Specific glycosylation of membrane proteins in epithelial ovarian cancer cell lines: Glycan structures reflect gene expression and DNA methylation status

Molecular and Cellular Proteomics

Volumn 13, Issue 9, 2014, Pages 2213-2232

  Anugraham, Merrina ^a   Jacob, Francis ^b,c   Nixdorf, Sheri ^c   Everest Dass, Arun Vijay ^a   Heinzelmann Schwarz, Viola ^b,c   Packer, Nicolle H ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY WOMEN S HOSPITAL   (Switzerland)

^c UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AZACITIDINE; GLYCAN; MEMBRANE PROTEIN; N ACETYLGLUCOSAMINE; GLYCOSYLTRANSFERASE; POLYSACCHARIDE;

A2780 CELL LINE; ARTICLE; CANCER MORTALITY; CELL MEMBRANE; CELL STRUCTURE; DNA METHYLATION; ELECTROSPRAY MASS SPECTROMETRY; EPIGENETICS; EPITHELIUM CELL; FEMALE; GENE EXPRESSION; GENE SYNTHESIS; GLYCAN STRUCTURE; HUMAN; HUMAN CELL; IGROV1 CELL LINE; LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; OVARIAN CANCER CELL LINE; OVARY CARCINOMA; OVCAR 3 CELL LINE; PROTEIN GLYCOSYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKOV 3 CELL LINE; CELL LINE; GENE EXPRESSION REGULATION; GENETICS; GLYCOSYLATION; METABOLISM; OVARY TUMOR; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE;

CELL LINE; CELL LINE, TUMOR; CHROMATOGRAPHY, LIQUID; DNA METHYLATION; EPITHELIAL CELLS; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GLYCOSYLATION; GLYCOSYLTRANSFERASES; HUMANS; MEMBRANE PROTEINS; OVARIAN NEOPLASMS; POLYSACCHARIDES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 84907200792     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.M113.037085     Document Type: Article

References (86)

1. Jemal, A., Siegel, R., Xu, J., and Ward, E. (2010) Cancer statistics, 2010. CA Cancer J. Clin. 60, 277-300
2. Vathipadiekal, V., Saxena, D., Mok, S. C., Hauschka, P. V., Ozbun, L., and Birrer, M. J. (2012) Identification of a potential ovarian cancer stem cell gene expression profile from advanced stage papillary serous ovarian cancer. PLoS One 7, e29079
3. Berkenblit, A., and Cannistra, S. A. (2005) Advances in the management of epithelial ovarian cancer. J. Reprod. Med. 50, 426-438
4. Sasaroli, D., Coukos, G., and Scholler, N. (2009) Beyond CA125: the coming of age of ovarian cancer biomarkers. Are we there yet? Biomark. Med. 3, 275-288
5. Meyer, T., and Rustin, G. J. (2000) Role of tumour markers in monitoring epithelial ovarian cancer. Br. J. Cancer 82, 1535-1538
6. Jacob, F., Meier, M., Caduff, R., Goldstein, D., Pochechueva, T., Hacker, N., Fink, D., and Heinzelmann-Schwarz, V. (2011) No benefit from combining HE4 and CA125 as ovarian tumor markers in a clinical setting. Gynecol. Oncol. 121, 487-491
7. Paulson, J. C., and Colley, K. J. (1989) Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation. J. Biol. Chem. 264, 17615-17618
8. Taylor, A. D., Hancock, W. S., Hincapie, M., Taniguchi, N., and Hanash, S. M. (2009) Towards an integrated proteomic and glycomic approach to finding cancer biomarkers. Genome Med. 1, 57
9. Dube, D. H., and Bertozzi, C. R. (2005) Glycans in cancer and inflammation-potential for therapeutics and diagnostics. Nat. Rev. Drug Discov. 4, 477-488
10. Hakomori, S. (1996) Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism. Cancer Res. 56, 5309-5318
