Effect of Linker Length and Flexibility on the Clostridium thermocellum Esterase Displayed on Bacillus subtilis Spores

Applied Biochemistry and Biotechnology

Volumn 182, Issue 1, 2017, Pages 168-180

  Chen, Huayou ^a,b   Wu, Bangguo ^a   Zhang, Tianxi ^a   Jia, Jinru ^a   Lu, Jian ^a   Chen, Zhi ^a   Ni, Zhong ^a   Tan, Tianwei ^c  

^a JIANGSU UNIVERSITY   (China)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

Author keywords

Bacillus subtilis; Displayed; Esterase; Flexibility; Linker; Spore

Indexed keywords

BACTERIOLOGY; DIMETHYL SULFOXIDE; ENZYME ACTIVITY; ENZYMES; ESTERS; ORGANIC SOLVENTS;

BACILLUS SUBTILIS; DISPLAYED; ESTERASE; FLEXIBILITY; LINKER; SPORE;

PROTEINS;

DIMETHYL SULFOXIDE; DNA POLYMERASE; ESTERASE; HYBRID PROTEIN; METHANOL; ORGANIC SOLVENT; RECOMBINANT ENZYME; RESTRICTION ENDONUCLEASE; BACTERIAL PROTEIN;

AMINO ACID COMPOSITION; ARTICLE; BACILLUS SUBTILIS; BIOLOGICAL ACTIVITY; CLOSTRIDIUM THERMOCELLUM; ENZYME ACTIVITY; NONHUMAN; PH; PLASMID; PROTEIN DOMAIN; PROTEIN FUNCTION; SURFACE PROPERTY; TEMPERATURE; THERMOSTABILITY; BACTERIAL SPORE; CHEMISTRY; ENZYME ASSAY; ENZYME STABILITY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN ENGINEERING; PROTEIN MOTIF; STRUCTURE ACTIVITY RELATION;

AMINO ACID MOTIFS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CLONING, MOLECULAR; CLOSTRIDIUM THERMOCELLUM; DIMETHYL SULFOXIDE; ENZYME ASSAYS; ENZYME STABILITY; ESTERASES; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; METHANOL; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SPORES, BACTERIAL; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE;

EID: 85001578768     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-016-2318-y     Document Type: Article

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