CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

BioTechniques

Volumn 58, Issue 4, 2015, Pages 161-170

  Wang, Jia Wang ^a   Wang, Amy ^a   Li, Kunyu ^a   Wang, Bangmei ^a   Jin, Shunqian ^b   Reiser, Michelle ^a   Lockey, Richard F ^a,c  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Cas9; CRISPR cleavage; Gibson cloning; Large vector; Single guide RNA

Indexed keywords

CRISPR ASSOCIATED ENZYME 9; CRISPR ASSOCIATED PROTEIN; UNCLASSIFIED DRUG; CASPASE 9; RESTRICTION ENDONUCLEASE;

ARTICLE; CELL TRANSFORMATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSCRIPTION; GIBSON CLONING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE METABOLISM; POLYMERASE CHAIN REACTION; PROTEIN CLEAVAGE; RNA CLEAVAGE; RNA SYNTHESIS; CHEMISTRY; CRISPR CAS SYSTEM; GENETICS; GENOME; PHYSIOLOGY; PLASMID; PROCEDURES;

ANIMALIA;

CASPASE 9; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; DNA RESTRICTION ENZYMES; GENOME; PLASMIDS;

EID: 84927511493     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114261     Document Type: Article

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