11. Yamamoto, H., Swoger, J., Greene, S., Saito, T., Hurh, J., Sweeley, C., Leestma, J., Mkrdichian, E., Cerullo, L., Nishikawa, A., Ihara, Y., Taniguchi, N., and Moskal, J. R. (2000) Beta1,6-N-acetylglucosamine-bearing N-glycans in human gliomas: implications for a role in regulating invasivity. Cancer Res. 60, 134-142
12. Couldrey, C., and Green, J. E. (2000) Metastases: the glycan connection. Breast Cancer Res. 2, 321-323
13. Taniguchi, N., Ihara, S., Saito, T., Miyoshi, E., Ikeda, Y., and Honke, K. (2001) Implication of GnT-V in cancer metastasis: a glycomic approach for identification of a target protein and its unique function as an angiogenic cofactor. Glycoconj. J. 18, 859-865
14. Demetriou, M., Nabi, I. R., Coppolino, M., Dedhar, S., and Dennis, J. W. (1995) Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V. J. Cell Biol. 130, 383-392
15. Mechref, Y., Hu, Y., Garcia, A., Zhou, S., Desantos-Garcia, J. L., and Hussein, A. (2012) Defining putative glycan cancer biomarkers by MS. Bioanalysis 4, 2457-2469
16. Harvey, D. J. (2000) Collision-induced fragmentation of underivatized Nlinked carbohydrates ionized by electrospray. J. Mass Spectrom. 35, 1178-1190
17. Hayes, C. A., Karlsson, N. G., Struwe, W. B., Lisacek, F., Rudd, P. M., Packer, N. H., and Campbell, M. P. (2011) UniCarb-DB: a database resource for glycomic discovery. Bioinformatics 27, 1343-1344
18. Cooper, C. A., Gasteiger, E., and Packer, N. H. (2001) GlycoMod-a software tool for determining glycosylation compositions from mass spectrometric data. Proteomics 1, 340-349
19. Ceroni, A., Maass, K., Geyer, H., Geyer, R., Dell, A., and Haslam, S. M. (2008) GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans. J. Proteome Res. 7, 1650-1659
20. Maxwell, E., Tan, Y., Tan, Y., Hu, H., Benson, G., Aizikov, K., Conley, S., Staples, G. O., Slysz, G. W., Smith, R. D., and Zaia, J. (2012) GlycReSoft: a software package for automated recognition of glycans from LC/MS data. PLoS One 7, e45474
21. Yu, C. Y., Mayampurath, A., Hu, Y., Zhou, S., Mechref, Y., and Tang, H. (2013) Automated annotation and quantification of glycans using liquid chromatography-mass spectrometry. Bioinformatics 29, 1706-1707
22. Abbott, K. L., Lim, J. M., Wells, L., Benigno, B. B., McDonald, J. F., and Pierce, M. (2010) Identification of candidate biomarkers with cancerspecific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics 10, 470-481
23. Jacob, F., Goldstein, D. R., Bovin, N. V., Pochechueva, T., Spengler, M., Caduff, R., Fink, D., Vuskovic, M. I., Huflejt, M. E., and Heinzelmann-Schwarz, V. (2012) Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array. Int. J. Cancer 130, 138-146
24. Alley, W. R., Jr., Vasseur, J. A., Goetz, J. A., Svoboda, M., Mann, B. F., Matei, D. E., Menning, N., Hussein, A., Mechref, Y., and Novotny, M. V. (2012) N-linked glycan structures and their expressions change in the blood sera of ovarian cancer patients. J. Proteome Res. 11, 2282-2300
25. Shetty, V., Hafner, J., Shah, P., Nickens, Z., and Philip, R. (2012) Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics. Clin. Proteomics 9, 10
26. Colombo, N., Peiretti, M., Parma, G., Lapresa, M., Mancari, R., Carinelli, S., Sessa, C., Castiglione, M., and Group, E. G. W. (2010) Newly diagnosed and relapsed epithelial ovarian carcinoma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann. Oncol. 5, v23-30
