Enhancement of 8-methoxypsoralen topical delivery via nanosized niosomal vesicles: Formulation development, in vitro and in vivo evaluation of skin deposition

International Journal of Pharmaceutics

Volumn 517, Issue 1-2, 2017, Pages 256-268

  Kassem, Ahmed Alaa ^a   Abd El Alim, Sameh Hosam ^a   Asfour, Marwa Hasanein ^a  

^a NATIONAL RESEARCH CENTRE   (Egypt)

Author keywords

8 methoxypsoralen; Confocal laser scanning microscopy; Hydrogel; Niosomes; Skin permeation; Topical delivery

Indexed keywords

ALCOHOL; BUFFER; CHOLESTEROL; METHOXSALEN; METHYLCELLULOSE; NIOSOME; PHOSPHATE; RHODAMINE; SORBITAN PALMITATE; SORBITAN STEARATE; DRUG CARRIER; HYDROGEL; LIPOSOME;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; CONFOCAL LASER SCANNING MICROSCOPY; CONTROLLED STUDY; DISPERSION; DRUG EFFICACY; DRUG FORMULATION; DRUG SAFETY; DRUG STABILITY; DRUG STORAGE; HYDROGEL; IN VITRO STUDY; IN VIVO STUDY; MALE; NANOPHARMACEUTICS; NONHUMAN; PARTICLE SIZE; PH; PRIORITY JOURNAL; RAT; REFRIGERATION; SKIN PENETRATION; SKIN WATER LOSS; SUSTAINED DRUG RELEASE; TOPICAL TREATMENT; TRANSMISSION ELECTRON MICROSCOPY; ZETA POTENTIAL; ANIMAL; CHEMISTRY; DRUG DELIVERY SYSTEM; DRUG RELEASE; FLOW KINETICS; SKIN ABSORPTION; ULTRASTRUCTURE;

ANIMALS; DRUG CARRIERS; DRUG DELIVERY SYSTEMS; DRUG LIBERATION; DRUG STABILITY; HYDROGEL; HYDROGEN-ION CONCENTRATION; LIPOSOMES; MALE; METHOXSALEN; PARTICLE SIZE; RATS; RHEOLOGY; SKIN ABSORPTION;

EID: 85006868995     PISSN: 03785173     EISSN: 18733476     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2016.12.018     Document Type: Article

References (94)

