Interference of some aqueous two-phase system phase-forming components in protein determination by the Bradford method

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 719-724

  Silvério, Sara C ^a,b   Moreira, Sérgio ^c   Milagres, Adriane M F ^c   Macedo, Eugénia A ^b   Teixeira, José A ^a   Mussatto, Solange I ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Aqueous two phase system; Bradford; Interference; Polymers; Salts

Indexed keywords

CALIBRATION; MAGNESIUM COMPOUNDS; MAMMALS; NITROGEN COMPOUNDS; PHOSPHATES; POLYMERS; PROTEINS; SALTS; SODIUM BICARBONATE; SODIUM SULFATE; SOLVENT EXTRACTION; WAVE INTERFERENCE;

AQUEOUS TWO PHASE SYSTEM; BOVINE SERUM ALBUMINS; BRADFORD; MAXIMUM CONCENTRATIONS; PROTEIN DETERMINATION; PROTEIN QUANTIFICATION; SODIUM PHOSPHATE BUFFER; SODIUM-ACETATE BUFFER;

POTASSIUM COMPOUNDS;

ACETIC ACID; AMMONIUM SULFATE; BICARBONATE; BOVINE SERUM ALBUMIN; CITRIC ACID; DIPOTASSIUM HYDROGEN PHOSPHATE; DISODIUM HYDROGEN PHOSPHATE; FICOLL; FORMATE SODIUM; HYDROXYPROPYL STARCH; LITHIUM SULFATE; MACROGOL 20000; MACROGOL 4000; MACROGOL 8000; MAGNESIUM SULFATE; POLYMER; POTASSIUM DIHYDROGEN PHOSPHATE; POTASSIUM FORMATE; POVIDONE; SODIUM CARBONATE; SODIUM CHLORIDE; SODIUM DIHYDROGEN PHOSPHATE; UCON 3900; UNCLASSIFIED DRUG; WATER;

AQUEOUS TWO PHASE SYSTEM; ARTICLE; BRADFORD METHOD; CALIBRATION; CHEMICAL PARAMETERS; NONHUMAN; PH; PRIORITY JOURNAL; PROCEDURES; PROTEIN ANALYSIS; PROTEIN DETERMINATION;

BOVINAE;

EID: 84859726807     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.12.020     Document Type: Article

References (19)

1. B.Y. Zaslavsky, Aqueous Two-Phase Partitioning: Physical Chemistry and Bioanalytical Applications, Marcel Dekker, New York, 1994.
2. M. Pereira, Y. Wu, A. Venâncio, J.A. Teixeira, Aqueous two-phase extraction using thermoseparating polymer: a new system for the separation of endopolygalacturonase, Biochem. Eng. J. 15 (2003) 131-138. (Pubitemid 36734021)
3. P.P. Madeira, J.A. Teixeira, E.A. Macedo, L.M. Mikheeva, B.Y. Zaslavsky, "On the Collander equation": protein partitioning in polymer/polymer aqueous two-phase systems, J. Chromatogr. A 1190 (2008) 39-43.
4. S. Fernandes, H. Kim, R. Hatti-Kaul, Affinity extraction of dye- and metal ionbinding proteins in polyvinylpyrrolidone-based aqueous two-phase system, Protein Expr. Purif. 24 (2002) 460-469. (Pubitemid 35420071)
5. 4aqueous two-phase system, Biochem. Eng. J. 51 (2010) 24-31.
6. B.K. Sarangi, D.P. Pattanaik, K. Rathinaraj, N.M. Sachindra, M.C. Madhusudan, N.S. Mahendrakar, Purification of alkaline protease from chicken intestine by aqueous two phase system of polyethylene glycol and sodium citrate, J. Food Sci. Technol. 48 (2011) 36-44.
7. F.C. Oliveira, J.S.R. Coimbra, L.H.M. Silva, E.E.G. Rojas, M.C.H. Silva, Ovomucoid partitioning in aqueous two-phase systems, Biochem. Eng. J. 47 (2009) 55-60.
8. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
9. H.J. Chial, H.B. Thomson, A.G. Splittgerber, A spectral study of the charge forms of Coomassie Blue G, Anal. Biochem. 209 (1993) 258-266. (Pubitemid 23130183)
10. H.J. Chial, A.G. Splittgerber, A comparison of the binding of Coomassie Brilliant Blue to proteins at low and neutral pH, Anal. Biochem. 213 (1993) 362-369. (Pubitemid 23271264)
11. S.J. Compton, C.G. Jones, Mechanism of dye response and interference in the Bradford protein assay, Anal. Biochem. 151 (1985) 369-374. (Pubitemid 16160851)
12. R.W. Congdon, G.W. Muth, A.G. Splittgerber, The binding interaction of Coomassie Blue with proteins, Anal. Biochem. 213 (1993) 407-413. (Pubitemid 23271270)
13. J.J. Sedmak, S.E. Grossberg, A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250, Anal. Biochem. 79 (1977) 544-552. (Pubitemid 8105123)
14. N. Kruger, The Protein Protocols Handbook, Part I: The Bradford Method for Protein Quantitation, second ed., Humana, Totowa, NJ, 2002.
15. S. Friedenauer, H.H. Berlet, Sensitivity and variability of the Bradford protein assay in the presence of detergents, Anal. Biochem. 178 (1989) 263-268. (Pubitemid 19125344)
16. T. Marshall, K.M. Williams, Drug interference in the Bradford and 2,2′- bicinchoninic acid protein assays, Anal. Biochem. 198 (1991) 352-354.
17. S.P. Banik, S. Pal, S. Ghorai, S. Chowdhury, S. Khowala, Interference of sugars in the Coomassie Blue G dye binding assay of proteins, Anal. Biochem. 386 (2009) 113-115.
18. N. Carlsson, A. Borde, S. Wölfel, B. Åkerman, A. Larsson, Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers, Anal. Biochem. 411 (2011) 116-121.
19. H. Barbosa, N.K.H. Slater, J.C. Marcos, Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method, Anal. Biochem. 395 (2009) 108-110.