Optimization of a cytochrome P450 oxidation system for enhancing protopanaxadiol production in Saccharomyces cerevisiae

Biotechnology and Bioengineering

Volumn 113, Issue 8, 2016, Pages 1787-1795

  Zhao, Fanglong ^a   Bai, Peng ^a   Liu, Ting ^a   Li, Dashuai ^a   Zhang, Xiangmei ^a   Lu, Wenyu ^a,b   Yuan, Yingjin ^a,b  

^a TIANJIN UNIVERSITY   (China)

^b SynBio Research Platform   (China)

Author keywords

dammarenediol II; protopanaxadiol; protopanaxadiol synthase; Saccharomyces cerevisiae; self sufficient P450s; synthetic biology

Indexed keywords

ACTIVATION ANALYSIS; CATALYST ACTIVITY; FERMENTATION; PLANTS (BOTANY); PROTEINS;

DAMMARENEDIOL-II; PROTOPANAXADIOL; SELF-SUFFICIENT P450S; SYNTHASES; SYNTHETIC BIOLOGY;

YEAST;

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; CYTOCHROME P450; GERANYLTRANSFERASE; HYBRID PROTEIN; PANAX GINSENG CYTOCHROME P450 TYPE PROTOPANAXADIOL SYNTHASE; PPDS ATR1 FUSION PROTEIN; PROTOPANAXADIOL; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; SQUALENE MONOOXYGENASE; SQUALENE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

ARABIDOPSIS THALIANA; ARTICLE; BIOREACTOR; BIOTECHNOLOGICAL PRODUCTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MECHANISM; FED BATCH FERMENTATION; FUNGAL STRAIN; HETEROLOGOUS EXPRESSION; IN VIVO STUDY; NONHUMAN; OXIDATION; PROCESS OPTIMIZATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

EID: 84977124867     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25934     Document Type: Article

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