Identification and functional analysis of truncated human glutamic acid decarboxylase 65

Journal of Biomedical Science

Volumn 10, Issue 6, 2003, Pages 617-624

  Wei, Jianning ^a,b   Jin, Ying ^b   Wu, Heng ^b   Sha, Di ^b   Wu, Jang Yen ^a,b  

^a FLORIDA ATLANTIC UNIVERSITY   (United States)

^b UNIVERSITY OF KANSAS   (United States)

Author keywords

Endogenous proteases; GABA; GABA biosynthesis; L Glutamate acid decarboxylase; Truncated L glutamic acid decarboxylase

Indexed keywords

ARGININE; BLOOD CLOTTING FACTOR 10A; BRAIN ENZYME; GLUTAMATE DECARBOXYLASE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; ISOENZYME; PROTEINASE INHIBITOR; TRYPSIN; 4 AMINOBUTYRIC ACID; ARGININE DERIVATIVE; GLUTAMATE DECARBOXYLASE 65; PROTEINASE; UNCLASSIFIED DRUG; ISOPROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CONTROLLED STUDY; HUMAN; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; SEQUENCE ANALYSIS; BIOSYNTHESIS; BRAIN; PROTEIN DEGRADATION; REGULATORY MECHANISM; BRAIN LEVEL; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM;

BRAIN; BRAIN CHEMISTRY; FACTOR XA; GLUTAMATE DECARBOXYLASE; HUMANS; MOLECULAR WEIGHT; PROTEIN ISOFORMS; RECOMBINANT FUSION PROTEINS;

EID: 0242362721     PISSN: 10217770     EISSN: 14230127     Source Type: Journal    
DOI: 10.1159/000073527     Document Type: Article

References (30)

