Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Bae, Sang Jeong ^a   Kim, Sujin ^a   Hahn, Ji Sook ^a  

^a Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOIN; ACETOLACTATE DECARBOXYLASE; ACETOLACTATE SYNTHASE; ALCOHOL DEHYDROGENASE; BACTERIAL PROTEIN; BUTANEDIOL DEHYDROGENASE; CARBOXYLYASE; MULTIENZYME COMPLEX; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE;

BACILLUS SUBTILIS; ENZYMOLOGY; FERMENTATION; GENE DELETION; GENETICS; LACTOCOCCUS; METABOLISM; MICROBIOLOGY; PROCEDURES; SACCHAROMYCES CEREVISIAE;

ACETOIN; ACETOLACTATE SYNTHASE; ALCOHOL OXIDOREDUCTASES; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CARBOXY-LYASES; FERMENTATION; GENE DELETION; INDUSTRIAL MICROBIOLOGY; LACTOCOCCUS; MULTIENZYME COMPLEXES; NADH, NADPH OXIDOREDUCTASES; SACCHAROMYCES CEREVISIAE;

EID: 84974601942     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep27667     Document Type: Article

References (29)

1. Xiao, Z. & Lu, J. R. Strategies for enhancing fermentative production of acetoin: A review. Biotechnol. Adv. 32, 492-503 (2014).
2. Xiao, Z., Ma, C., Xu, P. & Lu, J. R. Acetoin catabolism and acetylbutanediol formation by Bacillus pumilus in a chemically defined medium. PLoS One 4, e5627 (2009).
3. Chen, H. et al. Shanghai Apple Flavor & Fragrance Co. Ltd. A preparation method of butadione by gas-phase oxidating 3-hydroxybutanone. European patent EP1826194 A1. 2007 Aug 29.
4. Xiao, Z., Hou, X., Lyu, X., Xi, L. & Zhao, J. Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate. Biotechnol. Biofuels 7, 106 (2014).
5. Wang, X. et al. Efficient bioconversion of 2,3-butanediol into acetoin using Gluconobacter oxydans DSM 2003. Biotechnol. Biofuels 6, 155 (2013).
6. Li, S., Xu, N., Liu, L. & Chen, J. Engineering of carboligase activity reaction in Candida glabrata for acetoin production. Metab. Eng. 22, 32-39 (2014).
7. Bai, F. et al. Engineered Serratia marcescens for efficient (3R)-acetoin and (2R, 3R)-2,3-butanediol production. J. Ind. Microbiol. Biotechnol. 42, 779-786 (2015).
8. Xiao, Z. & Xu, P. Acetoin metabolism in bacteria. Crit. Rev. Microbiol. 33, 127-140 (2007).
9. Zhang, X. et al. Mutation breeding of acetoin high producing Bacillus subtilis blocked in 2,3-butanediol dehydrogenase. World J. Microbiol. Biotechnol. 29, 1783-1789 (2013).
10. Sun, J. A., Zhang, L. Y., Rao, B., Shen, Y. L. & Wei, D. Z. Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase. Bioresour. Technol. 119, 94-98 (2012).
11. Zhang, X. et al. The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis. Metab. Eng. 23, 34-41 (2014).
12. Zhang, L. et al. Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae. Green Chem. 18, 1560-1570 (2016).
13. Cambon, B., Monteil, V., Remize, F., Camarasa, C. & Dequin, S. Effects of GPD1 overexpression in Saccharomyces cerevisiae commercial wine yeast strains lacking ALD6 genes. Appl. Environ. Microbiol. 72, 4688-4694 (2006).
14. Kim, S. & Hahn, J. S. Efficient production of 2,3-butanediol in Saccharomyces cerevisiae by eliminating ethanol and glycerol production and redox rebalancing. Metab. Eng. 31, 94-101 (2015).
15. Kim, S. J., Seo, S. O., Jin, Y. S. & Seo, J. H. Production of 2,3-butanediol by engineered Saccharomyces cerevisiae. Bioresour. Technol. 146, 274-281 (2013).
16. Lian, J., Chao, R. & Zhao, H. Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R, 3R)-butanediol. Metab. Eng. 23, 92-99 (2014).
17. Kim, S., Lee, K., Bae, S. J. & Hahn, J. S. Promoters inducible by aromatic amino acids and g-aminobutyrate (GABA) for metabolic engineering applications in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 99, 2705-2714 (2015).
18. Ansell, R., Granath, K., Hohmann, S., Thevelein, J. M. & Adler, L. The two isoenzymes for yeast NAD+dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J. 16, 2179-2187 (1997).
19. Gonzalez, E. et al. Role of Saccharomyces cerevisiae oxidoreductases Bdh1p and Ara1p in the metabolism of acetoin and 2,3-butanediol. Appl. Environ. Microbiol. 76, 670-679 (2010).
20. Chen, X. L., Li, S. B. & Liu, L. M. Engineering redox balance through cofactor systems. Trends Biotechnol. 32, 337-343 (2014).
21. Forster, J., Famili, I., Fu, P., Palsson, B. O. & Nielsen, J. Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network. Genome Res. 13, 244-253 (2003).
22. Heux, S., Cachon, R. & Dequin, S. Cofactor engineering in Saccharomyces cerevisiae: Expression of a H2O-forming NADH oxidase and impact on redox metabolism. Metab. Eng. 8, 303-314 (2006).
23. Hejazi, A. & Falkiner, F. R. Serratia marcescens. J. Med. Microbiol. 46, 903-912 (1997).
24. Gaston, M. A. Enterobacter: an emerging nosocomial pathogen. J. Hosp. Infect. 11, 197-208 (1988).
25. Dai, J. Y., Cheng, L., He, Q. F. & Xiu, Z. L. High acetoin production by a newly isolated marine Bacillus subtilis strain with low requirement of oxygen supply. Process Biochem. 50, 1730-1734 (2015).
26. Zhang, X. et al. Two-stage pH control strategy based on the pH preference of acetoin reductase regulates acetoin and 2,3-butanediol distribution in Bacillus subtilis. PLoS One 9, e91187 (2014).
27. Luo, Q., Wu, J. & Wu, M. Enhanced acetoin production by Bacillus amyloliquefaciens through improved acetoin tolerance. Process Biochem. 49, 1223-1230 (2014).
28. Zhang, L., Chen, S., Xie, H., Tian, Y. & Hu, K. Efficient acetoin production by optimization of medium components and oxygen supply control using a newly isolated Paenibacillus polymyxa CS107. J. Chem. Technol. Biotechnol. 87, 1551-1557 (2012).
29. Mumberg, D., Muller, R. & Funk, M. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156, 119-122 (1995).