1. Abd El-Alim, S.H., Kassem, A.A., Basha, M., Proniosomes as a novel drug carrier system for buccal delivery of benzocaine. J. Drug Deliv. Sci. Technol. 24 (2014), 452–458.
2. Abdelbary, A.A., AbouGhaly, M.H., Design and optimization of topical methotrexate loaded niosomes for enhanced management of psoriasis: application of box-behnken design, in-vitro evaluation and in-vivo skin deposition study. Int. J. Pharm. 485 (2015), 235–243.
3. Abdelbary, G., El-Gendy, N., Niosome-encapsulated gentamicin for ophthalmic controlled delivery. AAPS PharmSciTech 9 (2008), 740–747.
4. Abdelkader, H., Alani, A.W., Alany, R.G., Recent advances in non-ionic surfactant vesicles (niosomes): self-assembly, fabrication, characterization, drug delivery applications and limitations. Drug Deliv. 21 (2014), 87–100.
5. Abraham, W., Downing, D.T., Interaction between corneocytes and stratum corneum lipid liposomes in vitro. Biochim. Biophys. Acta (BBA) – Biomembr. 1021 (1990), 119–125.
6. Akhtar, N., Arkvanshi, S., Bhattacharya, S.S., Verma, A., Pathak, K., Preparation and evaluation of a buflomedil hydrochloride niosomal patch for transdermal delivery. J. Liposome Res. 25 (2015), 191–201.
7. Allam, A., Fetih, G., Sublingual fast dissolving niosomal films for enhanced bioavailability and prolonged effect of metoprolol tartrate. Drug Des. Dev. Ther. 10 (2016), 2421–2433.
8. Alsarra, I.A., Bosela, A.A., Ahmed, S.M., Mahrous, G.M., Proniosomes as a drug carrier for transdermal delivery of ketorolac. Eur. J. Pharm. Biopharm. 59 (2005), 485–490.
9. Alvi, I.A., Madan, J., Kaushik, D., Sardana, S., Pandey, R.S., Ali, A., Comparative study of transfersomes, liposomes, and niosomes for topical delivery of 5-fluorouracil to skin cancer cells: preparation, characterization, in-vitro release, and cytotoxicity analysis. Anticancer Drugs 22 (2011), 774–782.
10. Antunes, F.E., Gentile, L., Rossi, C.O., Tavano, L., Ranieri, G.A., Gels of Pluronic F127 and nonionic surfactants from rheological characterization to controlled drug permeation. Colloids Surf. B: Biointerfaces 87 (2011), 42–48.
11. Arzani, G., Haeri, A., Daeihamed, M., Bakhtiari-Kaboutaraki, H., Dadashzadeh, S., Niosomal carriers enhance oral bioavailability of carvedilol: effects of bile salt-enriched vesicles and carrier surface charge. Int. J. Nanomed. 10 (2015), 4797–4813.
12. Baillie, A.J., Florence, A.T., Hume, L.R., Muirhead, G.T., Rogerson, A., The preparation and properties of niosomes–non-ionic surfactant vesicles. J. Pharm. Pharmacol. 37 (1985), 863–868.
13. Bal, S.M., Ding, Z., van Riet, E., Jiskoot, W., Bouwstra, J.A., Advances in transcutaneous vaccine delivery: do all ways lead to Rome?. J. Controlled Release 148 (2010), 266–282.
14. Balakrishnan, P., Shanmugam, S., Lee, W.S., Lee, W.M., Kim, J.O., Oh, D.H., Kim, D.D., Kim, J.S., Yoo, B.K., Choi, H.G., Woo, J.S., Yong, C.S., Formulation and in vitro assessment of minoxidil niosomes for enhanced skin delivery. Int. J. Pharm. 377 (2009), 1–8.
15. Baroli, B., Lopez-Quintela, M.A., Delgado-Charro, M.B., Fadda, A.M., Blanco-Mendez, J., Microemulsions for topical delivery of 8-methoxsalen. J. Controlled Release 69 (2000), 209–218.
16. Basha, M., Abd El-Alim, S.H., Kassem, A.A., El Awdan, S., Awad, G., Benzocaine loaded solid lipid nanoparticles: formulation design, in vitro and in vivo evaluation of local anesthetic effect. Curr. Drug Deliv. 12 (2015), 680–692.
17. Borowska, K., Wolowiec, S., Glowniak, K., Sieniawska, E., Radej, S., Transdermal delivery of 8-methoxypsoralene mediated by polyamidoamine dendrimer G2. 5 and G3. 5–in vitro and in vivo study. Int. J. Pharm. 436 (2012), 764–770.
18. Borowska, K., Wolowiec, S., Rubaj, A., Glowniak, K., Sieniawska, E., Radej, S., Effect of polyamidoamine dendrimer G3 and G4 on skin permeation of 8-methoxypsoralene–in vivo study. Int. J. Pharm. 426 (2012), 280–283.
