BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Dia, Vermont P ^a   Krishnan, Hari B ^b  

^a UNIVERSITY OF TENNESSEE   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; ANTINEOPLASTIC AGENT; PEPTIDE; PLANT EXTRACT; TRYPSIN INHIBITOR; TUMOR PROTEIN; WATER;

AMINO ACID SEQUENCE; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CHEMISTRY; COLON TUMOR; DRUG EFFECT; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR WEIGHT; MOMORDICA CHARANTIA; PATHOLOGY; TUMOR CELL LINE; TUMOR STEM CELL ASSAY;

AMINO ACID SEQUENCE; ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; COLONIC NEOPLASMS; ETHANOL; HUMANS; MASS SPECTROMETRY; MOLECULAR WEIGHT; MOMORDICA CHARANTIA; NEOPLASM PROTEINS; PEPTIDES; PLANT EXTRACTS; TRYPSIN INHIBITORS; TUMOR STEM CELL ASSAY; WATER;

EID: 84987984841     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep33532     Document Type: Article

References (43)

1. Ferlay, J. et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Intl. J. Cancer. 136, E359-E386 (2015).
2. Hollis, M. et al. MicroRNAs potential utility in colon cancer: Early detection, prognosis, and chemosensitivity. World J. Gastroenterol. 21, 8284-8292 (2015).
3. Siegel, R., DeSantis, C., Jemal A. Colorectal cancer statistics, 2014. CA Cancer J. Clin. 64, 104-117 (2014).
4. Ronducci, L. et al. Aberrant crypt foci in colorectal carcinogenesis, Cell and crypt dynamics. Cell Prolif. 33, 1-18 (2000).
5. Hanahan, D., Weinberg, R. A. Hallmarks of cancer: the next generation. Cell. 144, 646-674 (2011).
6. Sakamaki, K., Satou, Y. Caspases: evolutionary aspects of their functions in vertebrates. J. Fish Biol. 74, 727-753 (2009).
7. Unsain, N., Barker, P. A. New views on the misconstrued: executioner caspases and their diverse non-apoptotic roles. Neuron. 88, 461-474 (2015).
8. Zeng, H., Larazova D. L. Obesity-related colon cancer: Dietary factors and their mechanisms of anticancer action. Clin. Exp. Pharmacol. Physiol. 39, 161-167 (2012).
9. Erdelyn, I. et al. Western-style diets induce oxidative stress and dysregulate immune responses in the colon in a mouse model of sporadic colon cancer. J. Nutr. 139, 2072-2078 (2009).
10. Newmark, H. L. et al. Western-style diet-induced colonic tumors and their modulation by calcium and vitamin D in C57Bl/6 mice: a preclinical model for human sporadic colon cancer. Carcinogenesis. 30, 88-92 (2009).
11. Marshall, J. R. Prevention of colorectal cancer: diet, chemoprevention and lifestyle. Gastroenterol. Clin. North Amer. 37, 73-82 (2008).
12. Song, Y. et al. Dietary fiber and risk of colorectal cancer: a case control study. Asian Pac. J. Cancer Prev. 16, 3747-3752 (2015).
13. Vulcan, A. et al. Fibre intake and incident colorectal cancer depending on fibre source, sex, tumour location and tumour, node, metastasis stage. Brit. J. Nutr. 114, 959-969 (2015).
14. Dandawate, P. R., Subramaniam, D., Padhye, S. B., Anan, S. Bitter melon: a panacea for inflammation and cancer. Chin. J. Nat. Med. 14, 81-100 (2016).
15. Rahman, I. U., Khan, R. U., Rahman K. U., Bashir, M. Lower hypoglycemic but higher antiatherogenic effects of bitter melon than glibenclamide in type 2 diabetic patients. Nutr. J. 14, 13 (2015).
16. Fuanfchan, A. et al. Hypoglycemic effect of bitter melon compared with metformin in newly diagnosed type 2 diabetes patients. J. Ethnopharmacol. 124, 422-428 (2011).
17. Lee-Huang, S. et al. Inhibition of MDA-MB-231 human breast tumor xenografts and HER 2 expression by anti-tumor agents GAP31 and MAP30. Anticancer Res. 20, 653-659 (2000).
18. Kwatra, D. et al. Methanolic extracts of bitter melon inhibit colon cancer stem cells by affecting energy homeostasis and autophagy. Evid. Based Complement. Alternat. Med. 2013, 702869 (2013).
19. Kwatra, D. et al. Bitter melon extracts enhance the activity of chemotherapeutic agents through the modulation of multiple drug resistance. J. Pharm. Sci. 102, 4444-4454 (2013).
20. Zhang, C. Z., Fang, E. F., Zhang, H. T., Liu, L. L., Yun, J. P. Momordica charantia lectin exhibits antitumor activity towards hepatocellular carcinoma. Invest. New Drugs. 33, 1-11 (2015).
21. Miura, S., Funatsu, G. Isolation and amino acid sequences of two trypsin inhibitors from the seeds of bitter gourd (Momordica charantia). Biosci. Biotechnol. Biochem. 59, 469? 473 (1995).
