Generation and evaluation of Myostatin knock-out rabbits and goats using CRISPR/Cas9 system

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Guo, Rihong ^a   Wan, Yongjie ^a   Xu, Dan ^b   Cui, Libin ^c   Deng, Mingtian ^a   Zhang, Guomin ^a   Jia, Ruoxin ^a   Zhou, Wenjun ^a   Wang, Zhen ^a   Deng, Kaiping ^a   Huang, Mingrui ^a   Wang, Feng ^a   Zhang, Yanli ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Akeagen   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MYOSTATIN;

ANIMAL; CRISPR CAS SYSTEM; GENE KNOCKOUT; GENETICS; GOAT; GROWTH, DEVELOPMENT AND AGING; LEPORIDAE; METABOLISM; PHENOTYPE; SKELETAL MUSCLE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; GOATS; MUSCLE, SKELETAL; MYOSTATIN; PHENOTYPE; RABBITS;

EID: 84978716213     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep29855     Document Type: Article

References (28)

1. McPherron, A. C., Lawler, A. M., Lee, S. J. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387, 83-90 (1997).
2. Catipovic, B. Myostatin mutation associated with gross muscle hypertrophy in a child. New Engl. J. Med. 351, 1030-1030 (2004).
3. Hadjipavlou, G., Matika, O., Clop, A., Bishop, S. C. Two single nucleotide polymorphisms in the myostatin (GDF8) gene have significant association with muscle depth of commercial Charollais sheep. Anim. Genet. 39, 346-353 (2008).
4. Clop, A. et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat. Genet. 38, 813-818 (2006).
5. Grobet, L. et al. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat. Genet. 17, 71-74 (1997).
6. Proudfoot, C. et al. Genome edited sheep and cattle. Transgenic Res. 24, 147-153 (2005).
7. Cyranoski, D. Super-muscly pigs created by small genetic tweak. Nature 523, 13-14 (2015).
8. Wang, K. et al. Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System. Sci Rep 5, 16623 (2015).
9. Zhang, C. et al. Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance. Mol. Biol. Rep. 39, 3081-3087 (2012).
10. Sternstein, I., Reissmann, M., Maj, D., Bieniek, J., Brockmann, G. A. A new single nucleotide polymorphism in the rabbit (Oryctolagus cuniculus) myostatin (MSTN) gene is associated with carcass composition traits. Anim. Genet. 45, 596-599 (2014).
11. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821 (2012).
12. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
13. Yang, D. S. et al. Effective gene targeting in rabbits using RNA-guided Cas9 nucleases. J. Mol. Cell Biol. 6, 97-99 (2014).
14. Niu, Y. et al. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 156, 836-843 (2014).
15. Whitworth, K. M. et al. Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. Biol. Reprod. 91, 78 (2014).
16. Wang, Y. et al. Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA. Sci. Rep. 5, 8256 (2015).
17. Crispo, M. et al. Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PloS One 10, e0136690 (2015).
18. Ni, W. et al. Efficient gene knockout in goats using CRISPR/Cas9 system. PloS One 9, e106718 (2015).
19. Wang, X. et al. Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system. Sci. Rep. 5, 13878 (2015).
20. Zhou, Z. R. et al. Production of myostatin-targeted goat by nuclear transfer from cultured adult somatic cells. Theriogenology 79, 225-233 (2013).
21. Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281-2308 (2013).
22. Arthur, P. F., Makarechian, M., Price, M. A. Incidence of Dystocia and Perinatal Calf Mortality Resulting from Reciprocal Crossing of Double-muscled and Normal Cattle. Can. Vet. J. 29, 163-167 (1988).
23. Li,W., Teng, F., Li, T., Zhou, Q. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat. Biotechnol. 31, 684-686 (2013).
24. Li, D. et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat. Biotechnol. 31, 681-683 (2013).
25. Chen, Y. et al. Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys. Cell Res. 25, 262-265 (2015).
26. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).
27. Bae, S., Park, J., Kim, J. S. Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNAguided endonucleases. Bioinformatics 30, 1473-1475 (2014).
28. Dong, Y. et al. Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus). Nat. Biotechnol. 31, 135-141 (2013).