19. Coviello, T., Trotta, A.M., Marianecci, C., Carafa, M., Di Marzio, L., Rinaldi, F., Di Meo, C., Alhaique, F., Matricardi, P., Gel-embedded niosomes: preparation, characterization and release studies of a new system for topical drug delivery. Colloids Surf. B: Biointerfaces 125 (2015), 291–299.
20. Dufes, C., Gaillard, F., Uchegbu, I.F., Schatzlein, A.G., Olivier, J.C., Muller, J.M., Glucose-targeted niosomes deliver vasoactive intestinal peptide (VIP) to the brain. Int. J. Pharm. 285 (2004), 77–85.
21. du Plessis, J., Ramachandran, C., Weiner, N., Muller, D.G., The influence of lipid composition and lamellarity of liposomes on the physical stability of liposomes upon storage. Int. J. Pharm. 127 (1996), 273–278.
22. El-Badry, M., Fetih, G., Fathalla, D., Shakeel, F., Transdermal delivery of meloxicam using niosomal hydrogels: in vitro and pharmacodynamic evaluation. Pharm. Dev. Technol. 20 (2015), 820–826.
23. El-Laithy, H.M., Shoukry, O., Mahran, L.G., Novel sugar esters proniosomes for transdermal delivery of vinpocetine: preclinical and clinical studies. Eur. J. Pharm. Biopharm. 77 (2011), 43–55.
24. El-Menshawe, S.F., Hussein, A.K., Formulation and evaluation of meloxicam niosomes as vesicular carriers for enhanced skin delivery. Pharm. Dev. Technol. 18 (2013), 779–786.
25. El-Ridy, M.S., Abdelbary, A., Essam, T., El-Salam, R.M., Kassem, A.A., Niosomes as a potential drug delivery system for increasing the efficacy and safety of nystatin. Drug Dev. Ind. Pharm. 37 (2011), 1491–1508.
26. El-Ridy, M.S., Abdelbary, A., Nasr, E.A., Khalil, R.M., Mostafa, D.M., El-Batal, A.I., Abd El-Alim, S.H., Niosomal encapsulation of the antitubercular drug, pyrazinamide. Drug Dev. Ind. Pharm. 37 (2011), 1110–1118.
27. El-Ridy, M.S., Yehia, S.A., Kassem, M.A., Mostafa, D.M., Nasr, E.A., Asfour, M.H., Niosomal encapsulation of ethambutol hydrochloride for increasing its efficacy and safety. Drug Deliv. 22 (2015), 21–36.
28. El-Say, K.M., Abd-Allah, F.I., Lila, A.E., Hassan, A.S., Kassem, A.E., Diacerein niosomal gel for topical delivery: development, in vitro and in vivo assessment. J. Liposome Res. 26 (2016), 57–68.
29. ElMeshad, A.N., Tadros, M.I., Transdermal delivery of an anti-cancer drug via w/o emulsions based on alkyl polyglycosides and lecithin: design, characterization, and in vivo evaluation of the possible irritation potential in rats. AAPS PharmSciTech 12 (2011), 1–9.
30. Fang, J.Y., Fang, C.L., Liu, C.H., Su, Y.H., Lipid nanoparticles as vehicles for topical psoralen delivery: solid lipid nanoparticles (SLN) versus nanostructured lipid carriers (NLC). Eur. J. Pharm. Biopharm. 70 (2008), 633–640.
31. Garg, B.J., Garg, N.K., Beg, S., Singh, B., Katare, O.P., Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: formulation optimization, in vitro evaluation and preclinical assessment. J. Drug Target. 24 (2016), 233–246.
32. Goyal, G., Garg, T., Malik, B., Chauhan, G., Rath, G., Goyal, A.K., Development and characterization of niosomal gel for topical delivery of benzoyl peroxide. Drug Deliv. 22 (2015), 1027–1042.
33. Grundmann-Kollmann, M., Tegeder, I., Ochsendorf, F.R., Zollner, T.M., Ludwig, R., Kaufmann, R., Podda, M., Kinetics and dose-response of photosensitivity in cream psoralen plus ultraviolet A photochemotherapy: comparative in vivo studies after topical application of three standard preparations. Br. J. Dermatol. 144 (2001), 991–995.
34. Guinedi, A.S., Mortada, N.D., Mansour, S., Hathout, R.M., Preparation and evaluation of reverse-phase evaporation and multilamellar niosomes as ophthalmic carriers of acetazolamide. Int. J. Pharm. 306 (2005), 71–82.
35. Hofland, H.E., van der Geest, R., Bodde, H.E., Junginger, H.E., Bouwstra, J.A., Estradiol permeation from nonionic surfactant vesicles through human stratum corneum in vitro. Pharm. Res. 11 (1994), 659–664.
