RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures

BMC Genomics

Volumn 17, Issue 1, 2016, Pages

  Pellegrino, Maurizio ^a   Sciambi, Adam ^a   Yates, Jamie L ^a   Mast, Joshua D ^a   Silver, Charles ^a   Eastburn, Dennis J ^a  

^a Mission Bio Inc   (United States)

Author keywords

Cell sorting; Droplets; Gene expression; Heterogeneity; Microfluidics; PCR; Single cell; Transcriptome

Indexed keywords

TRANSCRIPTOME;

ARTICLE; CELL ISOLATION; CELL POPULATION; CELL SELECTION; CELLS; CYTOMETRY; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; MICROFLUIDICS; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION ACTIVATED CELL SORTING; PROSTATE CANCER CELL LINE; RARE CELL; RNA SEQUENCE; SINGLE CELL ANALYSIS; CELL LINE; CLUSTER ANALYSIS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HIGH THROUGHPUT SEQUENCING; MULTIPLEX POLYMERASE CHAIN REACTION; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; WORKFLOW;

CELL LINE; CLUSTER ANALYSIS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SINGLE-CELL ANALYSIS; TRANSCRIPTOME; WORKFLOW;

EID: 84969286769     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/s12864-016-2694-2     Document Type: Article

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