Expression in Saccharomyces cerevisiae of antigenically and enzymatically active recombinant glutamic acid decarboxylase

Journal of Biotechnology

Volumn 61, Issue 1, 1998, Pages

  Law, Ruby H P ^a   Rowley, Merrill J ^a   Mackay, Ian R ^a   Corner, Brian ^a,b  

^a MONASH UNIVERSITY   (Australia)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Glutamic acid decarboxylase (GAD); Insulin dependent diabetes mellitus (IDDM); Saccharomyces cerevisiae

Indexed keywords

AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; BIOASSAY; CATALYST ACTIVITY; PRECIPITATION (CHEMICAL); PURIFICATION; YEAST;

GLUTAMIC ACID DECARBOXYLASE;

ENZYMES;

GLUTAMATE DECARBOXYLASE; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ARTICLE; ENZYME MODIFICATION; IMMUNOBLOTTING; IMMUNOREACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIOIMMUNOPRECIPITATION; SACCHAROMYCES CEREVISIAE;

DIABETES MELLITUS, TYPE 1; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GLUTAMATE DECARBOXYLASE; HUMANS; IMMUNOSORBENT TECHNIQUES; PROTEIN HYBRIDIZATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SILVER STAINING;

SACCHAROMYCES CEREVISIAE; SUIDAE;

EID: 0032568191     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00013-3     Document Type: Article

References (28)

1. r autoantibodies as predictors of insulin-dependent diabetes. Lancet. 335:1990;1357-1360.
2. Baekkeskov S., Aanstoot H.J., Christgau S., Reetz A., Solimena M., Cascalho M., Folli F., Richter-Olesen H., DeCamilli P., Camilli P.D. Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature. 347:1990;151-156.
3. r human islet cell antigen precede the clinical onset of insulin-dependent diabetes. J. Clin. Invest. 79:1987;926-934.
4. Blindermann J.M., Maitre M., Ossola L., Mandel P. Purification and some properties of L-glutamate decarboxylase from human brain. Eur. J. Biochem. 86:1978;143-152.
5. Bu D.F., Tobin A.J. The exon-intro organization of genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derived from a common ancestral GAD. Genomics. 21:1994;222-228.
6. Bu D.F., Erlander M.G., Hitz B.C., Tillakaratne N.J., Kaufman D.L., Wagner-McPherson C.B., Evans G.A., Tobin A.J. Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Proc. Natl. Acad. Sci. U.S.A. 89:1992;2115-2119.
7. Chang Y.C., Gottlieb D.I. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J. Neurosci. 8:1988;2123-2130.
8. Chen Q.Y., Rowley M.J., Byrne G.C., Jones T.W., Tuomi T., Knowles W.J., Zimmet P.Z., Mackay I.R. Antibodies to glutamic acid decarboxylase in Australian children with insulin-dependent diabetes mellitus and their first-degree relatives. Pediatr. Res. 34:1993;785-790.
9. Daw K., Powers A.C. Two distinct glutamic acid decarboxylase auto-antibody specificities in IDDM target different epitopes. Diabetes. 44:1995;216-220.
10. Elble R. A simple and efficient procedure for transformation of yeasts. Biotechniques. 13:1992;18-20.
11. Erlander M.G., Tillakaratne N.J., Feldblum S., Patel N., Tobin A.J. Two genes encode distinct glutamate decarboxylases. Neuron. 7:1991;91-100.
12. Gietz R.D., Sugino A. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. 74:1988;527-534.
13. European Prediabetes Study Group, Guazzarotti L., Thivolet C., Tardivel I., Chevalier A., Carel J. Reactivity of Islet Cell Antibodies (ICA) to Recombinant Glutamic Acid Decarboxylase (GAD). J. Autoimmun. 8:1995;901-914.
14. r isoform of glutamic acid decarboxylase. Diabetes. 42:1993;631-636.
15. Kaufman D.L., Erlander M.G., Clare-Salzler M., Atkinson M.A., Maclaren N.K., Tobin A.J. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. J. Clin. Invest. 89:1992;283-292.
16. Matsuba T., Yano M., Abiru N., Takino H., Akazawa S., Nagataki S., Yasukawa K. Expression of recombinant human glutamic acid decarboxylase (GAD) in myeloma cells and enzyme-linked immunosorbent assay (ELISA) for autoantibodies to GAD. J. Biochem. 121:1997;20-24.
17. Moody A.J., Hejnæs K.R., Marshall M.O., Larsen F.S., Boel E., Svendsen I., Mortensen E., Dyrberg T. Isolation by anion-exchange of immunologically and enzymatically active human islet glutamic acid decarboxylase 65 over expressed in Sf9 insect cells. Diabetologia. 38:1995;14-23.
18. Myers M.A., Mackay I.R., Zimmet P.Z., Rowley M.J. Autoantibodies to glutamic acid decarboxylase in insulin dependent diabetes mellitus. Diabetes Annu. 10:1996;15-36.
19. Papouchado M.L., Valdez S.N., Ghiringhelli D., Poskus E., Ermacora M.R. Expression of properly folded human glutamate decarboxylase 65 as a fusion protein in Escherichia coli. Eur. J. Biochem. 264:1997;350-359.
20. 125I-labelled recombinant GAD65 produced in yeast. Clin. Chim. Acta. 256:1996;175-188.
21. Richter W., Shi Y., Baekkeskov S. Autoreactive epitopes defined by diabetes-associated human monoclonal antibodies are localized in the middle and C-terminal domains of the smaller form of glutamate decarboxylase. Proc. Natl. Acad. Sci. U.S.A. 90:1993;2832-2836.
22. Rowley M.J., Chen Q.-Y., Teoh K.-L., Zimmet P.Z., Tuomi T., Knowles W.J., Mackay I.R. Reactivity of glutamic acid decarboxylase with diabetes sera requires a dimeric-oligomeric form of the molecule. Clin. Exp. Immunol. 106:1996;323-328.
23. Rowley M.J., Mackay I.R., Chen Q.Y., Knowles W.J., Zimmet P.Z. Antibodies to glutamic acid decarboxylase discriminate major types of diabetes mellitus. Diabetes. 41:1992;548-551.
24. Spink D.C., Porter T.G., Wu S.J., Martin D.L. Characterization of three kinetically distinct forms of glutamate decarboxylase from pig brain. Biochem. J. 231:1985;695-703.
25. Teoh, K.-L., Fida, S., Rowley, M.J., Mackay, I.R., 1998. Autoantigenic reactivity of diabetes sera with a hybrid glutamic acid decarboxylase GAD67-65 molecule GAD67(1-101)/GAD65(96-585). Autoimmunity (in press).
26. Tuomi T., Rowley M.J., Knowles W.J., Chen Q.Y., McAnally T., Zimmet P.Z., Mackay I.R. Autoantigenic properties of native and denatured glutamic acid decarboxylase: evidence for a conformational epitope. Clin. Immunol. Immunopathol. 71:1994;53-59.
27. Tuomilehto J., Zimmet P., Mackay I.R., Koskela P., Vidgren G., Toivanen L., Tuomilehto-Wolf E., Kohtamaki K., Stengard J., Rowley M.J. Antibodies to glutamic acid decarboxylase as predictors of insulin-dependent diabetes mellitus before clinical onset of disease. Lancet. 343:1994;1383-1385.
28. Velloso L.A., Kämpe O., Hallberg A., Christmanson L., Betsholtz C., Karlsson F.A. Demonstration of GAD-65 as the main immunogenic isoform of glutamate decarboxylase in type I diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression. J. Clin. Invest. 91:1993;2084-2090.