; FEMALE; GENETIC ENGINEERING; HUMANS; LIVER; MALE; MICE; ORGANOIDS; PANCREAS;

EID: 85014751112     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.097     Document Type: Article

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