27. Schiess, R., Wollscheid, B., and Aebersold, R. (2009) Targeted proteomic strategy for clinical biomarker discovery. Mol. Oncol. 3, 33-44
28. Kim, Y. S., and Deng, G. (2008) Aberrant expression of carbohydrate antigens in cancer: the role of genetic and epigenetic regulation. Gastroenterology 135, 305-309
29. Zoldos, V., Horvat, T., Novokmet, M., Cuenin, C., Muzinic, A., Pucic, M., Huffman, J. E., Gornik, O., Polasek, O., Campbell, H., Hayward, C., Wright, A. F., Rudan, I., Owen, K., McCarthy, M. I., Herceg, Z., and Lauc, G. (2012) Epigenetic silencing of HNF1A associates with changes in the composition of the human plasma N-glycome. Epigenetics 7, 164-172
30. Saldova, R., Dempsey, E., Perez-Garay, M., Marino, K., Watson, J. A., Blanco-Fernandez, A., Struwe, W. B., Harvey, D. J., Madden, S. F., Peracaula, R., McCann, A., and Rudd, P. M. (2011) 5-AZA-2-deoxycytidine induced demethylation influences N-glycosylation of secreted glycoproteins in ovarian cancer. Epigenetics 6, 1362-1372
31. Tsao, S. W., Mok, S. C., Fey, E. G., Fletcher, J. A., Wan, T. S., Chew, E. C., Muto, M. G., Knapp, R. C., and Berkowitz, R. S. (1995) Characterization of human ovarian surface epithelial cells immortalized by human papilloma viral oncogenes (HPV-E6E7 ORFs). Exp. Cell Res. 218, 499-507
32. Lee, A., Kolarich, D., Haynes, P. A., Jensen, P. H., Baker, M. S., and Packer, N. H. (2009) Rat liver membrane glycoproteome: enrichment by phase partitioning and glycoprotein capture. J. Proteome Res. 8, 770-781
33. Jensen, P. H., Karlsson, N. G., Kolarich, D., and Packer, N. H. (2012) Structural analysis of N-and O-glycans released from glycoproteins. Nat. Protoc. 7, 1299-1310
34. Estrella, R. P., Whitelock, J. M., Packer, N. H., and Karlsson, N. G. (2010) The glycosylation of human synovial lubricin: implications for its role in inflammation. Biochem. J. 429, 359-367
35. Issa, S., Moran, A. P., Ustinov, S. N., Lin, J. H., Ligtenberg, A. J., and Karlsson, N. G. (2010) O-linked oligosaccharides from salivary agglutinin: Helicobacter pylori binding sialyl-Lewis x and Lewis b are terminating moieties on hyperfucosylated oligo-N-acetyllactosamine. Glycobiology 20, 1046-1057
36. Karlsson, N. G., and Thomsson, K. A. (2009) Salivary MUC7 is a major carrier of blood group I type O-linked oligosaccharides serving as the scaffold for sialyl Lewis x. Glycobiology 19, 288-300
37. Everest-Dass, A. V., Jin, D., Thaysen-Andersen, M., Nevalainen, H., Kolarich, D., and Packer, N. H. (2012) Comparative structural analysis of the glycosylation of salivary and buccal cell proteins: innate protection against infection by C. albicans. Glycobiology
38. Nakano, M., Saldanha, R., Gobel, A., Kavallaris, M., and Packer, N. H. (2011) Identification of glycan structure alterations on cell membrane proteins in desoxyepothilone B resistant leukemia cells. Mol. Cell. Proteomics 10, M111 009001
39. Everest-Dass, A. V., Kolarich, D., Campbell, M. P., and Packer, N. H. (2013) Tandem mass spectra of glycan substructures enable the multistage mass spectrometric identification of determinants on oligosaccharides. Rapid Commun. Mass. Spectrom. 27, 931-939
40. Everest-Dass, A. V., Abrahams, J. L., Kolarich, D., Packer, N. H., and Campbell, M. P. (2013) Structural feature ions for distinguishing N-and O-linked glycan isomers by LC-ESI-IT MS/MS. J. Am. Soc. Mass Spectrom 24, 895-906
