A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals: A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis

Journal of Genetics and Genomics

Volumn 43, Issue 5, 2016, Pages 341-348

  Chenouard, Vanessa ^a,b   Brusselle, Lucas ^a,b   Heslan, Jean Marie ^a   Remy, Séverine ^a,b   Ménoret, Séverine ^a,b   Usal, Claire ^a,b   Ouisse, Laure Hélène ^a,b   NGuyen, Tuan Huy ^a   Anegon, Ignacio ^a,b   Tesson, Laurent ^a,b  

^a NANTES UNIVERSITY HOSPITAL   (France)

^b Recombinant Protein Production Facility Federative Research Structure François Bonamy   (France)

Author keywords

Capillary electrophoresis; Genome editing; Genotyping; Heteroduplex mobility assay

Indexed keywords

GENOMIC DNA; HETERODUPLEX;

ARTICLE; AUTOMATION; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; GENE AMPLIFICATION; GENOME EDITING; GENOTYPE; HETERODUPLEX ANALYSIS; HETERODUPLEX MOBILITY ASSAY; LAB ON A CHIP; MICROFLUIDIC ANALYSIS; MICROFLUIDIC CAPILLARY ELECTROPHORESIS; MOSAICISM; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEIC ACID STRUCTURE, METABOLISM AND FUNCTION; POLYMERASE CHAIN REACTION; RAT; RAT MUTANT; WILD TYPE; WORKFLOW; ANIMAL; COST BENEFIT ANALYSIS; DEVICES; ECONOMICS; GENE EDITING; GENOTYPING TECHNIQUE; MUTATION; TIME FACTOR;

ANIMALS; COST-BENEFIT ANALYSIS; ELECTROPHORESIS, CAPILLARY; GENE EDITING; GENOTYPING TECHNIQUES; LAB-ON-A-CHIP DEVICES; MUTATION; RATS; TIME FACTORS;

EID: 84969144846     PISSN: 16738527     EISSN: 18735533     Source Type: Journal    
DOI: 10.1016/j.jgg.2016.04.005     Document Type: Article

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