Fatty alcohol production in engineered E. coli expressing Marinobacter fatty acyl-CoA reductases

Applied Microbiology and Biotechnology

Volumn 97, Issue 15, 2013, Pages 7061-7071

  Liu, Aiqiu ^a,b,c   Tan, Xiaoming ^a   Yao, Lun ^a,c   Lu, Xuefeng ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

^b SHANDONG UNIVERSITY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Escherichia coli; Fatty acyl CoA reductase; Fatty alcohol; Marinobacter aquaeolei VT8

Indexed keywords

ACYL-ACP THIOESTERASES; ENGINEERED ESCHERICHIA COLI; FATTY ACYL-COA REDUCTASE; FATTY ALCOHOLS; FERMENTATION CONDITIONS; HETEROLOGOUS EXPRESSION; MARINOBACTER; SUBSTRATE SPECIFICITY;

CARBON; CHAIN LENGTH; ENZYME ACTIVITY; ESCHERICHIA COLI; GENES;

ALCOHOLS;

ACYL COENZYME A DEHYDROGENASE; FATTY ACID; FATTY ALCOHOL;

ALCOHOL; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; HETEROGENEITY; METABOLISM; MICROBIAL COMMUNITY; MUTATION; OPTIMIZATION; SUBSTRATE;

ALCOHOL PRODUCTION; ARTICLE; BATCH FERMENTATION; BIOSYNTHESIS; CELL GROWTH; DNA SEQUENCE; ENZYME SPECIFICITY; ESCHERICHIA COLI; FATTY ALCOHOL PRODUCTION; GLUCOSE INTAKE; HETEROLOGOUS EXPRESSION; LIPID COMPOSITION; MARINOBACTER; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION;

ALDEHYDE OXIDOREDUCTASES; BASE SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI; FATTY ALCOHOLS; FERMENTATION; MARINOBACTER; PROTEIN ENGINEERING;

ESCHERICHIA COLI; MARINOBACTER; MARINOBACTER HYDROCARBONOCLASTICUS;

EID: 84880511769     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5027-2     Document Type: Article

References (38)

