Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris

Enzyme and Microbial Technology

Volumn 26, Issue 9-10, 2000, Pages 645-652

  Papakonstantinou, T ^a   Law, R H P ^a   Gardiner, P ^a   Rowley, M J ^a   Mackay, I R ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Glutamic acid decarboxylase (GAD); Hexa Histidine tag; Type 1 diabetes; Yeast

Indexed keywords

AMINO ACIDS; ANTIBODIES; ANTIGENS; BIOASSAY; CELLS; DISEASES; ENZYME KINETICS; IMMUNOLOGY; PURIFICATION; YEAST;

ALCOHOL OXIDASE PROMOTER; DIABETES; GLUTAMIC ACID DECARBOXYLASE; HISTIDINE; PHOSPHOGLYCERATE KINASE; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

ENZYMES;

GLUTAMATE DECARBOXYLASE;

CELL TRANSFORMATION; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME PURIFICATION; IMMUNOREACTIVITY; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; RADIOIMMUNOPRECIPITATION; SACCHAROMYCES CEREVISIAE; YEAST;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 0034212493     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00154-X     Document Type: Article

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