41. Harvey, D. J. (2005) Fragmentation of negative ions from carbohydrates: part 1. fragmentation of hybrid and complex N-linked glycans. J. Am. Soc. Mass. Spectrom 16, 647-659
42. Stanley, P., Schachter, H., and Taniguchi, N. (2009) N-Glycans. In: Varki, A., Cummings, R. D., Esko, J. D., Freeze, H. H., Stanley, P., Bertozzi, C. R., Hart, G. W., and Etzler, M. E., eds. Essentials of Glycobiology, 2nd Ed., Cold Spring Harbor (NY)
43. Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., and Wittwer, C. T. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55, 611-622
44. Jacob, F., Guertler, R., Naim, S., Nixdorf, S., Fedier, A., Hacker, N. F., and Heinzelmann-Schwarz, V. (2013) Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines. PLoS One 8, e59180
45. Arvidsson, S., Kwasniewski, M., Riano-Pachon, D. M., and Mueller-Roeber, B. (2008) QuantPrime-a flexible tool for reliable high-throughput primer design for quantitative PCR. BMC Bioinformatics 9, 465
46. Kent, W. J., Sugnet, C. W., Furey, T. S., Roskin, K. M., Pringle, T. H., Zahler, A. M., and Haussler, D. (2002) The human genome browser at UCSC. Genome Res. 12, 996-1006
47. Pabst, M., Bondili, J. S., Stadlmann, J., Mach, L., and Altmann, F. (2007) Mass retention time structure: a strategy for the analysis of Nglycans by carbon LC-ESI-MS and its application to fibrin N-glycans. Anal. Chem. 79, 5051-5057
48. Harvey, D. J., Crispin, M., Scanlan, C., Singer, B. B., Lucka, L., Chang, V. T., Radcliffe, C. M., Thobhani, S., Yuen, C. T., and Rudd, P. M. (2008) Differentiation between isomeric triantennary N-linked glycans by negative ion tandem mass spectrometry and confirmation of glycans containing galactose attached to the bisecting (beta1-4-GlcNAc) residue in N-glycans from IgG. Rapid Commun. Mass. Spectrom. 22, 1047-1052
49. Rodriguez-Paredes, M., and Esteller, M. (2011) Cancer epigenetics reaches mainstream oncology. Nat. Med. 17, 330-339
50. Kui Wong, N., Easton, R. L., Panico, M., Sutton-Smith, M., Morrison, J. C., Lattanzio, F. A., Morris, H. R., Clark, G. F., Dell, A., and Patankar, M. S. (2003) Characterization of the oligosaccharides associated with the human ovarian tumor marker CA125. J. Biol. Chem. 278, 28619-28634
51. Abbott, K. L., Nairn, A. V., Hall, E. M., Horton, M. B., McDonald, J. F., Moremen, K. W., Dinulescu, D. M., and Pierce, M. (2008) Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics 8, 3210-3220
52. Schachter, H. (1986) Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides. Adv. Exp. Med. Biol. 205, 53-85
53. Takahashi, M., Kuroki, Y., Ohtsubo, K., and Taniguchi, N. (2009) Core fucose and bisecting GlcNAc, the direct modifiers of the N-glycan core: their functions and target proteins. Carbohydr. Res. 344, 1387-1390
54. Lau, K. S., Partridge, E. A., Grigorian, A., Silvescu, C. I., Reinhold, V. N., Demetriou, M., and Dennis, J. W. (2007) Complex N-glycan number and degree of branching cooperate to regulate cell proliferation and differentiation. Cell 129, 123-134
55. Pinho, S. S., Oliveira, P., Cabral, J., Carvalho, S., Huntsman, D., Gartner, F., Seruca, R., Reis, C. A., and Oliveira, C. (2012) Loss and recovery of Mgat3 and GnT-III Mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-mesenchymal-epithelial transitions. PLoS One 7, e33191
56. Wilson, B. G., and Roberts, C. W. (2011) SWI/SNF nucleosome remodellers and cancer. Nat. Rev. Cancer 11, 481-492
