The isolation and characterization of dammarenediol synthase gene from Panax quinquefolius and its heterologous co-expression with cytochrome P450 gene PqD12H in yeast

Functional and Integrative Genomics

Volumn 14, Issue 3, 2014, Pages 545-557

  Wang, Le ^a   Zhao, Shou Jing ^a   Cao, Hao Jie ^a   Sun, Yao ^b  

^a JILIN UNIVERSITY   (China)

^b CHANGCHUN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Dammarenediol synthase; Heterologous co expression; Overexpression; Panax quinquefolius; Protopanaxadiol

Indexed keywords

GINSENOSIDE; PROTOPANAXADIOL; CYTOCHROME P450; DAMMARENEDIOL; SAPOGENIN; SAPONIN; TRITERPENE; VEGETABLE PROTEIN;

ARTICLE; CONTROLLED STUDY; D12H GENE; DAMMARENEDIOL SYNTHASE GENE; GENE; GENE EXPRESSION; GENE IDENTIFICATION; GENE ISOLATION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PANAX QUINQUEFOLIUS; PLANT GENE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; AMINO ACID SEQUENCE; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; PANAX; PHYLOGENY; PLANT ROOT; SACCHAROMYCES CEREVISIAE;

PANAX; PANAX GINSENG; PANAX QUINQUEFOLIUS;

AMINO ACID SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PANAX; PHYLOGENY; PLANT PROTEINS; PLANT ROOTS; SACCHAROMYCES CEREVISIAE; SAPOGENINS; SAPONINS; TRITERPENES;

EID: 84906337960     PISSN: 1438793X     EISSN: 14387948     Source Type: Journal    
DOI: 10.1007/s10142-014-0384-1     Document Type: Article

References (29)

1. Akihisa T, Tokuda H, Ukiya M, Suzuki T, Enjo F, Koike K et al (2004) 3-Epicabraleahydroxylactone and other triterpenoids from Camellia oil and their inhibitory effects on Epstein-Barr virus activation. Chem Pharm Bull 52:153-156 (Pubitemid 41685570)
2. Assinewe VA, Baum BR, Gagnon D, Arnason JT (2003) Phytochemistry of wild populations of Panax quinquefolius L. (North American ginseng). J Agric Food Chem 51:4549-4553
3. Attele AS, Wu JA, Yuan CS (1999) Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 58:1685-1693 (Pubitemid 29505408)
4. Banno N, Akihisa T, Tokuda H, Yasukawa K, Higashihara H, Ukiya M et al (2004) Triterpene acids fromthe leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects. Biosci Biotechnol Biochem 68:85-90 (Pubitemid 39251568)
5. Cai J, Yue XZ, Zhong JJ (2007) Protopanaxadiol 6-hydroxylase and its role in regulating the Ginsenoside heterogeneity in Panax notoginseng cells. Biotechnol Bioeng 100:933-939
6. Coleman CI, Hebert JH, Reddy P (2003) The effects of Panax ginseng on quality of life. J Clin Pharm Ther 28:5-15 (Pubitemid 36313170)
7. Dey L, Xie JT, Wang A, Wu J, Maleckar SA, Yuan CS (2003) Anti-hyperglycemic effects of ginseng: comparison between root and berry. Phytomedicine 10:600-605 (Pubitemid 37092688)
8. Gietz D, St Jean A, Woods RA, Schiestl RH (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425
9. Han JY, Kwon YS, Yang DC, Jung YR, Choi YE (2006) Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng. Plant Cell Physiol 47:1653-1662 (Pubitemid 44968260)
10. Han JY, Kim HJ, Kwon YS, Choi YE (2011) The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng. Plant Cell Physiol 52:2062-2073
11. Hey SJ, Powers SJ, Beale MH, Hawkins ND, Ward JL, Halford NG (2006) Enhanced seed phytosterol accumulation through expression of a modified HMG-CoA reductase. Plant Biotechnol J 4:219-229
12. Kiefer D, Pantuso T (2003) Panax ginseng. Am Fam Physician 68:1539-1542 (Pubitemid 37296048)
13. Kim OT, Bang KW, Kim YC, Hyun DY, Kim MY, Cha SW (2009) Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tissue Organ Cult 98:25-33
14. Kima SJ, Murthy HN, Hahn EJ, Lee HL, Paek KY (2007) Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng (Panax ginseng C.A.Meyer). Sep Purif Technol 56:401-406 (Pubitemid 47176490)
15. Kushiro T, Ohno Y, Shibuya Y, Ebizuka Y (1997) In vitro conversion of 2,3-oxidosqualene into dammarenediol by Panax ginseng microsomes. Biol Pharm Bull 20:292-294 (Pubitemid 27148983)
16. Kushiro T, Shibuya M, Ebizuka Y (1998) b-Amyrin synthase: cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants. Eur J Biochem 256:238-244 (Pubitemid 28399118)
17. Landl KM, Klösch B, Turnowsky F (1996) ERG1, encoding squalene epoxidase, is located on the right arm of chromosome VII of Saccharomyces cerevisiae. Yeast 12:609-613 (Pubitemid 26176934)
18. Lee MH, Jeong JH, Seo JW, Shin CG, Kim YS, In JG et al (2004) Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant Cell Physiol 45:976-984
19. Liang Y, Zhao S (2008) Progress in understanding of ginsenoside biosynthesis. Plant Biol (Stuttg) 10:415-421 (Pubitemid 351832386)
20. Poehland BL, Carte BK, Francis TA, Hyland LJ, Allaudeen HS (1987) In vitro antiviral activity of dammar resin triterpenoids. J Nat Prod 50:706-713 (Pubitemid 18068612)
21. Samukawa K, Yamashita H, Matsuda H, Kubo M (1995) Simultaneous analysis of saponins in ginseng radix by high performance liquid chromatography. Chem Pharm Bull 43:137-141
22. Sasaki K, Minowa N, Kuzuhara H, Nishiyama S, Omoto S (1997) Synthesis and hepatoprotective effects of soyasapogenol B derivatives. Bioorg Med Chem Lett 7:85-88 (Pubitemid 27008535)
23. Shibata S (2001) Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds. J Korean Med Sci 16:S28-S37
24. Sun Y, Zhao SJ, Liang YL et al (2013) Regulation and differential expression of protopanaxadiol synthase in Asian and American ginseng ginsenoside biosynthesis by RNA interferences. Plant Growth Regul 71(3):207-217
25. Tansakul P, Shibuya M, Kushiro T, Ebizuka Y (2006) Dammarenediol-II synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng. FEBS Lett 580:5143-5149 (Pubitemid 44415784)
26. Usami Y, Liu YN, Lin AS, Shibano M, Akiyama T, Itokawa H et al (2008) Antitumor agents. 261. 20(S)-protopanaxadiol and 20(S)-protopanaxatriol as antiangiogenic agents and total assignment of (1)H NMR spectra. J Nat Prod 71:478-481 (Pubitemid 351549379)
27. Vogler BK, Pittler MH, Ernst E (1999) The efficacy of ginseng. A systematic review of randomised clinical trials. Eur J Clin Pharmacol 55:567-575 (Pubitemid 29489202)
28. Wang CZ, Wu JA, Mcentee E, Yuan CS (2006) Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography. J Agric Food Chem 54:2261-2266
29. Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211-215