36. Israelachvili, J.N., Intermolecular and Surface Forces: With Applications to Colloidal and Biological Systems. 1985, Academic Press London, Orlando.
37. Japanese Pharmacopoeia, Methoxsalen, Japanese Pharmacopoeia Official Monographs Part I. 14 ed., 2001, 611.
38. Junyaprasert, V.B., Teeranachaideekul, V., Supaperm, T., Effect of charged and non-ionic membrane additives on physicochemical properties and stability of niosomes. AAPS PharmSciTech 9 (2008), 851–859.
39. Junyaprasert, V.B., Singhsa, P., Suksiriworapong, J., Chantasart, D., Physicochemical properties and skin permeation of span 60/Tween 60 niosomes of ellagic acid. Int. J. Pharm. 423 (2012), 303–311.
40. Kakkar, S., Kaur, I.P., A novel nanovesicular carrier system to deliver drug topically. Pharm. Dev. Technol. 18 (2013), 673–685.
41. Kassem, M.A., El-Sawy, H.S., Abd-Allah, F.I., Abdelghany, T.M., El-Say, K.M., Maximizing the therapeutic efficacy of imatinib mesylate-loaded niosomes on human colon adenocarcinoma using box-behnken design. J. Pharm. Sci. 106 (2017), 111–122.
42. Khalil, R.M., Abdelbary, G.A., Basha, M., Awad, G.E., El-Hashemy, H.A., Enhancement of lomefloxacin Hcl ocular efficacy via niosomal encapsulation: in vitro characterization and in vivo evaluation. J. Liposome Res., 2016, 1–12.
43. Khan, M.I., Madni, A., Ahmad, S., Mahmood, M.A., Rehman, M., Ashfaq, M., Formulation design and characterization of a non-ionic surfactant based vesicular system for the sustained delivery of a new chondroprotective agent. Braz. J. Pharm. Sci. 51 (2015), 607–615.
44. Komatsu, H., Higaki, K., Okamoto, H., Miyagawa, K., Hashida, M., Sezaki, H., Preservative activity and in vivo percutaneous penetration of butylparaben entrapped in liposomes. Chem. Pharm. Bull. 34 (1986), 3415–3422.
45. Kumari, S., Pathak, K., Cavamax W7 composite psoralen ethosomal gel versus cavamax W7 psoralen solid complex gel for topical delivery: a comparative evaluation. Int. J. Pharm. Investig. 3 (2013), 171–182.
46. Lapolla, W., Yentzer, B.A., Bagel, J., Halvorson, C.R., Feldman, S.R., A review of phototherapy protocols for psoriasis treatment. J. Am. Acad. Dermatol. 64 (2011), 936–949.
47. Lee, C.H., Moturi, V., Lee, Y., Thixotropic property in pharmaceutical formulations. J. Controlled Release 136 (2009), 88–98.
48. Loutfy, M.A., Hassan, M.A., Methoxsalen. Anal. Profiles Drug Subs. 9 (1981), 427–454.
49. Mali, N., Darandale, S., Vavia, P., Niosomes as a vesicular carrier for topical administration of minoxidil: formulation and in vitro assessment. Drug Delivery Transl. Res. 3 (2013), 587–592.
50. Manca, M.L., Manconi, M., Nacher, A., Carbone, C., Valenti, D., Maccioni, A.M., Sinico, C., Fadda, A.M., Development of novel diolein-niosomes for cutaneous delivery of tretinoin: influence of formulation and in vitro assessment. Int. J. Pharm. 477 (2014), 176–186.
51. Manconi, M., Sinico, C., Valenti, D., Lai, F., Fadda, A.M., Niosomes as carriers for tretinoin. III. A study into the in vitro cutaneous delivery of vesicle-incorporated tretinoin. Int. J. Pharm. 311 (2006), 11–19.
52. Manosroi, A., Wongtrakul, P., Manosroi, H., Sakai, J., Sugawara, F., Yuasa, M., Abe, M., Characterization of vesicles prepared with various non-ionic surfactants mixed with cholesterol. Colloids Surf. B: Biointerfaces 30 (2003), 129–138.
53. Manosroi, A., Khanrin, P., Lohcharoenkal, W., Werner, R.G., Gِtz, F., Manosroi, W., Manosroi, J., Transdermal absorption enhancement through rat skin of gallidermin loaded in niosomes. Int. J. Pharm. 392 (2010), 304–310.
54. Manosroi, A., Khanrin, P., Werner, R.G., Gotz, F., Manosroi, W., Manosroi, J., Entrapment enhancement of peptide drugs in niosomes. J. Microencapsulation 27 (2010), 272–280.
