Chromatin-binding regions of EBNA1 protein facilitate the enhanced transfection of Epstein-Barr virus-based vectors

Human Gene Therapy

Volumn 17, Issue 8, 2006, Pages 833-844

  Howden, Sara E ^a   Wardan, Hady ^a   Voullaire, Lucille ^a   McLenachan, Samuel ^a   Williamson, Robert ^a   Ioannou, Panos ^a   Vadolas, Jim ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EPSTEIN BARR VIRUS NUCLEAR ANTIGEN 1; MESSENGER RNA; UNCLASSIFIED DRUG; VIRUS ANTIGEN; EBV ENCODED NUCLEAR ANTIGEN 1; EBV-ENCODED NUCLEAR ANTIGEN 1; EPSTEIN BARR VIRUS ANTIGEN; VIRUS RNA;

ANTIGEN EXPRESSION; ARTICLE; CHROMATIN BINDING REGION; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA BINDING DOMAIN; EPSTEIN BARR VIRUS VECTOR; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MODIFICATION; VIRUS VECTOR; ACTIVE TRANSPORT; CELL NUCLEUS; CELL STRAIN K 562; CHROMATIN; EPSTEIN BARR VIRUS; GENE THERAPY; GENE VECTOR; GENETIC DISORDER; GENETICS; HELA CELL; METABOLISM; METHODOLOGY; PROTEIN TERTIARY STRUCTURE;

HUMAN HERPESVIRUS 4; MAMMALIA;

ACTIVE TRANSPORT, CELL NUCLEUS; CELL NUCLEUS; CHROMATIN; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; GENE THERAPY; GENETIC DISEASES, INBORN; GENETIC VECTORS; HELA CELLS; HERPESVIRUS 4, HUMAN; HUMANS; K562 CELLS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RNA, MESSENGER; RNA, VIRAL; TRANSFECTION;

EID: 33750598248     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.2006.17.833     Document Type: Article

References (31)

