Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 44, 2016, Pages E6749-E6756

  Stranges, P Benjamin ^a   Palla, Mirkó ^a,b   Kalachikov, Sergey ^c   Nivala, Jeff ^a   Dorwart, Michael ^d   Trans, Andrew ^d   Kumar, Shiv ^c   Porel, Mintu ^c   Chien, Minchen ^c   Tao, Chuanjuan ^c   Morozova, Irina ^c   Li, Zengmin ^c   Shi, Shundi ^c   Aberra, Aman ^e   Arnold, Cleoma ^d   Yang, Alexander ^d   Aguirre, Anne ^d   Harada, Eric T ^d   Korenblum, Daniel ^d   Pollard, James ^d   Bhat, Ashwini ^d   Gremyachinskiy, Dmitriy ^d   Bibillo, Arek ^d   Chen, Roger ^d   Davis, Randy ^d   Russo, James J ^c   Fuller, Carl W ^c,d   Roever, Stefan ^d   Ju, Jingyue ^c,f   Church, George M ^a,b,d   more..

^a HARVARD MEDICAL SCHOOL   (United States)

^b HARVARD UNIVERSITY   (United States)

^c Department of Earth and Environmental Sciences   (United States)

^d Genia Technologies   (United States)

^e ARIZONA STATE UNIVERSITY   (United States)

^f Columbia University College ^*  (United States)

Author keywords

Integrated electrode array; Nanopore sequencing; Polymer tagged nucleotides; Protein design; Single molecule detection

Indexed keywords

ALPHA HEMOLYSIN; CYSTEINE CONJUGATE; DIVALENT CATION; DNA POLYMERASE; MANGANESE; NUCLEOTIDE; POLYHISTIDINE TAG; POLYMER; PORIN; DNA DIRECTED DNA POLYMERASE;

CARBOXY TERMINAL SEQUENCE; COMPLEMENTARY METAL OXIDE SEMICONDUCTOR; CONFERENCE PAPER; DNA BASE COMPOSITION; DNA HAIRPIN; DNA SEQUENCE; DNA SYNTHESIS; ELECTRODE; LIPID BILAYER; MICHAELIS CONSTANT; NANOPORE; NANOPORE BASED SEQUENCING BY SYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SEMICONDUCTOR; SEQUENCE ALIGNMENT; STOICHIOMETRY; CHEMISTRY; DEVICES; DNA REPLICATION; EQUIPMENT DESIGN; HIGH THROUGHPUT SEQUENCING; METABOLISM; MOLECULAR MODEL; PROCEDURES;

DNA REPLICATION; DNA-DIRECTED DNA POLYMERASE; ELECTRODES; EQUIPMENT DESIGN; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MODELS, MOLECULAR; NANOPORES; NUCLEOTIDES; POLYMERS; PORINS;

EID: 84994376712     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1608271113     Document Type: Article

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