1. Black PN, DiRusso CC, Metzger AK, Heimert TL (1992) Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase. J Biol Chem 267:25513-25520
2. Carlsson AS, Yilmaz JL, Green AG, Stymne S, Hofvander P (2011) Replacing fossil oil with fresh oil - with what and for what? Eur J Lipid Sci Technol 113:812-831
3. Cheng JB (2004) Mammalian wax biosynthesis: I. Identification of two fatty acyl-coenzyme A reductases with different substrate specificities and tissue distributions. J Biol Chem 279:37789-37797
4. Cho H, Cronan JE Jr (1995) Defective export of a periplasmic enzyme disrupts regulation of fatty acid synthesis. J Biol Chem 270:4216-4219
5. Davis MS (2000) Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. J Biol Chem 275:28593-28598
6. Doan TTP, Carlsson AS, Hamberg M, Bülow L, Stymne S, Olsson P (2009) Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli. J Plant Physiol 166:787-796
7. Domergue F, Vishwanath SJ, Joubes J, Ono J, Lee JA, Bourdon M, Alhattab R, Lowe C, Pascal S, Lessire R, Rowland O (2010) Three Arabidopsis fatty acyl-Coenzyme A reductases, FAR1, FAR4, and FAR5, generate primary fatty alcohols associated with suberin deposition. Plant Physiol 153:1539-1554
8. Duan Y, Zhu Z, Cai K, Tan X, Lu X (2011) De novo biosynthesis of biodiesel by Escherichia coli in optimized fed-batch cultivation. PLoS One 6:e20265
9. Fortman JL, Chhabra S, Mukhopadhyay A, Chou H, Lee TS, Steen E, Keasling JD (2008) Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol 26:375-381
10. Gervajio GC (2005) Fatty acids and derivatives from coconut oil. Bailey's Industrial Oil and Fat Products. John Wiley & Sons, Inc
11. Hofvander P, Doan TTP, Hamberg M (2011) A prokaryotic acyl-CoA reductase performing reduction of fatty acyl-CoA to fatty alcohol. FEBS Lett 585:3538-3543
12. Jiang P, Cronan JE Jr (1994) Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J Bacteriol 176:2814-2821
13. Kaczmarzyk D, Fulda M (2010) Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling. Plant Physiol 152:1598-1610
14. Kalscheuer R, Stolting T, Steinbuchel A (2006) Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152:2529-2536
15. Kameda K, Nunn WD (1981) Purification and characterization of acyl coenzyme A synthetase from Escherichia coli. J Biol Chem 256:5702-5707
16. Kanya TCS, Rao LJ, Sastry MCS (2007) Characterization of wax esters, free fatty alcohols and free fatty acids of crude wax from sunflower seed oil refineries. Food Chem 101:1552-1557
17. Li M, Zhang X, Agrawal A, San K-Y (2012) Effect of acetate formation pathway and long chain fatty acid CoA-ligase on the free fatty acid production in E. coli expressing acy-ACP thioesterase from Ricinus communis. Metab Eng 14:380-387
18. Liu T, Vora H, Khosla C (2010) Quantitative analysis and engineering of fatty acid biosynthesis in E. coli. Metab Eng 12:378-386
19. Liu XSJ, Curtiss R (2011) Fatty acid production in genetically modified cyanobacteria. Proc Natl Acad Sci USA 6899-6904
20. Lu X, Vora H, Khosla C (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab Eng 10:333-339
21. Metz JG, Pollard MR, Anderson L, Hayes TR, Lassner MW (2000) Purification of a jojoba embryo fatty acyl-coenzyme A reductase and expression of its cDNA in high erucic acid rapeseed. Plant Physiol 122:635-644
22. Peralta-Yahya PP, Zhang F, del Cardayre SB, Keasling JD (2012) Microbial engineering for the production of advanced biofuels. Nature 488:320-328
23. Reiser S, Somerville C (1997) Isolation of mutants of Acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme A reductase. J Bacteriol 179:2969-2975
24. Rizzo WB, Craft DA (2000) Sjögren-Larsson syndrome: accumulation of free fatty alcohols in cultured fibroblasts and plasma. J Lipid Res 41:1077-1081
25. Rowland O, Zheng H, Hepworth SR, Lam P, Jetter R, Kunst L (2006) CER4 encodes an alcohol-forming fatty acyl-coenzyme A reductase involved in cuticular wax production in Arabidopsis. Plant Physiol 142:866-877
26. Schirmer A, Rude MA, Li X, Popova E, del Cardayre SB (2010) Microbial biosynthesis of alkanes. Science 329:559-562
27. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, del Cardayre SB, Keasling JD (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463:559-562
28. Tan X, Yao L, Gao Q, Wang W, Qi F, Lu X (2011) Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab Eng 13:169-176
29. Teerawanichpan P, Qiu X (2010) Fatty acyl-CoA reductase and wax synthase from Euglena gracilis in the biosynthesis of medium-chain wax esters. Lipids 45:263-273
30. Teerawanichpan P, Robertson AJ, Qiu X (2010) A fatty acyl-CoA reductase highly expressed in the head of honey bee (Apis mellifera) involves biosynthesis of a wide range of aliphatic fatty alcohols. Insect Biochem Mol Biol 40:641-649
31. Vioque J, Kolattukudy PE (1997) Resolution and purification of an aldehyde-generating and an alcohol-generating fatty acyl-CoA reductase from pea leaves (Pisum sativum L.). Arch Biochem Biophys 340:64-72
32. Voelker TA, Davies HM (1994) Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J Bacteriol 176:7320-7327
33. Wahlen BD, Oswald WS, Seefeldt LC, Barney BM (2009) Purification, characterization, and potential bacterial wax production role of an NADPH-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8. Appl Environ Microbiol 75:2758-2764
34. Willis RM, Wahlen BD, Seefeldt LC, Barney BM (2011) Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol. Biochemistry 50:10550-10558
35. Yuan L, Voelker TA, Hawkins DJ (1995) Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc Natl Acad Sci USA 92:10639-10643
36. Zhang F, Ouellet M, Batth TS, Adams PD, Petzold CJ, Mukhopadhyay A, Keasling JD (2012) Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. Metab Eng 14:653-660
37. Zhang X, Li M, Agrawal A, San K-Y (2011) Efficient free fatty acid production in Escherichia coli using plant acyl-ACP thioesterases. Metab Eng 13:713-722
38. Zheng YN, Li LL, Liu Q, Yang JM, Wang XW, Liu W, Xu X, Liu H, Zhao G, Xian M (2012) Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineered Escherichia coli. Microb Cell Fact 11:65