Enhanced GAD65 production in plants using the MagnICON transient expression system: Optimization of upstream production and downstream processing

Biotechnology Journal

Volumn 11, Issue 4, 2016, Pages 542-553

  Merlin, Matilde ^a   Gecchele, Elisa ^a   Arcalis, Elsa ^b   Remelli, Sabrina ^a   Brozzetti, Annalisa ^c   Pezzotti, Mario ^a   Avesani, Linda ^a  

^a UNIVERSITY OF VERONA   (Italy)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Molecular farming; Protein engineering; Protein purification; Protein solubility; Type 1 diabetes

Indexed keywords

AMINO ACIDS; CYTOLOGY; PRODUCTION PLATFORMS; PROTEINS; PURIFICATION; SOLUBILITY; VACCINES;

MOLECULAR FARMING; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN SOLUBILITY; TYPE 1 DIABETES;

RECOMBINANT PROTEINS;

GLUTAMATE DECARBOXYLASE 65; PHENOL DERIVATIVE; GLUTAMATE DECARBOXYLASE; RECOMBINANT PROTEIN;

AGROBACTERIUM TUMEFACIENS; AMINO TERMINAL SEQUENCE; ARTICLE; CODON; CONTROLLED STUDY; CYTOSOL; DNA SEQUENCE; DOWNSTREAM PROCESSING; DRUG DISTRIBUTION; DRUG PURIFICATION; DRUG SOLUBILITY; DRUG SYNTHESIS; ENZYME ENGINEERING; ENZYME LOCALIZATION; GENE DELETION; GENE DOSAGE; GENE EXPRESSION SYSTEM; INSULIN DEPENDENT DIABETES MELLITUS; NICOTIANA BENTHAMIANA; NICOTIANA TABACUM; NONHUMAN; PALMITOYLATION; PLANT GROWTH; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; QUALITATIVE ANALYSIS; SUSPENSION; TRANSIENT EXPRESSION SYSTEM; TURBIDITY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; TOBACCO; TRANSGENIC PLANT;

CYTOSOL; GLUTAMATE DECARBOXYLASE; HUMANS; MUTATION; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TOBACCO;

EID: 84963542437     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500187     Document Type: Article

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