Insertion of the designed helical linker led to increased expression of Tf-based fusion proteins

Pharmaceutical Research

Volumn 26, Issue 3, 2009, Pages 523-528

  Amet, N ^a   Lee, H F ^a   Shen, W C ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Domain switch; Fusion protein; G CSF; Helical linker; HGH; Increased expression; Transferrin

Indexed keywords

ANTIBODY; GRANULOCYTE COLONY STIMULATING FACTOR; GRANULOCYTE COLONY STIMULATING FACTOR ANTIBODY; GROWTH HORMONE ANTIBODY; HUMAN GROWTH HORMONE; HYBRID PROTEIN; OLIGONUCLEOTIDE; RECOMBINANT HUMAN GROWTH HORMONE LEUCYLGLUTAMYLALANYL TETRAKIS(GLUTAMYLALANYLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMYLALANYL TETRAKIS(GLUTAMYLALANYLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMIC ACID TRANSFERRIN FUSION PROTEIN; RECOMBINANT HUMAN GROWTH HORMONE TRANSFERRIN FUSION PROTEIN; RECOMBINANT TRANSFERRIN GRANULOCYTE COLONY STIMULATING FACTOR FUSION PROTEIN; RECOMBINANT TRANSFERRIN LEUCYLGLUTAMYLALANYL TETRAKIS(GLUTAMYLALANYLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMYLALANYL(GLUTAMYLALAN YLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMIC ACID GRANULOCYTE COLONY STIMULATING FACTOR RECOMBINANT FUSION PROTEIN; TRANSFERRIN; TRANSFERRIN ANTIBODY; TRANSFERRIN LEUCYLGLUTAMYLALANYL TETRAKIS(GLUTAMYLALANYLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMYLALANYL TETRAKIS(GLUTAMYLALANYLALANYLALANYLLYSYL)ALANYLLEUCYLGLUTAMIC ACID HUMAN GROWTH HORMONE FUSION PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CARBOXY TERMINAL SEQUENCE; CELL PROLIFERATION; CELL STRAIN HEK293; COMPUTER PROGRAM; CONTROLLED STUDY; DRUG DESIGN; DRUG EFFECT; DRUG STRUCTURE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; RAT; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE, TUMOR; CELL PROLIFERATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; HUMAN GROWTH HORMONE; HUMANS; PEPTIDES; PROTEIN ENGINEERING; RATS; RECOMBINANT FUSION PROTEINS; TRANSFERRIN;

EID: 60049093597     PISSN: 07248741     EISSN: 1573904X     Source Type: Journal    
DOI: 10.1007/s11095-008-9767-0     Document Type: Article

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