55. Manosroi, A., Chankhampan, C., Manosroi, W., Manosroi, J., Transdermal absorption enhancement of papain loaded in elastic niosomes incorporated in gel for scar treatment. Eur. J. Pharm. Sci. 48 (2013), 474–483.
56. Marianecci, C., Paolino, D., Celia, C., Fresta, M., Carafa, M., Alhaique, F., Non-ionic surfactant vesicles in pulmonary glucocorticoid delivery: characterization and interaction with human lung fibroblasts. J. Controlled Release 147 (2010), 127–135.
57. Marianecci, C., Di Marzio, L., Rinaldi, F., Celia, C., Paolino, D., Alhaique, F., Esposito, S., Carafa, M., Niosomes from 80s to present: the state of the art. Adv. Colloid Interface Sci. 205 (2014), 187–206.
58. Martin-Villena, M.J., Fernandez-Campos, F., Calpena-Campmany, A.C., Bozal-de Febrer, N., Ruiz-Martinez, M.A., Clares-Naveros, B., Novel microparticulate systems for the vaginal delivery of nystatin: development and characterization. Carbohydr. Polym. 94 (2013), 1–11.
59. Martindale, Methoxsalen. Sweetman, S.C., (eds.) Martindale: The Complete Drug Reference, 37 ed., 2011, Martindale, London, 1746–1749.
60. Moghassemi, S., Parnian, E., Hakamivala, A., Darzianiazizi, M., Vardanjani, M.M., Kashanian, S., Larijani, B., Omidfar, K., Uptake and transport of insulin across intestinal membrane model using trimethyl chitosan coated insulin niosomes. Mater. Sci. Eng. 46 (2015), 333–340.
61. Muzzalupo, R., Tavano, L., Cassano, R., Trombino, S., Ferrarelli, T., Picci, N., A new approach for the evaluation of niosomes as effective transdermal drug delivery systems. Eur. J. Pharm. Biopharm. 79 (2011), 28–35.
62. Nasseri, B., Effect of cholesterol and temperature on the elastic properties of niosomal membranes. Int. J. Pharm. 300 (2005), 95–101.
63. Ning, M., Guo, Y., Pan, H., Chen, X., Gu, Z., Preparation, in vitro and in vivo evaluation of liposomal/niosomal gel delivery systems for clotrimazole. Drug Dev. Ind. Pharm. 31 (2005), 375–383.
64. Ozkan, I., Kose, O., Ozmen, I., Arca, E., Efficacy and safety of non-laser, targeted UVB phototherapy alone and in combination with psoralen gel or calcipotriol ointment in the treatment of localized, chronic, plaque-type psoriasis. Int. J. Dermatol. 51 (2012), 609–613.
65. Patel, N.A., Patel, N.J., Patel, R.P., Comparative development and evaluation of topical gel and cream formulations of psoralen. Drug Discov. Ther. 3 (2009), 234–242.
66. Paulsson, M., Edsman, K., Controlled drug release from gels using surfactant aggregates. II. vesicles formed from mixtures of amphiphilic drugs and oppositely charged surfactants. Pharm. Res. 18 (2001), 1586–1592.
67. Peppas, N.A., Sahlin, J.J., A simple equation for the description of solute release. III. Coupling of diffusion and relaxation. Int. J. Pharm. 57 (1989), 169–172.
68. Rentel, C.O., Bouwstra, J.A., Naisbett, B., Junginger, H.E., Niosomes as a novel peroral vaccine delivery system. Int. J. Pharm. 186 (1999), 161–167.
69. Sapam, R., Agrawal, S., Dhali, T.K., Systemic PUVA vs. narrowband UVB in the treatment of vitiligo: a randomized controlled study. Int. J. Dermatol. 51 (2012), 1107–1115.
70. Schmid, J., Prox, A., Zipp, H., Koss, F.W., The use of stable isotopes to prove the saturable first-pass effect of methoxsalen. Biol. Mass Spectrom. 7 (1980), 560–564.
71. Shahiwala, A., Misra, A., Studies in topical application of niosomally entrapped Nimesulide. J. Pharm. Pharm. Sci. 5 (2002), 220–225.
72. Shaker, D.S., Shaker, M.A., Hanafy, M.S., Cellular uptake, cytotoxicity and in-vivo evaluation of tamoxifen citrate loaded niosomes. Int. J. Pharm. 493 (2015), 285–294.
73. Singla, S., Harikumar, S., Aggarwal, G., Proniosomes for effective topical delivery of clotrimazole: development, characterization, and performance evaluation. Asian J. Pharm. Sci. 7 (2012), 259–270.
74. Sinico, C., Valenti, D., Manconi, M., Lai, F., Fadda, A.M., Cutaneous delivery of 8-methoxypsoralen from liposomal and niosomal carriers. J. Drug Deliv. Sci. Technol. 16 (2006), 115–120.
