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Mapping posttranscriptional regulation of the human glycome uncovers microRNA defining the glycocode
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Maps the lectin binding profile of the NCI-60 cell lines. Identifies microRNA as a key regulator of cellular glycosylation.
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4•• Agrawal, P., Kurcon, T., Pilobello, K.T., Rakus, J.F., Koppolu, S., Liu, Z., Batista, B.S., Eng, W.S., Hsu, K.L., Liang, Y., Mahal, L.K., Mapping posttranscriptional regulation of the human glycome uncovers microRNA defining the glycocode. Proc Natl Acad Sci U S A 111 (2014), 4338–4343 Maps the lectin binding profile of the NCI-60 cell lines. Identifies microRNA as a key regulator of cellular glycosylation.
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84878644998
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Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology
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Analyzed the spread of O-glycosylation sites using 12 different human cell types that lack the ability to extend O-GalNAc type carbohydrates (SimpleCells). The study identifies 3000 O-glycosites on 600 glycoproteins.
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5•• Steentoft, C., Vakhrushev, S.Y., Joshi, H.J., Kong, Y., Vester-Christensen, M.B., Schjoldager, K.T., Lavrsen, K., Dabelsteen, S., Pedersen, N.B., Marcos-Silva, L., Gupta, R., et al. Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology. EMBO J 32 (2013), 1478–1488 Analyzed the spread of O-glycosylation sites using 12 different human cell types that lack the ability to extend O-GalNAc type carbohydrates (SimpleCells). The study identifies 3000 O-glycosites on 600 glycoproteins.
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Steentoft, C.1
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Precision mapping of an in vivo n-glycoproteome reveals rigid topological and sequence constraints
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Describes the use of ‘filter aided sample preparation’ to map 6367 N-glycosylation sites on 2352 proteins in mouse tissue and blood plasma. Besides the consensus N-X-S/T (X≠P) sequon for N-glycosylation, the study also shows rare N-X-C sites for carbohydrate modification.
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6• Zielinska, D.F., Gnad, F., Wisniewski, J.R., Mann, M., Precision mapping of an in vivo n-glycoproteome reveals rigid topological and sequence constraints. Cell 141 (2010), 897–907 Describes the use of ‘filter aided sample preparation’ to map 6367 N-glycosylation sites on 2352 proteins in mouse tissue and blood plasma. Besides the consensus N-X-S/T (X≠P) sequon for N-glycosylation, the study also shows rare N-X-C sites for carbohydrate modification.
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Comprehensive analysis of protein glycosylation by solid-phase extraction of n-linked glycans and glycosite-containing peptides
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7 Sun, S., Shah, P., Eshghi, S.T., Yang, W., Trikannad, N., Yang, S., Chen, L., Aiyetan, P., Hoti, N., Zhang, Z., Chan, D.W., et al. Comprehensive analysis of protein glycosylation by solid-phase extraction of n-linked glycans and glycosite-containing peptides. Nat Biotechnol 34 (2016), 84–88.
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8 Rakus, J.F., Mahal, L.K., New technologies for glycomic analysis: toward a systematic understanding of the glycome. Annu Rev Anal Chem 4 (2011), 367–392.
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N. Taniguchi T. Endo G.W. Hart PH. Seeberger C.-H. Wong Springer Japan Japan
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13 Guo, H., Pierce, J.M., Transcriptional regulation of glycan expression. Taniguchi, N., Endo, T., Hart, G.W., Seeberger, PH., Wong, C.-H., (eds.) Glycoscience: Biology and Medicine, 2015, Springer Japan, Japan, 1568.
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14 Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T.E., Handbook of Glycosyltransferases and Related Genes. 2014, Springer, Tokyo, Japan.
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Xbp1s links the unfolded protein response to the molecular architecture of mature n-glycans
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An example of how transcription factor activity can change the patter of N-linked glycosylation.
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15• Dewal, M.B., DiChiara, A.S., Antonopoulos, A., Taylor, R.J., Harmon, C.J., Haslam, S.M., Dell, A., Shoulders, M.D., Xbp1s links the unfolded protein response to the molecular architecture of mature n-glycans. Chem Biol 22 (2015), 1301–1312 An example of how transcription factor activity can change the patter of N-linked glycosylation.
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The transcription factor ATF2 promotes melanoma metastasis by suppressing protein fucosylation
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16 Lau, E., Feng, Y., Claps, G., Fukuda, M.N., Perlina, A., Donn, D., Jilaveanu, L., Kluger, H., Freeze, H.H., Ronai, Z.A., The transcription factor ATF2 promotes melanoma metastasis by suppressing protein fucosylation. Sci Signal, 8, 2015, ra124.
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Regulation of glycan structures in murine embryonic stem cells: combined transcript profiling of glycan-related genes and glycan structural analysis
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Maps glycosylation related transcript levels to corresponding glycan structures. Concludes that glycan structure changes generally (but not always) correlate with alterations in glycogene transcript abundance.
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