Unfoldase-mediated protein translocation through an α-hemolysin nanopore

Nature Biotechnology

Volumn 31, Issue 3, 2013, Pages 247-250

  Nivala, Jeff ^a   Marks, Douglas B ^a   Akeson, Mark ^a  

^a University of California Santa Cruz   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCING DEVICES; DNA TRANSLOCATION; MOLECULAR MOTORS; PROTEIN SEQUENCE ANALYSIS; PROTEIN TRANSLOCATION; SEQUENCE-DEPENDENT; SINGLE-MOLECULE; TECHNICAL REQUIREMENT;

BLOOD; DNA SEQUENCES; NANOPORES;

PROTEINS;

ALPHA HEMOLYSIN; ENDOPEPTIDASE CLPX;

AMINO ACID SEQUENCE; ARTICLE; HUMAN; ION CURRENT; NANOPORE; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN TRANSPORT; PROTEIN UNFOLDING;

ADENOSINE TRIPHOSPHATASES; BIOTECHNOLOGY; ENDOPEPTIDASE CLP; ESCHERICHIA COLI PROTEINS; HEMOLYSIN PROTEINS; LIPID BILAYERS; MODELS, MOLECULAR; MOLECULAR CHAPERONES; NANOPORES; PROTEIN TRANSPORT; PROTEIN UNFOLDING;

EID: 84875153533     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.2503     Document Type: Article

References (20)

1. Church, G.M., Deamer, D.W., Branton, D., Baldarelli, R. & Kasianowicz, J. Characterization of individual polymer molecules based on monomer-interface inter-action. US patent 5,795,782 (1998).
2. Cherf, G.M. et al. Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision. Nat. Biotechnol. 30, 344-348 (2012).
3. Manrao, E.A. et al. Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase. Nat. Biotechnol. 30, 349-353 (2012).
4. Mohammad, M.M., Prakash, S., Matouschek, A. & Movileanu, L. Controlling a single protein in a nanopore through electrostatic traps. J. Am. Chem. Soc. 130, 4081-4088 (2008).
5. Talaga, D.S. & Li, J. Single-molecule protein unfolding in solid state nanopores. J. Am. Chem. Soc. 131, 9287-9297 (2009).
6. Merstorf, C. et al. Wild type, mutant protein unfolding and phase transition detected by single-nanopore recording. ACS Chem. Biol. 7, 652-658 (2012).
7. Baker, T.A. & Sauer, R.T. ClpXP, an ATP-powered unfolding and protein-degradation machine. Biochim. Biophys. Acta 1823, 15-28 (2012).
8. Aubin-Tam, M.E. et al. Single-molecule protein unfolding and translocation by an ATP-fueled proteolytic machine. Cell 145, 257-267 (2011).
9. Maillard, R.A. et al. ClpX(P) generates mechanical force to unfold and translocate its protein substrates. Cell 145, 459-469 (2011).
10. Simon, S.M. & Blobel, G. A protein-conducting channel in the endoplasmic reticulum. Cell 65, 371-380 (1991).
11. Johnson, E.S., Schweinhorst, I., Dohmen, R.J. & Blobel, G. The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer. EMBO J. 16, 5509-5519 (1997).
12. Sheng, W. & Liao, X. Solution structure of a yeast ubiquitin-like protein Smt3: the role of structurally less defined sequences in protein-protein recognitions. Protein Sci. 11, 1482-1491 (2002).
13. Gottesman, S., Roche, E., Zhou, Y. & Sauer, R.T. The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. Genes Dev. 12, 1338-1347 (1998).
14. Kim, Y. et al. Dynamics of substrate denaturation and translocation by the ClpXP degradation machine. Mol. Cell 5, 639-648 (2000).
15. Christensen, C. et al. Effect of charge, topology and orientation of the electric field on the interaction of peptides with the α-hemolysin pore. J. Pept. Sci. 17, 726-734 (2011).
16. Movileanu, L. Interrogating single proteins through nanopores: challenges and opportunities. Trends Biotechnol. 27, 333-341 (2009).
17. Oukhaled, G. et al. Unfolding of proteins and long transient conformations detected by single nanopore recording. Phys. Rev. Lett. 98, 158101 (2007).
18. Olasagasti, F. et al. Replication of individual DNA molecules under electronic control using a protein nanopore. Nat. Nanotechnol. 5, 798-806 (2010).
19. Martin, A., Baker, T.A. & Sauer, R.T. Rebuilt AAA+ motors reveal operating principles for ATP-fuelled machines. Nature 437, 1115-1120 (2005).
20. Kenniston, J.A., Baker, T.A., Fernandez, J.M. & Sauer, R.T. Linkage between ATP consumption and mechanical unfolding during the protein processing reactions of an AAA+ degradation machine. Cell 114, 511-520 (2003).