22. Fereidunian, A., Sadeghalvad, M., Oscoie, M. O., Mostafaie, A. Soybean Bowman-Birk protease inhibitor (BBI): identification of the mechanisms of BBI suppressive effect on growth of two adenocarcinoma cell lines: AGS and HT-29. Arch. Med. Res. 45, 455-461 (2014).
23. Fang, E. F., Wong J. H., Ng, T. B. Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans. J. Biosci. Bioeng. 109, 211-217 (2010).
24. Clemente, A., Marin-Manzano, M. C., Jimenez, E., Arques, M. C., Domoney, C. The anti-proliferative effect of TI1B, a major Bowman-Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition. Brit. J. Nutr. 108, 5135-5144 (2012).
25. Clemente, A., Moreno, F. J., Marin-Manzano, M. C., Jimenez, E., Domoney, C. The cytotoxic effect of Bowman-Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases. Mol. Nutr. Food Res. 54, 396-405 (2010).
26. Caccialupi, P. et al. Bowman-Birk inhibitors in lentil: Heterologous expression, functional characterisation and anti-proliferative properties in human colon cancer cells. Food Chem. 120, 1058-1066 (2010).
27. Asiamah, D. et al. Chemopreventive potential of bitter melon ( Momordica charantia) against precancerous lesions in the colon of fisher 344 male rats. Intl. J. Cancer Res. 7, 36-46 (2011).
28. Huang, C. Y., Yu, L. C. Pathophysiological mechanisms of death resistance in colorectal carcinoma. World J. Gastroenterol. 21, 11777-11792 (2015).
29. Renault, T. T., Chipuk, J. E. Death upon a kiss: mitochondrial outer membrane composition and organelle communication govern sensitivity to BAK/BAX-dependent apoptosis. Chem. Biol. 21, 114-123 (2014).
30. Chipuk, J. E. et al. Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proc. Natl. Acad. Sci. USA. 105, 20327-20332 (2008).
31. Mukhopadhyay, S., Panda, O. K., Sinha, N., Das, D. N., Bhutia S. K. Autophagy and apoptosis: where do they meet? Apoptosis. 19, 555-566 (2014).
32. Hamacher-Brady, A., Brady, N. R. Bax/Bak-dependent, Drp1-independent Targeting of X-linked Inhibitor of Apoptosis Protein (XIAP) into Inner Mitochondrial Compartments Counteracts Smac/DIABLO-dependent Effector Caspase Activation. J. Biol. Chem. 290, 22005-22018 (2015).
33. Nehls, O. et al. Studies on p53, Bax and Bcl-2 protein expression and microsatellite instability in stage III (UICC) colon cancer treated by adjuvant chemotherapy: major prognostic impact of proapoptotic Bax. Brit. J. Cancer. 96, 1409-1418 (2007).
34. Nehls, O. et al. Low Bax protein expression correlates with disease recurrence in preoperatively irradiated rectal carcinoma. Intl. J. Radiat. Oncol. Biol. Phys. 61, 85-91 (2005).
35. Khodapasand, E., Jafarzadeh, N., Farrokhi, F., Kamalidehghan, B., Houshmand, M. I. S. Bax/Bcl-2 ratio considered as a prognostic marker with age and tumor location in colorectal cancer? Iran Biomed. J. 19, 69-75 (2015).
36. Konori, M., Ohnishi-Kameyama, M., Akimoto, Y., Yukizaki, C., Yoshida, M. Alpha-eleosteraic acid and its dihydroxyderivative are major apoptosis-inducing components of bitter gord. J. Agric. Food Chem. 56, 10515-10520 (2008).
37. Yasui, Y. et al. Bitter gourd seed fatty acid rich in 9c,11t, 13t-conjugated linolenic acid induces apoptosis and up-regulateds the GADD45, p53 and PPARgamma in human colon cancer Caco-2 cells. Prostaglandin Leukot. Essent. Fatty Acids. 73, 113-119 (2005).
38. Lim, T. G. et al. Curcumin suppresses proliferation of colon cancer cells by targeting CDK2. Cancer Prev. Res. (Phila). 7, 466-474 (2014).
39. Abbas, T., Dutta, A. p21 in cancer: intricate networks and multiple activities. Nat. Rev. Cancer. 9, 400-414 (2009).
40. Palavalli, M. H., Natarajan, S., Wang, T., Krishnan, H. B. Imbibition of soybean seeds in warm water results in the release of copious amounts of Bowman-Birk protease inhibitor, a putative anticarcinogenic agent. J. Agric. Food Chem. 60, 3135-3143 (2012).
41. Krishnan, H. B., Oehrle, N. W., Natarajan, S. S. A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: a case study using Glycine max. Proteomics. 9, 3174-3188 (2009).
42. Klomklao, S., Benjakul, S., Kishimura, H., Osako, K., Tanaka, M. A heat-stable trypsin inhibitor in adzuki bean (Vigna angularis): effect of extraction media, purification and biochemical characteristics. Intl. J. Food Sci. Tech. 45, 163-169 (2010).
43. Chou, T. C. Drug combination studies and their synergy quantification using the Chou-Talalay method. Cancer Res. 70, 440-446 (2010).