57. Kelly, T. K., Liu, Y., Lay, F. D., Liang, G., Berman, B. P., and Jones, P. A. (2012) Genome-wide mapping of nucleosome positioning and DNA methylation within individual DNA molecules. Genome Res. 22, 2497-2506
58. Fwu, P. T., Wang, P. H., Tung, C. K., and Dong, C. Y. (2005) Effects of index-mismatch-induced spherical aberration in pump-probe microscopic image formation. Appl. Opt 44, 4220-4227
59. Tsuji, S. (1996) Molecular cloning and functional analysis of sialyltransferases. J. Biochem. 120, 1-13
60. Dall'Olio, F., Chiricolo, M., and Lau, J. T. (1999) Differential expression of the hepatic transcript of beta-galactoside alpha2,6-sialyltransferase in human colon cancer cell lines. Int. J. Cancer 81, 243-247
61. Recchi, M. A., Hebbar, M., Hornez, L., Harduin-Lepers, A., Peyrat, J. P., and Delannoy, P. (1998) Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in human breast cancer. Cancer Res. 58, 4066-4070
62. Wang, P. H., Lee, W. L., Lee, Y. R., Juang, C. M., Chen, Y. J., Chao, H. T., Tsai, Y. C., and Yuan, C. C. (2003) Enhanced expression of alpha 2,6-sialyltransferase ST6Gal I in cervical squamous cell carcinoma. Gynecol. Oncol. 89, 395-401
63. Pousset, D., Piller, V., Bureaud, N., Monsigny, M., and Piller, F. (1997) Increased alpha2,6 sialylation of N-glycans in a transgenic mouse model of hepatocellular carcinoma. Cancer Res. 57, 4249-4256
64. Saldova, R., Royle, L., Radcliffe, C. M., Abd Hamid, U. M., Evans, R., Arnold, J. N., Banks, R. E., Hutson, R., Harvey, D. J., Antrobus, R., Petrescu, S. M., Dwek, R. A., and Rudd, P. M. (2007) Ovarian cancer is associated with changes in glycosylation in both acute-phase proteins and IgG. Glycobiology 17, 1344-1356
65. Christie, D. R., Shaikh, F. M., Lucas, J. A. t., Lucas, J. A., 3rd, and Bellis, S. L. (2008) ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function. J. Ovarian Res. 1, 3
66. Harduin-Lepers, A., Recchi, M. A., and Delannoy, P. (1995) 1994, the year of sialyltransferases. Glycobiology 5, 741-758
67. Kitagawa, H., and Paulson, J. C. (1993) Cloning and expression of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 194, 375-382
68. Kitagawa, H., and Paulson, J. C. (1994) Cloning of a novel alpha 2,3-sialyltransferase that sialylates glycoprotein and glycolipid carbohydrate groups. J. Biol. Chem. 269, 1394-1401
69. Ishii, A., Ohta, M., Watanabe, Y., Matsuda, K., Ishiyama, K., Sakoe, K., Nakamura, M., Inokuchi, J., Sanai, Y., and Saito, M. (1998) Expression cloning and functional characterization of human cDNA for ganglioside GM3 synthase. J. Biol. Chem. 273, 31652-31655
70. Harduin-Lepers, A., Mollicone, R., Delannoy, P., and Oriol, R. (2005) The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. Glycobiology 15, 805-817
71. Kawar, Z. S., Haslam, S. M., Morris, H. R., Dell, A., and Cummings, R. D. (2005) Novel poly-GalNAcbeta1-4GlcNAc (LacdiNAc) and fucosylated poly-LacdiNAc N-glycans from mammalian cells expressing beta1,4-N-acetylgalactosaminyltransferase and alpha1,3-fucosyltransferase. J. Biol. Chem. 280, 12810-12819
72. Wuhrer, M., Koeleman, C. A., Deelder, A. M., and Hokke, C. H. (2006) Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni. FEBS J. 273, 347-361
73. Yu, S. Y., Chang, L. Y., Cheng, C. W., Chou, C. C., Fukuda, M. N., and Khoo, K. H. (2013) Priming mass spectrometry-based sulfoglycomic mapping for identification of terminal sulfated lacdiNAc glycotope. Glycoconj. J. 30, 183-194
