Laboratory evolution of P450 BM3 for mediated electron transfer yielding an activity-improved and reductase-independent variant

Protein Engineering, Design and Selection

Volumn 21, Issue 1, 2008, Pages 29-35

  Nazor, Jovana ^a   Dannenmann, Stephanie ^a   Adjei, Richard Obeng ^a   Fordjour, Yaw Boafo ^a   Ghampson, Isaac Tyrone ^a   Blanusa, Milan ^a   Roccatano, Danilo ^a   Schwaneberg, Ulrich ^a  

^a JACOBS UNIVERSITY BREMEN   (Germany)

Author keywords

Cofactor; Directed evolution; High throughput screening; Mediator; Monooxygenase

Indexed keywords

COST EFFECTIVENESS; ENZYMES; REACTION KINETICS; SYNTHESIS (CHEMICAL);

DIRECTED EVOLUTION; HIGH-THROUGHPUT SCREENING; MONOOXYGENASE;

ELECTRON TRANSITIONS;

CYTOCHROME P450; OXIDOREDUCTASE;

ARTICLE; BACILLUS MEGATERIUM; CATALYSIS; ELECTRON TRANSPORT; ENZYME ACTIVITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

BACTERIAL PROTEINS; COBALT; COENZYMES; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; ELECTRON TRANSPORT; KINETICS; LAURATES; MIXED FUNCTION OXYGENASES; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; NADP; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; ZINC;

BACILLUS MEGATERIUM;

EID: 38349110286     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm074     Document Type: Article

References (46)

