Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 39, 2016, Pages 10848-10853

  Xu, Peng ^a,b   Qiao, Kangjian ^a   Ahn, Woo Suk ^a   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology   (United States)

^b UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

Author keywords

Advanced biofuels; Metabolic engineering; Oleaginous yeast; Oleochemicals; Synthetic biology

Indexed keywords

ACETYL COENZYME A; ACYL COENZYME A; ACYL COENZYME A DEHYDROGENASE; ADENOSINE TRIPHOSPHATE CITRATE SYNTHASE; ALDEHYDE DEHYDROGENASE; ALKANE; CARNITINE ACETYLTRANSFERASE; CHEMICAL COMPOUND; FATTY ACID; FATTY ACID ESTER; FATTY ACID SYNTHASE; FATTY ALCOHOL; FREE RADICAL; FUEL; GLUCOSE; OLEOCHEMICAL; PALMITOYL COENZYME A HYDROLASE; PYRUVATE DECARBOXYLASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRIDECANE; UNCLASSIFIED DRUG; ACYL CARRIER PROTEIN; BIOFUEL; ESTER;

ALCOHOL PRODUCTION; CATALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; CYTOPLASM; CYTOSOL; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; FATTY ACID OXIDATION; FERMENTATION; GLUCOSE INTAKE; HYDROLYSIS; LIPID COMPOSITION; LIPOGENESIS; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PEROXISOME; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; STARVATION; SYNTHESIS; YARROWIA LIPOLYTICA; CELL FRACTIONATION; METABOLIC ENGINEERING; METABOLISM; TRAFFIC AND TRANSPORT; YARROWIA;

ACYL CARRIER PROTEIN; ACYL COENZYME A; ALKANES; BIOFUELS; CYTOSOL; ESTERS; FATTY ACIDS; FATTY ALCOHOLS; METABOLIC ENGINEERING; SUBCELLULAR FRACTIONS; TRANSPORTATION; YARROWIA;

EID: 84989918349     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1607295113     Document Type: Article

References (41)