1. BELT, P.B., JONGMANS, W., DE WIT, J., HOEIJMAKERS, J.H., VAN DE PUTTE, P., and BACKENDORF, C. (1991). Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT2) using an Epstein-Barr virus-derived cDNA expression vector. Nucleic Acids Res. 19, 4861-4866.
2. CARTIER, D., and RESZKA, R. (2002). Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems. Gene Ther. 9, 157-167.
3. CHAN, C.K., and JANS, D.A. (2002). Using nuclear targeting signals to enhance non-viral gene transfer. Immunol. Cell. Biol. 80, 119-130.
4. DOWTY, M.E., WILLIAMS, P., ZHANG, G., HAGSTROM, J.E., and WOLFF, J.A. (1995). Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc. Natl. Acad. Sci. U.S.A. 92, 4572-4576.
5. ESCRIOU, V., CARRIERE, M., BUSSONE, F., WILS, P., and SCHERMAN, D. (2001). Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene therapy. J. Gene Med. 3, 179-187.
6. FISCHER, N., KREMMER, E., LAUTSCHAM, G., MULLER-LANTZSCH, N., and GRÄSSER, F.A. (1997). Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin α2. J. Biol. Chem. 272, 3999-4005.
7. GÖRLICH, D., and MATTAJ, I.W. (1996) Nucleocytoplasmic transport. Science 271, 1513-1518.
8. KANEDA, Y., SAEHI, Y., NAKABAYASI, M., ZHOU, W., KANEDA, M., and MORISHITA, R. (2000). Enhancement of transgene expression by cotransfection of oriP plasmid with EBNA-1 expression vector. Hum. Gene Ther. 11, 471-479.
9. LANGLE-ROUAULT, F., PATZEL, V., BENAVENTE, A., TAILEZ, M., SILVERSTRE, N., BOMPARD, A., JACOBS, E., and RITTNER, K. (1998). Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: Implications of the nuclear import of plasmids. J. Virol. 72, 6181-6185.
10. MACKEY, D., and SUGDEN, B. (1999). The linking regions of EBNA1 are essential for its support of replication and transcription. Mol. Cell. Biol. 19, 3349-3359.
11. MARECHAL, V., DEHEE, A., CHIKHI-BRACHET, R., PIOLOT, T., COPPEY-MOISAN, M., and NICOLAS, J.C. (1999). Mapping EBNA-1 domains involved in binding to metaphase chromosomes. J. Virol. 73, 4385-4392.
12. MAZDA, O. (2002). Improvement of nonviral gene therapy by Epstein-Barr virus (EBV)-based plasmid vectors. Curr. Gene Ther. 2, 379-392.
13. MAZDA, O., ATOH, E., YASUTOMI, K., and IMANISHI, J. (1997). Extremely efficient gene transfection into lympho-haematopoietic cell lines by Epstein-Barr virus-based vectors. J. Immunol. Methods 204, 143-151.
14. MIDDLETON, T., and SUGDEN, B. (1994). Retention of plasmid DNA in mammalian cells is enhanced by binding of the Epstein-Barr virus replication protein EBNA1. J. Virol. 68, 4067-4071.
15. MIRZAYANS, R., AUBIN, R.A., and PATERSON, M.C. (1992). Differential expression and stability of foreign genes introduced into human fibroblasts by nuclear versus cytoplasmic microinjection. Mutat. Res. 281, 115-122.
16. ORFORD, M., JAMSAI, D., MCLENACHAN, S., and IOANNOU, P. (2004). Modification of human bacterial artificial chromosome clones for functional studies and therapeutic applications. Methods Mol. Biol. 240, 243-266.
17. PONSAERTS, P., BROWN, J.P., VAN DEN PLAS, D., VAN DEN EEDEN, L., VAN BOCKSTAELE, D.R., JORENS, J.P., VAN TANDELOO, V.F.I., MERREGAERT, J., SINGH, P.B., and BERNEMAN, Z.N. (2004). Messenger RNA electroporation is highly efficient in mouse embryonic stem cells: Successful FLPe- and Cre-mediated recombination. Gene Ther. 11, 1606-1610.
18. RAMAGE, A., CLARK, A., SMITH, A.G., MOUNTFORD, P., and BURT, D.W. (1997). Improved EBV-based shuttle vector system: Dicistronic mRNA couples the synthesis of the Epstein-Barr nuclear antigen-1 protein to neomycin resistance. Gene 197, 83-89.
19. REISMAN, D., YATES, J., and SUGDEN, B. (1985) A putative origin of replication of plasmid derived from Epstein-Barr virus is composed of two cis-acting components. Mol. Cell. Biol. 5, 410-413.
20. SEARS, J., KOLMAN, J., WAHL, G.M., and AIYAR, A. (2003). Metaphase chromosome tethering is necessary for the DNA synthesis and maintenance of oriP plasmids but is insufficient for transcription activation by Epstein-Barr nuclear antigen 1. J. Virol. 77, 11767-11780.
21. SEARS, J., UJIHARA, M., WONG, S., OTT, C., MIDDELDORP, J., and AIYAR, A. (2004). The amino terminus of Epstein-Barr virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes. J. Virol. 78, 11487-11505.
22. SHIRE, K., CECCARELLI, D., AVOLIO-HUNTER, T., and FRAPPIER, L. (1999). EBP2, a human protein that interacts with sequences of the Epstein-Barr virus nuclear antigen 1 important for plasmid maintenance. J. Virol. 73, 2587-2595.
23. SUGDEN, B., and WARREN, N. (1988). Plasmid origin of replication of Epstein-Barr virus, oriP, does not limit replication in cis. Mol. Biol. Med. 5, 85-94.
24. SUGDEN, B., MARSH, K., and YATES, J. (1985). A vector that replicates as a plasmid and can be efficiently selected in B-lymphocytes transformed by Epstein-Barr virus. Mol. Cell. Biol. 5, 410-413.
25. TU, G., KIRCHMAIER, A., LIGGITT, D., LIU, Y., LIU, S., YU, W.H., HEATH, T.D., THOR, A., and DEBS, R.J. (2000). Non-replicating Epstein-Barr virus-based plasmids extend gene expression and can improve gene therapy in vivo. J. Biol. Chem. 275, 30408-30416.
26. VADOLAS, J., WARDAN, H., ORFORD, M., VOULLAIRE, L., ZAIBAK, F., WILLIAMSON, R., and IOANNOU, I. (2002). Development of sensitive fluorescent assays for embryonic and fetal hemoglobin inducers using the β-globin locus in erthropoietic cells. Blood 100, 4209-4215.
27. VAN TENDELOO, V.F.I., PONSAERTS, P., LARDON, F., NIJS, G., LENJOU, M., VAN BROECKHOVEN, C., VAN BOCKSTAELE, D.R., and BERNEMAN, Z.N. (2001). Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: Superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells. Blood 98, 49-56.
28. YATES, J., WARREN, N., REISMAN, D., and SUGDEN, B. (1984). A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells. Proc. Natl. Acad. Sci. U.S.A. 81, 3806-3810.
29. YATES, J., WARREN, N., and SUGDEN, B. (1985). Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature 313, 812-815.
30. YONEDA, Y. (1997). How proteins are transported from cytoplasm to the nucleus. J. Biochem. Tokyo 121, 811-817.
31. ZABNER, J., FASSENDER, A.J., MONINGER, T., POELLINGER, K.A., and WELSH, M.J. (1995). Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem. 270, 18997-19007.