1. Adlercreutz,P. (1996) Biocatal. Biotransformation, 14, 1-30.
2. Appel,D., Lutz-Wahl,S., Fischer,P., Schwaneberg,U. and Schmid,R.D. (2001) J. Biotechnol., 88, 167-171.
3. Barnes,H.J., Arlotto,M.P. and Waterman,M.R. (1991) Proc. Natl Acad. Sci. USA, 88, 5597-5601.
4. Belev,T.N., Singh,M. and McCarthy,J.E.G. (1991) Plasmid, 26, 147-150.
5. Bistolas,N., Christenson,A., Ruzgas,T., Jung,C., Scheller,F.W. and Wollenberger,U. (2004) Biochem. Biophys. Res. Commun., 314, 810-816.
6. Cirino,P.C. and Arnold,F.H. (2003) Angew. Chem. Int. Edit., 42, 3299-3301.
7. Daff,S.N., Chapman,S.K., Turner,K.L., Holt,R.A., Govindaraj,S., Poulos,T.L. and Munro,A.W. (1997) Biochemistry, 36, 13816-13823.
8. Drauz,K., Eils,S. and Schwarm,M. (2002) Chimica Oggi, 20, 15-21.
9. Estabrook,R.W., Shet,M.S., Faulkner,K. and Fisher,C.W. (1996) Endocr. Res., 22, 665-671.
10. Fantuzzi,A., Fairhead,M. and Gilardi,G. (2004) J. Am. Chem. Soc., 126, 5040-5041.
11. Gilardi,G., Meharenna,Y.T., Tsotsou,G.E., Sadeghi,S.J., Fairhead,M. and Giannini,S. (2002) Biosens. Bioelectron., 17, 133-145.
12. Hollmann,F., Witholt,B. and Schmid,A. (2002) J. Mol. Catal. B Enzym., 19, 167-176.
13. Joo,H., Arisawa,A., Lin,Z.L. and Arnold,F.H. (1999a) Chemis. Biol., 6, 699-706.
14. Joo,H., Lin,Z.L. and Arnold,F.H. (1999b) Nature, 399, 670-673.
15. Li,H.Y. and Poulos,T.L. (1997) Nat. Struct. Biol., 4, 140-146.
16. Lvov,Y.M., Lu,Z.Q., Schenkman,J.B. and Rusling,J.F. (1998) Abstr. Am. Chem. Soc., 215, U486-U486.
17. Matsumura,H., Wiwatchaiwong,S., Nakamura,N., Yohda,M. and Ohno,H. (2006) Electrochem. Commun., 8, 1245-1249.
18. Mayhew,M.P., Reipa,V., Holden,M.J. and Vilker,V.L. (2000) Biotechnol. Progress, 16, 610-616.
19. Munge,B., Estavillo,C., Schenkman,J.B. and Rusling,J.F. (2003) Chembiochem, 4, 82-89.
20. Murataliev,M.B., Klein,M., Fulco,A. and Feyereisen,R. (1997) Biochemistry, 36, 8401-8412.
21. Narhi,L.O. and Fulco,A.J. (1987) J. Biol. Chem., 262, 6683-6690.
22. Nazor,J. and Schwaneberg,U. (2006) Chembiochem, 7, 638-644.
23. Omura,T. and Sato,R. (1964) J. Biol. Chem., 239, 2379-2385.
24. Ravichandran,K.G., Boddupalli,S.S., Hasemann,C.A., Peterson,J.A. and Deisenhofer,J. (1993) Science, 261, 731-736.
25. Reipa,V., Mayhew,M.P., Holden,M.J. and Vilker,V.L. (2002) Chem. Commun., 318-319.
26. Reipa,V., Mayhew,M.P. and Vilker,V.L. (1997) Proc. Natl Acad. Sci. USA, 94, 13554-13558.
27. Ruettinger,R.T., Wen,L.P. and Fulco,A.J. (1989) J. Biol. Chem., 264, 10987-10995.
28. Schwaneberg,U., Appel,D., Schmitt,J. and Schmid,R.D. (2000) J. Biotechnol., 84, 249-257.
29. Schwaneberg,U., Otey,C., Cirino,P.C., Farinas,E. and Arnold,F.H. (2001) J. Biomol. Screening, 6, 111-117.
30. Schwaneberg,U., Schmidt-Dannert,C., Schmitt,J. and Schmid,R.D. (1999a) Anal. Biochem., 269, 359-366.
31. Schwaneberg,U., Sprauer,A., Schmidt-Dannert,C. and Schmid,R.D. (1999b) J. Chromatogr. A, 848, 149-159.
32. Sevrioukova,I.F., Li,H.Y., Zhang,H., Peterson,J.A. and Poulos,T.L. (1999) Proc. Natl Acad. Sci. USA, 96, 1863-1868.
33. Shukla,A., Gillam,E.M., Mitchell,D.J. and Bernhardt,P.V. (2005) Electrochem. Commun., 7, 437-442.
34. Shumyantseva,V.V., Bulko,T.V., Rudakov,Y.O., Kuznetsova,G.P., Samenkova,N.F., Lisitsa,A.V., Karuzina,I.T. and Archakov,A.I. (2007) J. Inorg. Biochem., 101, 859-865.
35. Shumyantseva,V.V., Bulko,T.V., Schmid,R.D. and Archakov,A.I. (2000) Biofizika, 45, 1013-1018.
36. Shumyantseva,V.V., Ivanov,Y.D., Bistolas,N., Scheller,F.W., Archakov,A.I. and Wollenberger,U. (2004) Anal. Chem., 76, 6046-6052.
37. Stampfer,W., Kosjek,B., Moitzi,C., Kroutil,W. and Faber,K. (2002) Angew. Chem. Int. Ed., 41, 1014-1017.
38. Udit,A.K., Hill,M.G. and Gray,H.B. (2006) Langmuir, 22, 10854-10857.
39. Donk van der,W.A. and Zhao,H.M. (2003) Curr. Opin. Biotechnol., 14, 421-426.
40. Wolberg,M., Hummel,W., Wandrey,C. and Muller,M. (2000) Angew. Chem. Int. Ed., 39, 4306-4308.
41. Wong,T.S., Arnold,F.H. and Schwaneberg,U. (2004) Biotechnol. Bioeng., 85, 351-358.
42. Wong,T.S. and Schwaneberg,U. (2003) Curr. Opin. Biotechnol., 14, 590-596.
43. Wong,T.S., Wu,N., Roccatano,D., Zacharias,M. and Schwaneberg,U. (2005) J. Biomol. Screening, 10, 246-252.
44. Zheng,L., Baumann,U. and Reymond,J.L. (2004) Nucleic Acids Res., 32.
45. Zhu,Z.W., Momeu,C., Zakhartsev,M. and Schwaneberg,U. (2006) Biosens. Bioelectron., 21, 2046-2051.
46. Zimmer,T., Kaminski,K., Scheller,U., Vogel,F. and Schunck,W.H. (1995) DNA Cell Biol., 14, 619-628.