SynLinker: An integrated system for designing linkers and synthetic fusion proteins

Bioinformatics

Volumn 31, Issue 22, 2015, Pages 3700-3702

  Liu, Chengcheng ^a   Chin, Ju Xin ^a   Lee, Dong Yup ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN;

AMINO ACID SEQUENCE; COMPUTER INTERFACE; COMPUTER PROGRAM; HUMAN; PROTEIN DATABASE; SYNTHESIS;

AMINO ACID SEQUENCE; DATABASES, PROTEIN; HUMANS; RECOMBINANT FUSION PROTEINS; SOFTWARE; USER-COMPUTER INTERFACE;

EID: 84947787900     PISSN: 13674803     EISSN: 14602059     Source Type: Journal    
DOI: 10.1093/bioinformatics/btv447     Document Type: Article

References (12)

1. Amet,N. et al. (2009) Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm. Res., 26, 523-528.
2. Bai,Y. et al. (2005) Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc. Natl. Acad. Sci. USA, 102, 7292-7296.
3. Chen,X. et al. (2012) Fusion protein linkers: property, design and functionality. Adv. Drug Deliv. Rev., 65, 1357-1369.
4. Cheng,T.M. et al. (2008) Structural assembly of two-domain proteins by rigid-body docking. BMC Bioinformatics, 9, 441.
5. Crasto,C.J. and Feng,J.A. (2000) LINKER: a program to generate linker sequences for fusion proteins. Protein Eng., 13, 309-312.
6. de Bold,M.K. et al. (2012) Characterization of a long-acting recombinant human serum albumin-atrial natriuretic factor (ANF) expressed in Pichia pastoris. Regul. Pept., 175, 7-10.
7. Feldman,H.J. and Hogue,C.W. (2000) A fast method to sample real protein conformational space. Proteins, 39, 112-131.
8. George,R.A. and Heringa,J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Eng., 15, 871-879.
9. Xue,F. et al. (2004) LINKER: a web server to generate peptide sequences with extended conformation. Nucleic Acids Res., 32, W562-W565.
10. Yu,K. et al. (2015) Synthetic fusion protein design and applications. Biotechnol. Adv., 33, 155-164.
11. Zhang,J. et al. (2009) Design and optimization of a linker for fusion protein construction. Prog. Nat. Sci., 19, 1197-1200.
12. Zhao,H.L. et al. (2008) Increasing the homogeneity, stability and activity of human serum albumin and interferon-alpha2b fusion protein by linker engineering. Protein Exp. Purif., 61, 73-77.