1. Knothe G (2010) Biodiesel and renewable diesel: A comparison. Pror Energy Combust Sci 36(3):364-373.
2. Xu P, et al. (2013) Modular optimization of multi-gene pathways for fatty acids production in E. Coli. Nat Commun 4:1409.
3. Xu P, Li L, Zhang F, Stephanopoulos G, Koffas M (2014) Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc Natl Acad Sci USA 111(31):11299-11304.
4. Runguphan W, Keasling JD (2014) Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals. Metab Eng 21:103-113.
5. de Jong BW, Shi S, Siewers V, Nielsen J (2014) Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway. Microb Cell Fact 13(1):39.
6. Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J (2014) Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters. Biotechnol Bioeng 111(9):1740-1747.
7. Tai YS, Xiong M, Zhang K (2015) Engineered biosynthesis of medium-chain esters in Escherichia coli. Metab Eng 27:20-28.
8. Blazeck J, Liu L, Knight R, Alper HS (2013) Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica. J Biotechnol 165(3-4):184-194.
9. Tai M, Stephanopoulos G (2013) Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metab Eng 15:1-9.
10. Qiao K, et al. (2015) Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica. Metab Eng 29:56-65.
11. Buijs NA, Zhou YJ, Siewers V, Nielsen J (2015) Long-chain alkane production by the yeast Saccharomyces cerevisiae. Biotechnol Bioeng 112(6):1275-1279.
12. Li-Beisson Y, et al. (2010) Acyl-lipid metabolism. Arabidopsis Book 8:e0133.
13. Mlícková K, et al. (2004) Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica. Appl Environ Microbiol 70(7):3918-3924.
14. Bates PD, Browse J (2012) The significance of different diacylgycerol synthesis pathways on plant oil composition and bioengineering. Front Plant Sci 3:147.
15. Hiltunen JK, et al. (2003) The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 27(1):35-64.
16. Knudsen J, Neergaard TB, Gaigg B, Jensen MV, Hansen JK (2000) Role of acyl-CoA binding protein in acyl-CoA metabolism and acyl-CoA-mediated cell signaling. J Nutr 130(Suppl 2S):294S-298S.
17. Kalscheuer R, Stölting T, Steinbüchel A (2006) Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152(Pt 9):2529-2536.
18. Schirmer A, Rude MA, Li X, Popova E, del Cardayre SB (2010) Microbial biosynthesis of alkanes. Science 329(5991):559-562.
19. Kallio P, Pásztor A, Thiel K, Akhtar MK, Jones PR (2014) An engineered pathway for the biosynthesis of renewable propane. Nat Commun 5:4731.
20. Rajakovich LJ, et al. (2015) Rapid reduction of the diferric-peroxyhemiacetal intermediate in aldehyde-deformylating oxygenase by a cyanobacterial ferredoxin: Evidence for a free-radical mechanism. J Am Chem Soc 137(36):11695-11709.
21. Swiegers JH, Dippenaar N, Pretorius IS, Bauer FF (2001) Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: Three carnitine acetyltransferases are essential in a carnitine-dependent strain. Yeast 18(7):585-595.
22. Strijbis K, Distel B (2010) Intracellular acetyl unit transport in fungal carbon metabolism. Eukaryot Cell 9(12):1809-1815.
23. Stehlik T, Sandrock B, Ast J, Freitag J (2014) Fungal peroxisomes as biosynthetic organelles. Curr Opin Microbiol 22:8-14.
24. Akhtar MK, Turner NJ, Jones PR (2013) Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. Proc Natl Acad Sci USA 110(1):87-92.
25. Pfleger BF, Gossing M, Nielsen J (2015) Metabolic engineering strategies for microbial synthesis of oleochemicals. Metab Eng 29:1-11.
26. Jenni S, et al. (2007) Structure of fungal fatty acid synthase and implications for iterative substrate shuttling. Science 316(5822):254-261.
27. Lomakin IB, Xiong Y, Steitz TA (2007) The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together. Cell 129(2):319-332.
28. Schweizer E, Hofmann J (2004) Microbial type I fatty acid synthases (FAS): Major players in a network of cellular FAS systems. Microbiol Mol Biol Rev 68(3):501-517.
29. Leber C, Da Silva NA (2014) Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids. Biotechnol Bioeng 111(2):347-358.
30. Fernandez-Moya R, Leber C, Cardenas J, Da Silva NA (2015) Functional replacement of the Saccharomyces cerevisiae fatty acid synthase with a bacterial type II system allows flexible product profiles. Biotechnol Bioeng 112(12):2618-2623.
31. Haushalter RW, et al. (2015) Development of an orthogonal fatty acid biosynthesis system in E. Coli for oleochemical production. Metab Eng 30:1-6.
32. Eriksen DT, HamediRad M, Yuan Y, Zhao H (2015) Orthogonal fatty acid biosynthetic pathway improves fatty acid ethyl ester production in Saccharomyces cerevisiae. ACS Synth Biol 4(7):808-814.
33. Yu A-Q, Juwono NK, Foo JL, Leong SS, Chang MW (2016) Metabolic engineering of Saccharomyces cerevisiae for the overproduction of short branched-chain fatty acids. Metab Eng 34:36-43.
34. Teo WS, Ling H, Yu AQ, Chang MW (2015) Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid short- and branched-chain alkyl esters biodiesel. Biotechnol Biofuels 8(1):177.
35. Liu A, Tan X, Yao L, Lu X (2013) Fatty alcohol production in engineered E. Coli expressing Marinobacter fatty acyl-CoA reductases. Appl Microbiol Biotechnol 97(15): 7061-7071.
36. Feng X, Lian J, Zhao H (2015) Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production. Metab Eng 27:10-19.
37. Lian J, Si T, Nair NU, Zhao H (2014) Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. Metab Eng 24:139-149.
38. Fournier P, et al. (1993) Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica. Proc Natl Acad Sci USA 90(11):4912-4916.
39. Vernis L, et al. (1997) An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica. Mol Cell Biol 17(4): 1995-2004.
40. Xu P, Vansiri A, Bhan N, Koffas MA (2012) ePathBrick: A synthetic biology platform for engineering metabolic pathways in E. Coli. ACS Synth Biol 1(7):256-266
41. Chen DC, Beckerich JM, Gaillardin C (1997) One-step transformation of the dimorphic yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 48(2):232-235.