A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells

Stem Cell Reports

Volumn 7, Issue 3, 2016, Pages 508-517

  Howden, Sara E ^a,b   McColl, Bradley ^a   Glaser, Astrid ^a,b   Vadolas, Jim ^a,b   Petrou, Steven ^c   Little, Melissa H ^a,b   Elefanty, Andrew G ^a,b,d   Stanley, Edouard G ^a,b,d  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c UNIVERSITY OF MELBOURNE   (Australia)

^d MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; BACTERIAL RNA; CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; DNA METHYLTRANSFERASE 3B; GEMININ; GENOMIC DNA; MESSENGER RNA; NUCLEASE; UNCLASSIFIED DRUG;

ALLELE; AMPLICON; ARTICLE; CELL CYCLE G1 PHASE; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA REPAIR; GENE TARGETING; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; INDEL MUTATION; INDUCED PLURIPOTENT STEM CELL; MICROBIAL DEGRADATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; START CODON; STREPTOCOCCUS PYOGENES; TRANSIENT EXPRESSION; WESTERN BLOTTING; CELL LINE; CRISPR CAS SYSTEM; CYTOLOGY; DNA SEQUENCE; FIBROBLAST; GENE EXPRESSION; GENETICS; METABOLISM; NUCLEAR REPROGRAMMING; NUCLEOTIDE SEQUENCE; PLURIPOTENT STEM CELL; REPORTER GENE; TARGETED GENE REPAIR;

BASE SEQUENCE; CELL LINE; CELLULAR REPROGRAMMING; CRISPR-CAS SYSTEMS; DNA END-JOINING REPAIR; FIBROBLASTS; GEMININ; GENE EXPRESSION; GENE KNOCK-IN TECHNIQUES; GENES, REPORTER; HUMANS; INDEL MUTATION; PLURIPOTENT STEM CELLS; SEQUENCE ANALYSIS, DNA; TARGETED GENE REPAIR; TRANSFECTION;

EID: 84990042035     PISSN: 22136711     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.stemcr.2016.07.001     Document Type: Article

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