The anti-proliferative effect of TI1B, a major Bowman-Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition

British Journal of Nutrition

Volumn 108, Issue SUPPL. 1, 2012, Pages

  Clemente, Alfonso ^a   Carmen Marin Manzano M ^b   Jiménez, Elisabeth ^a   Carmen Arques, M ^a   Domoney, Claire ^a  

^a CSIC   (Spain)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

Bowman Birk inhibitors; Cell proliferation; Colorectal cancer cells; Pea; Protease inhibition; Serine proteases

Indexed keywords

BOWMAN BIRK INHIBITOR; CHYMOTRYPSIN; MUTANT PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; SERINE PROTEINASE; TI 1B; TRYPSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIPROLIFERATIVE ACTIVITY; ARTICLE; CANCER CELL; CANCER GROWTH; CANCER INHIBITION; CANCER PREVENTION; CANCER STAGING; CELL PROLIFERATION; COLON CANCER; COLON CARCINOGENESIS; CONCENTRATION RESPONSE; ENZYME INHIBITION; FIBROBLAST; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; PEA; PICHIA PASTORIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN TARGETING; SITE DIRECTED MUTAGENESIS;

ANTICARCINOGENIC AGENTS; CELL PROLIFERATION; CHYMOTRYPSIN; CLONING, MOLECULAR; COLORECTAL NEOPLASMS; GENE EXPRESSION; HT29 CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED; PEAS; PICHIA; PLANT PROTEINS; RECOMBINANT PROTEINS; SEEDS; TRYPSIN; TRYPSIN INHIBITORS;

EID: 84865426338     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S000711451200075X     Document Type: Article

References (33)

