Polymerase chain reaction detection of Aspergillus carbonarius in grapes at different time after inoculum

Quality Assurance and Safety of Crops and Foods

Volumn 2, Issue 2, 2010, Pages 66-71

  De Rossi, P ^a   Reverberi, M ^b   Ricelli, A ^c   Fabbri, A A ^b   Fanelli, C ^b  

^a ENEA C R CASACCIA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c CNR   (Italy)

Author keywords

Aspergillus carbonarius; Detection; Grapes; Ochratoxin A; PCR

Indexed keywords

ASPERGILLUS; BIOCHEMISTRY; DNA; KETONES;

ASPERGILLUS CARBONARIUS; DETECTION; DIAGNOSTIC METHODS; GRAPE; HIGH SENSITIVITY; HIGH SPECIFICITY; INOCULA; INTERNAL TRANSCRIBED SPACERS; OCHRATOXIN A; SPECIES-SPECIFIC PRIMERS;

POLYMERASE CHAIN REACTION;

EID: 84977742885     PISSN: 17578361     EISSN: 1757837X     Source Type: Journal    
DOI: 10.1111/j.1757-837X.2010.00057.x     Document Type: Article

References (25)

1. Abarca M.L., Accensi F., Bragulat M.R., Castella G., Cabanes F.J. (2003) Aspergillus carbonarius as the main source of ochratoxin A contamination in dried vine fruits from the Spanish market. Journal of Food Protection, 66, 504-506
2. Accensi F., Abarca M.L., Cano J., Figuera L., Cabanes F.J. (2001) Distribution of ochratoxin A-producing strains in the Aspergillus niger aggregate. Antonie van Leeuwenhoek, 79, 365-370
3. Atoui A., Phong Dao H., Mathieu F., Lebrihi A. (2006) Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. Molecular Nutrition and Food Research, 50, 488-493
4. Atoui A., Mathieu F., Lebrihi A. (2007) Targeting a polyketide synthase gene for Aspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR. International Journal of Food Microbiology, 115, 313-318
5. Battilani P., Pietri A. (2002) Ochratoxin A in grapes and wine. European Journal of Plant Pathology, 108, 639-643
6. Bluhm B.H., Flaherty J.E., Cousin M.A., Woloshuk C.P. (2002) Multiplex polymerase chain reaction assay for the differential detection of trichothecene-and fumonisin-producing species of Fusarium in cornmeal. Journal of Food Protection, 65, 1955-1961
7. Cabañes F.J., Accensi F., Bragulat M.R., Abarca M.L., Castellà G., Minguez S., Pons A. (2002)What is the source of ochratoxin A in wine? International Journal of Food Microbiology, 79, 213-215
8. Dao H.P., Mathieu F., Lebrihi A. (2005) Two primer pairs to detect OTA producers by PCR method. International Journal of Food Microbiology, 104, 61-67
9. Esteban A., Abarca M.L., Bragulat M.R., Cabañes F.J. (2004) Effects of temperature and incubation time on production of ochratoxin A by black aspergilli. Microbiology, 155, 861-866
10. Festas I., Herbert P., Santos L., Cabral M., Barros P., Alves A. (2000) Ochratoxin A in some Portuguese wines: method validation and screening in PortWine and Vinho Verde. American Journal of Enology and Viticulture, 51, 150-154
11. Heenan C.N., Shaw K.J., Pitt J.I. (1998) Ochratoxin A production by Aspergillus carbonarius and A. niger isolates and detection using coconut cream agar. Journal of Food Mycology, 1, 67-72
12. Henry T., Iwen P.C., Hinrichs S.H. (2000) Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. Journal of Clinical Microbiology, 38, 510-1515
13. Horie Y. (1995) Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus section Nigri. Nippon Kingakukai Kaiho, 36, 73-76
14. Markaki P., Delpont Binet C., Grosso F., Dragacci S. (2001) Determination of ochratoxin A in red wine and vinegar by immunoaffinity high-pressure liquid chromatography. Journal of Food Protection, 64, 533-537
15. Mulè G., Susca A., Logrieco A., Stea G., Visconti A. (2006) Development of a quantitative real time PCR assay for the detection of Aspergillus carbonarius in grapes. International Journal of Food Microbiology, 111, 28-34
16. Patino B., Gonzàlez-Salgado A., Gonzàlez-Jáen M.T., Vàzquez C. 2005 PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species. International Journal Food Microbiology, 104, 207-214
17. Pietri A., Bertuzzi T., Pallaroni L., Piva G. (2001) Occurrence of ochratoxin A in Italian wines. Food Additives and Contaminants, 18, 647-654
18. Selma MV., Martinez-Culebras P.V., Aznar R. (2008) Real Time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes. International Journal of Food Microbiology, 122, 126-134
19. Serra R., Abrunhosa L., Kozakiewicz Z., Venancio A. (2003) Black Aspergillus species as ochratoxin A producers in Portuguese wine grapes. International Journal of Food Microbiology, 88, 63-68
20. Soleas G.J., Yan J., Goldberg D.M. (2001) Assay of ochratoxin A in wine and beer by high-pressure liquid chromatography photodiode array and gas chromatography mass selective detection. Journal of Agricultural and Food Chemistry, 49, 2733-2740
21. Téren J., Varga J., Hamari Z., Rinyu E., Kevei F. (1996) Immunochemical detection of ochratoxin A in black Aspergillus strains. Mycopathologia, 134, 171-176
22. Varga J., Kozakiewicz Z. (2006) Ochratoxin A in grapes and grape derived products. Trends in Food Science and Technology, 17, 72-81
23. Visconti A., Pascale M., Centonze G. (2000) Determination of ochratoxin A in wine bymeans of immunoaffinity column cleanup and HPLC. Journal of Chromatography A, 864, 89-101
24. Zhao J., Kong F., Li R., Wang X., Wan Z., Wang D. (2001) Identification of Aspergillus fumigatus and related species by nested PCR targeting ribosomal DNA internal transcribed spacer regions. Journal of Clinical Microbiology, 39, 2261-2266
25. Zimmerli B., Dick R. (1996) Ochratoxin A in table wine and grape-juice: occurrence and risk assessment. Food Additives and Contaminants, 13, 655-668