74. Saarinen, J., Welgus, H. G., Flizar, C. A., Kalkkinen, N., and Helin, J. (1999) N-glycan structures of matrix metalloproteinase-1 derived from human fibroblasts and from HT-1080 fibrosarcoma cells. Eur. J. Biochem. 259, 829-840
75. Smith, P. L., and Baenziger, J. U. (1992) Molecular basis of recognition by the glycoprotein hormone-specific N-acetylgalactosamine-transferase. Proc. Natl. Acad. Sci. U.S.A. 89, 329-333
76. Dell, A., Morris, H. R., Easton, R. L., Panico, M., Patankar, M., Oehniger, S., Koistinen, R., Koistinen, H., Seppala, M., and Clark, G. F. (1995) Structural analysis of the oligosaccharides derived from glycodelin, a human glycoprotein with potent immunosuppressive and contraceptive activities. J. Biol. Chem. 270, 24116-24126
77. Woodworth, A., Fiete, D., and Baenziger, J. U. (2002) Spatial and temporal regulation of tenascin-R glycosylation in the cerebellum. J. Biol. Chem. 277, 50941-50947
78. Sato, T., Gotoh, M., Kiyohara, K., Kameyama, A., Kubota, T., Kikuchi, N., Ishizuka, Y., Iwasaki, H., Togayachi, A., Kudo, T., Ohkura, T., Nakanishi, H., and Narimatsu, H. (2003) Molecular cloning and characterization of a novel human beta 1,4-N-acetylgalactosaminyltransferase, beta 4GalNAc-T3, responsible for the synthesis of N,N-diacetyllactosediamine, galNAc beta 1-4GlcNAc. J. Biol. Chem. 278, 47534-47544
79. Gotoh, M., Sato, T., Kiyohara, K., Kameyama, A., Kikuchi, N., Kwon, Y. D., Ishizuka, Y., Iwai, T., Nakanishi, H., and Narimatsu, H. (2004) Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N-diacetyllactosediamine. FEBS Lett. 562, 134-140
80. Chan, A. L., Morris, H. R., Panico, M., Etienne, A. T., Rogers, M. E., Gaffney, P., Creighton-Kempsford, L., and Dell, A. (1991) A novel sialylated Nacetylgalactosamine-containing oligosaccharide is the major complextype structure present in Bowes melanoma tissue plasminogen activator. Glycobiology 1, 173-185
81. Peracaula, R., Royle, L., Tabares, G., Mallorqui-Fernandez, G., Barrabes, S., Harvey, D. J., Dwek, R. A., Rudd, P. M., and de Llorens, R. (2003) Glycosylation of human pancreatic ribonuclease: differences between normal and tumor states. Glycobiology 13, 227-244
82. Machado, E., Kandzia, S., Carilho, R., Altevogt, P., Conradt, H. S., and Costa, J. (2011) N-Glycosylation of total cellular glycoproteins from the human ovarian carcinoma SKOV3 cell line and of recombinantly expressed human erythropoietin. Glycobiology 21, 376-386
83. Goetz, J. A., Mechref, Y., Kang, P., Jeng, M. H., and Novotny, M. V. (2009) Glycomic profiling of invasive and non-invasive breast cancer cells. Glycoconj. J. 26, 117-131
84. Newsom-Davis, T. E., Wang, D., Steinman, L., Chen, P. F., Wang, L. X., Simon, A. K., and Screaton, G. R. (2009) Enhanced immune recognition of cryptic glycan markers in human tumors. Cancer Res. 69, 2018-2025
85. Johns, T. G., Mellman, I., Cartwright, G. A., Ritter, G., Old, L. J., Burgess, A. W., and Scott, A. M. (2005) The antitumor monoclonal antibody 806 recognizes a high-mannose form of the EGF receptor that reaches the cell surface when cells over-express the receptor. FASEB J. 19, 780-782
86. Yap, T. A., Carden, C. P., and Kaye, S. B. (2009) Beyond chemotherapy: targeted therapies in ovarian cancer. Nat. Rev. Cancer 9, 167-181