1. Aicher B, Lerch MM, Muller T, Schilling J, Ullrich A. Cellular redistribution of protein tyrosine phosphatases LAR and PTPsigma by inducible proteolytic processing. J Cell Biol 138:681-691;1997.
2. Al-Bukhari TAMA, Radford PM, Bouras G, Davenport C, Trigwell SM, Bottazzo GF, Lai M, Schwartz HL, Tighe PJ, Todd I. Distinct antigenic features of linear epitopes at the N-terminus and C-terminus of 65 kDa glutamic acid decarboxylase (GAD65): Implications for autoantigen modification during pathogenesis. Clin Exp Immunol 130:131-139;2002.
3. Asada H, Kawamura Y, Maruyama K, Kume H, Ding R, Ji FY, Kanbara N, Kuzume H, Sanbo M, Yagi T, Obata K. Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures. Biochem Biophys Res Commun 229:891-895;1996.
4. Bu DF, Erlander MG, Hitz BC, Tillakaratne NJ, Kaufman DL, Wagner-McPherson CB, Evans GA, Tobin AJ. Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Proc Natl Acad Sci USA 89:2115-2119;1992.
5. Buss K, Drewke C, Lohmann S, Piwonska A, Leistner E. Properties and interaction of heterologously expressed glutamate decarboxylase isoenzymes GAD(65kDa) and GAD(67kDa) from human brain with ginkgotoxin and its 5′-phosphate. J Med Chem 44:3166-3174;2001.
6. Chang Y-C, Gottlieb DI. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J Neurosci 8:2123-2130;1988.
7. Chattopadhyay S, Ito M, Cooper JD, Brooks AI, Curran TM, Powers JM, Pearce DA. An autoantibody inhibitory to glutamic acid decarboxylase in the neurodegenerative disorder Batten disease. Hum Mol Genet 11:1421-1431;2002.
8. 2-terminal domain. J Cell Biol 118:309-320;1992.
9. Davis KM, Foos T, Bates CS, Tucker E, Hsu CC, Chen W, Jin H, Tyburski JB, Schloss JV, Tobin AJ, Wu JY. A novel method for expression and large-scale production of human brain L-glutamate decarboxylase. Biochem Biophys Res Commun 267:777-782;2000.
10. Dinkel K, Meinck HM, Jury KM, Karges W, Ritcher W. Inhibition of γ-aminobutyric acid synthesis by glutamic acid decarboxylase autoantibodies in stiff man syndrome. Ann Neurol 44:194-201;1998.
11. Ehlers MD. Activity level controls postsynaptic composition and signaling via the ubiquitin-protease system. Nat Neurosci 6:231-242;2003.
12. Gu M, Majerus PW. The properties of the protein tyrosine phosphatase PTPMEG. J Biol Chem 271:27751-27759;1996.
13. Hampe CS, Lundgren P, Daniels TL, Hammerle LP, Marcovina SM, Lernmark Å. A novel monoclonal antibody specific for the N-terminal end of GAD65. J Neuroimmunol 113:63-71;2001.
14. Hsu CC, Davis KM, Jin H, Foos T, Floor E, Chen W, Tyburski JB, Yang CY, Schloss JV, Wu JY. Association of L-glutamic acid decarboxylase to the 70-kDa heat shock protein as a potential anchoring mechanism to synaptic vesicles. J Biol Chem 275:20822-20828;2000.
15. Jin H, Wu H, Osterhaus G, Wei J, Davis K, Sha D, Floor E, Hsu CC, Kopke RD, Wu JY. Demonstration of functional coupling between gamma-aminobutyric acid (GABA) synthesis and vesicular GABA transport into synaptic vesicles. Proc Natl Acad Sci USA 100:4293-4298;2003.
16. Kanaani J, El-Husseini AE, Aguilera-Moreno A, Diacovo JM, Bredt DS, Baekkeskov S. A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65. J Cell Biol 158:1229-1238;2002.
17. Kash SF, Johnson RS, Tecott LH, Noebels JL, Mayfoeld RD, Hanahan D, Baekkeskov S. Epilepsy in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci USA 94:14060-14065;1997.
18. Kash SF, Tecott LH, Hodge C, Baekkeskov S. Increased anxiety and altered response to anxiolytics in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci USA 96:1698-1703;1999.
19. 2-terminal epitope in the autoantigen GAD65, distinguish Stiff-Man syndrome from insulin-dependent diabetes mellitus. J Exp Med 180:595-606;1994.
20. Legay F, Henry S, Tappaz M. Evidence for two distinct forms of native glutamic acid decarboxylase in rat brain soluble extract: An immunobloting study. J Neurochem 48:1022-1026;1987.
21. Monard D. Cell-derived proteases and protease inhibitors as regulators of neurite outgrowth. Trends Neurosci 11:541-544;1988.
22. Nakagami Y, Abe K, Nishuyama N, Matsuki N. Laminin degradation by plasmin regulates long-term potentiation. J Neurosci 20:2003-2010;2000.
23. Nguyen T-H, Paul S, Xu YH, Gurd JW, Lombroso PJ. Calcium-dependent cleavage of striatal enriched tyrosine phosphatase (STEP). J Neurochem 73:1995-2001;1999.
24. Rock MT, Brooks WH, Roszman TL. Calcium-dependent signaling pathways in T cells: Potential role of calpain, protein tyrosine phosphatase 1B, and p130Cas in integrin-mediated signaling events. J Biol Chem 272:33377-33383;1997.
25. Sheikh SN, Martin DL. Heteromers of glutamate decarboxylase isoforms occur in rat cerebellum. J Neurochem 66:2082-2090;1996.
26. Shi YG, Veit B, Baekkeskov S. Amino acid residues 24-31 but not palmitoylation of cysteines 30 and 45 are required for membrane anchoring of glutamic acid becarboxylase, GAD65. J Cell Biol 124:927-934;1994.
27. Solimena M, Folli F, Aparisi R, Pozza G, De Camilli P. Autoantibodies to GABAergic neurons and pancreatic β cells in stiff man syndrome. N Engl J Med 322:1555-1560;1990.
28. Wu JY, Denner LA, Wei SC, Lin, CT, Song GX, Xu YF, Liu JW, Lin HS. Production and characterization of polyclonal and monoclonal antibodies to rat brain L-glutamate decarboxylase. Brain Res 373:1-14;1986.
29. Wu JY, Mastsuda T, Roberts E. Purification and characterization of glutamate decarboxylase from mouse brain. J Biol Chem 248:3029-3034;1973.
30. Yuan JY, Yankner BA. Apoptosis in the nervous system. Nature 407:802-809;2000.