Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Zhang, Jian Ping ^a,b   Li, Xiao Lan ^a,b   Neises, Amanda ^c   Chen, Wanqiu ^c   Hu, Lin Ping ^a,b   Ji, Guang Zhen ^a,b   Yu, Jun Yao ^a,b   Xu, Jing ^a,b   Yuan, Wei Ping ^a,b   Cheng, Tao ^a,b,d,e   Zhang, Xiao Bing ^a,b,c  

^a PEKING UNION MEDICAL COLLEGE   (China)

^b INSTITUTE OF HEMATOLOGY AND BLOOD DISEASES HOSPITAL   (China)

^c LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d Collaborative Innovation Center for Cancer Medicine   (China)

^e Tianjin Key Laboratory of Cellular and Molecular Immunology   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GUIDE RNA; RNA;

CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; GENE KNOCKOUT; GENE TARGETING; GENETICS; HEK293 CELL LINE; HUMAN; INDUCED PLURIPOTENT STEM CELL; LENTIVIRUS; MESENCHYMAL STROMA CELL; METABOLISM; MUTATION; PROCEDURES;

CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; HEK293 CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LENTIVIRUS; MESENCHYMAL STROMAL CELLS; MUTATION; RNA; RNA, GUIDE;

EID: 84976417465     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep28566     Document Type: Article

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