1. Jemal A, Siegel R, Ward E, et al. (2009) Cancer statistics, 2009. Cancer J Clin 59, 225-249.
2. Cummings JH & Bingham SA (1998) Fortnightly review-diet and the prevention of cancer. Br Med J 317, 1636-1640.
3. Reddy BS (2000) Novel approaches to the prevention of colon cancer by nutritional manipulation and chemoprevention. Cancer Epidemiol Biomark Prev 9, 239-247. (Pubitemid 30165435)
4. MacFarlane AJ & Stover PJ (2007) Factors in gastrointestinal cancers: convergence of genetic, nutritional and inflammatory. Nutr Rev 65, S157-S166.
5. Kennedy AR, Billings PC, Wan XS, et al. (2002) Effects of Bowman-Birk inhibitor on rat colon carcinogenesis. Nutr Cancer 43, 174-186. (Pubitemid 36134092)
6. Clemente A & Domoney C (2006) Biological significance of polymorphism in plant protease inhibitors from the Bowman-Birk class. Curr Prot Pept Sci 7, 201-216. (Pubitemid 43881003)
7. Clemente A & Domoney C (2007) Therapeutic properties of legume protease inhibitors from the Bowman-Birk class. In Recent Progress in Medicinal Plants, pp. 397-417 [JN Govil, VK Singh and RK Sharma, editors]. vol. 20, Houston, TX: Studium Press.
8. Clemente A, Sonnante G & Domoney C (2011) Bowman-Birk inhibitors from legumes and human gastrointestinal health: current status and perspectives. Curr Prot Pept Sci 12, 358-373.
9. Chen P, Rose J, Love R, et al. (1992) Reactive sites of an anticarcinogenic Bowman-Birk proteinase inhibitor are similar to other trypsin inhibitors. J Biol Chem 267, 1990-1994.
10. Ramasarma PR, Appu Rao AG & Rao DR (1995) Role of disulfide linkages in structure and activity of proteinase inhibitor from horsegram (Dolichos biflorus). Biochim Biophys Acta 1248, 35-42.
11. Trivedi MV, Laurence JS & Siahann TJ (2009) The role of thiols and disulfides on protein stability. Curr Prot Pept Sci 10, 614-625.
12. Clemente A, Jimenez E, Maŕn-Manzano MC, et al. (2008) Active Bowman-Birk inhibitors survive gastrointestinal digestion at the terminal ileum of pigs fed chickpea-based diets. J Sci Food Agric 88, 523-531.
13. Maŕn-Manzano MC, Ruiz R, Jimenez E, et al. (2009) Anticarcinogenic soyabean Bowman-Birk inhibitors survive faecal fermentation in their active form and do not affect the microbiota composition in vitro. Br J Nutr 101, 967-971.
14. Kennedy AR, Szuhaj BF, Newberne PM, et al. (1993) Preparation and production of a cancer chemopreventive agent, Bowman-Birk inhibitor concentrate. Nutr Cancer 19, 281-302. (Pubitemid 23204845)
15. Clemente A, Moreno J, Maŕn-Manzano MC, et al. (2010) The cytotoxic effect of Bowman-Birk isoinhibitors from soybean on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases. Mol Nutr Food Res 54, 396-405.
16. Clemente A, Gee JM, Johnson IT, et al. (2005) Pea (Pisum sativum L.) protease inhibitors from the Bowman-Birk class influence the growth of human colorectal adenocarcinoma HT29 cells in vitro. J Agric Food Chem 53, 8979-8986. (Pubitemid 41735661)
17. Caccialupi P, Ceci LR, Siciliano RA, et al. (2010) Bowman-Birk inhibitors in lentil: heterologous expression, functional characterisation and anti-proliferative properties in human colon cancer cells. Food Chem 120, 1058-1066.
18. Domoney C. (1999) Inhibitors of legume seeds. In Seed Proteins, pp. 635-655 [PR Shewry and R Casey, editors]. The Netherlands: Kluwer Academic Publishers.
19. Domoney C, Welham T, Ellis N, et al. (2002) Three classes of proteinase inhibitor gene have distinct but overlapping patterns of expression in Pisum sativum plants. Plant Mol Biol 48, 319-329. (Pubitemid 34128533)
20. Ho S, Hunt H, Horton R, et al. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59. (Pubitemid 19125653)
21. Domoney C & Welham T (1992) Trypsin inhibitors in Pisum: variation in amount and pattern of accumulation in developing seed. Seed Sci Res 2, 147-154.
22. Clemente A, MacKenzie DA, Jeenes DJ, et al. (2004) The effect of variation within inhibitory domains on the activity of pea protease inhibitors from the Bowman-Birk class. Protein Express Purif 36, 106-114. (Pubitemid 38836771)
23. Copeland RA, Lombardo D, Giannaras J, et al. (1995) Estimating Ki values for tight-binding inhibitors from doseresponse plots. Bioorg Med Chem Lett 5, 1947-1952.
24. Domoney C, Welham T, Sidebottom C, et al. (1995) Multiple isoforms of Pisum trypsin inhibitors result from modification of two primary gene products. FEBS Lett 360, 15-20.
25. Schechter I&BergerA(1967)Onthe size of the active site inproteases. I. Papain. Biochem Biophys Res Commun 27, 157-162.
26. Damaso MC, Almeida MS, Kurtenbach E, et al. (2003) Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris. Appl Environ Microbiol 69, 6064-6072. (Pubitemid 37287580)
27. Chen Z,Wang D, Cong Y, et al. (2011) Recombinant antimicrobial peptide hPAB-b expressed in Pichia pastoris, a potential agent active against methicillin-resistant Staphylococcus aureus. Appl Microbiol Biotechnol 89, 281-291.
28. Scarafoni A, Consonni A, Galbusera V, et al. (2008) Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds. Phytochem 69, 1820-1825.
29. Kennedy AR (1998) Chemopreventive agents: protease inhibitors. Pharmacol Ther 78, 167-209. (Pubitemid 28255148)
30. Kennedy AR (1998) The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent. Am J Clin Nutr 68, 1406s-1412s. (Pubitemid 28550921)
31. Singh RR & Appu Rao AG (2002) Reductive unfolding and oxidative refolding of a Bowman-Birk inhibitor from horsegram seeds (Dolichos biflorus): evidence for "hyperreactive" disulfide bonds and rate-limiting nature of disulfide isomerisation in folding. Biochim Biophys Acta 1597, 280-291. (Pubitemid 34607258)
32. Volpicella M, Ceci LR, Cordewener J, et al. (2003) Properties of purified gut trypsin from Helicoverpa zea, adapted to protease inhibitors. Eur J Biochem 270, 10-19. (Pubitemid 36056933)
33. Yavelow J, Collins M, Birk Y, et al. (1985) Nanomolar concentrations of Bowman-Birk soybean protease inhibitor suppress X-ray induced transformation in vitro. Proc Natl Acad Sci USA 82, 5395-5399.