75. Stern, R.S., Laird, N., The carcinogenic risk of treatments for severe psoriasis: photochemotherapy follow-up study. Cancer 73 (1994), 2759–2764.
76. Stern, R.S., Vakeva, L.H., Noncutaneous malignant tumors in the PUVA follow-up study: 1975–1996. J. Invest. Dermatol. 108 (1997), 897–900.
77. Uchegbu, I.F., Duncan, R., Niosomes containing N-(2-hydroxypropyl)methacrylamide copolymer-doxorubicin (PK1): effect of method of preparation and choice of surfactant on niosome characteristics and a preliminary study of body distribution. Int. J. Pharm. 155 (1997), 7–17.
78. Uchegbu, I.F., Vyas, S.P., Non-ionic surfactant based vesicles (niosomes) in drug delivery. Int. J. Pharm. 172 (1998), 33–70.
79. Ullah, S., Shah, M.R., Shoaib, M., Imran, M., Elhissi, A.M.A., Ahmad, F., Ali, I., Shah, S.W.A., Development of a biocompatible creatinine-based niosomal delivery system for enhanced oral bioavailability of clarithromycin. Drug Deliv., 2016, 1–12.
80. Verma, P., Pathak, K., Therapeutic and cosmeceutical potential of ethosomes: an overview. J. Adv. Pharm. Technol. Res. 1 (2010), 274–282.
81. Verma, P., Pathak, K., Nanosized ethanolic vesicles loaded with econazole nitrate for the treatment of deep fungal infections through topical gel formulation. Nanomed.: Nanotechnol. Biol. Med. 8 (2012), 489–496.
82. Verma, D.D., Verma, S., Blume, G., Fahr, A., Particle size of liposomes influences dermal delivery of substances into skin. Int. J. Pharm. 258 (2003), 141–151.
83. Volodkin, D., Mohwald, H., Voegel, J.C., Ball, V., Coating of negatively charged liposomes by polylysine: drug release study. J. Controlled Release 117 (2007), 111–120.
84. Vora, B., Khopade, A.J., Jain, N.K., Proniosome based transdermal delivery of levonorgestrel for effective contraception. J. Controlied Release 54 (1998), 149–165.
85. Wagh, V.D., Deshmukh, O.J., Itraconazole niosomes drug delivery system and its antimycotic activity against candida albicans. ISRN Pharm., 2012, 2012, 653465.
86. Wagner, M.E., Spoth, K.A., Kourkoutis, L.F., Rizvi, S.S., Stability of niosomes with encapsulated vitamin D3 and ferrous sulfate generated using a novel supercritical carbon dioxide method. J. Liposome Res. 26 (2016), 261–268.
87. Yoshioka, T., Sternberg, B., Florence, A.T., Preparation and properties of vesicles (niosomes) of sorbitan monoesters (Span 20, 40, 60 and 80) and a sorbitan triester (Span 85). Int. J. Pharm. 105 (1994), 1–6.
88. Zhang, H., Zhang, J., Streisand, J.B., Oral mucosal drug delivery: clinical pharmacokinetics and therapeutic applications. Clin. Pharmacokinet. 41 (2002), 661–680.
89. Zhang, Z., Tsai, P.C., Ramezanli, T., Michniak-Kohn, B.B., Polymeric nanoparticles-based topical delivery systems for the treatment of dermatological diseases. Wiley interdisciplinary reviews. Nanomed. Nanobiotechnol. 5 (2013), 205–218.
90. Zhang, Y.T., Shen, L.N., Wu, Z.H., Zhao, J.H., Feng, N.P., Comparison of ethosomes and liposomes for skin delivery of psoralen for psoriasis therapy. Int. J. Pharm. 471 (2014), 449–452.
91. Zhang, Y.T., Shen, L.N., Wu, Z.H., Zhao, J.H., Feng, N.P., Evaluation of skin viability effect on ethosome and liposome-mediated psoralen delivery via cell uptake. J. Pharm. Sci. 103 (2014), 3120–3126.
92. Zhang, Y.T., Shen, L.N., Zhao, J.H., Feng, N.P., Evaluation of psoralen ethosomes for topical delivery in rats by using in vivo microdialysis. Int. J. Nanomed. 9 (2014), 669–678.
93. Zhang, Y., Zhang, K., Wu, Z., Guo, T., Ye, B., Lu, M., Zhao, J., Zhu, C., Feng, N., Evaluation of transdermal salidroside delivery using niosomes via in vitro cellular uptake. Int. J. Pharm. 478 (2015), 138–146.
94. Zidan, A.S., Habib, M.J., Maximized mucoadhesion and skin permeation of anti-AIDS-loaded niosomal gels. J. Pharm. Sci. 103 (2014), 952–964.