New protamine quantification method in microtiter plates using o-phthaldialdehyde/N-acetyl-L-cysteine reagent

International Journal of Pharmaceutics

Volumn 283, Issue 1-2, 2004, Pages 11-17

  Lochmann, Dirk ^a   Stadlhofer, Sylvia ^b   Weyermann, Jörg ^a   Zimmer, Andreas ^b  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY OF GRAZ   (Austria)

Author keywords

Microtiter plate; N acetyl L cysteine; OPA NAC reagent; Ortho phthaldialdehyde; Protamine; Quantification

Indexed keywords

2 PHTHALDIALDEHYDE; ACETYLCYSTEINE; DNA; EXCIPIENT; ISOINDOLE DERIVATIVE; MERCAPTOETHANOL; NANOPARTICLE; PROTAMINE; PROTAMINE CHLORIDE; PROTAMINE SULFATE; REAGENT; UNCLASSIFIED DRUG;

AQUEOUS SOLUTION; ARTICLE; EXCITATION; FLUORESCENCE; MICROTITER PLATE ASSAY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ACETYLCYSTEINE; HEPARIN ANTAGONISTS; INDICATORS AND REAGENTS; O-PHTHALALDEHYDE; PROTAMINES; TECHNOLOGY, PHARMACEUTICAL;

EID: 4444354996     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2004.05.032     Document Type: Article

References (30)

1. Albin M., Weinberger R., Sapp E., Moring S. Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques. Anal. Chem. 63:1991;417-422
2. Ando, Yamasaki, Suzuki, 1973. Protamines: Isolation, Characterisation, Structure and Function. Springer Verlag, Berlin.
3. Boulikas T., Martin F. Histones, protamine, and polylysine but not poly(E:K) enhance transfection efficiency. Int. J. Onc. 10:1997;317-322
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
5. Campins-Falcó P., Molins-Legua C., Sevillano-Cabeza A., Tortaja Genaro L.A. o-Phthalaldehyde-N-acetylcystein polyamine derivates: formation and stability in solution and C18 support. J. Chrom. B. 759:2001;285-297
6. Chan J.K., Thompson J.W., Gill T.A. Quantitative determination of protamines by coomassie blue G assay. Anal. Biochem. 226:1995;191-193
7. Chen B.M., Xia L.W., Zhao R.Q. Determination of N(G)N(G)-dimethylarginine in human plasma by high- performance liquid chromatography. J. Chromatogr. B: Biomed. Sci. Appl. 692:1997;467-471
8. Chen B.M., Xia L.W., Liang S., Chen G., Deng F., Zhang W., Tao L. Simultaneous determination of L-arginine and dimethylarginines in human urine by high-performance liquid chromatography. Analytica Chimica Acta. 444:2001;223-227
9. Conca R., Bruzzoniti M., Mentasti E., Sarzanini C., Hajos P. Ion chromatographic separation of polyamines: putrescine, spermidine and spermine. Analytica Chimica Acta. 439:2001;107-114
10. Gonzalez Ferreiro M., Tillman L., Hardee G., Bodmeier R. Characterization of complexes of an antisense oligonucleotide with protamine and poly-L-lysine salts. J. Control Release. 73:2001;381-390
11. Junghans M., Kreuter J., Zimmer A. Antisense delivery using protamine-oligonucleotide particles. Nucl. Acids Res. 28:2000;e45
12. Junghans M., Kreuter J., Zimmer A. Phosphodiester and phosphorothioate oligonucleotide condensation and preparation of antisense nanoparticles. Biochim. Biophys. Acta. 1544:2001;177-188
13. Kang H.J., Stanley E.H., Park S.S. A sensitive method for the measurement of ammonium in soil extract and water. Com. Soil Sci. Plant Anal. 34:2003;2193-2201
14. Kutlan D., Presits P., Molnar-Perl I. Behavior and characteristics of amines derivatives obtained with o-phthaldialdehyde/3-mercaptopropionic acid and with o-phthaldialdehyde/N-acetyl-L-cysteine reagents. J. Chromatogr. A. 949:2002;235-248
15. Lochmann H., Bazzanella A., Bächmann K. Analysis of solutes and metabolites in single plant cell vacuoles by capillary electrophoresis. J. Chromatogr. A. 817:1998;337-343
16. Lochmann, D., Vogel, V., Weyermann, J., Dinauer, N., von Briesen, H., Kreuter, J., Schubert, D., Zimmer, A., 2004. Physicochemical characterisation of protamine-phosphorothioate nanoparticles. J. Microen Capsulation (in press).
17. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275
18. Mengerink Y., Kutlan D., Toth F., Csampai A., Molnar-Perl I. Advances in the evaluation of the stability and characteristics of the amino aci amd amine derivatives obtained with the o-phthaldialehyde/3-mercaptopropionic acid and o-phthaldialdehye/N-acetyl-L-cysteine reagents High-performance liquie chromatography-mass spectroscopy study. J. Chromatogr. A. 949:2002;99-124
19. Moliner Martinez Y., Molins Legua C., Campins Falcó P. Analysis of primary aliphatic short-chain monoamines by LC in water samples. Talanta. 62:2004;373-382
20. Molnar-Perl I. Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives. J. Chromatogr. A. 913:2001;283-302
21. Molnar-Perl I., Bozor I. Comparison of the stability and UV and fluorescence characteristics of the o-phthaldialdehyd/3-mercaptopropionic acid and o-phthaldialdehyd/N-acetyl-L-cysteine reagents and those of their amino acid derivatives. J. Chromatogr. A. 798:1998;37-46
22. Okamoto Y., Ogawa K., Motohiro T., Nishi N., Muta E., Ota S. Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). J. Biochem. (Tokyo). 113:1993;658-664
23. Pi J., Kumagai Y., Sun G., Shimojo N. Improved method for simultaneous determination of L-arginine and its mono- and dimethylated metabolites in biological samples by high-performance liquid chromatography. J. Chromatogr. B: Biomed. Sci. Appl. 742:2000;199-203
24. Roth M. Fluorescence reaction for amino acids. Anal. Chem. 43:1971;880-882
25. Saito K., Horie M., Nakazawa H. Kinetic study of the stability of the o-phthalaldehyde-spermine fluorophore formed by on-column derivatization. Anal. Chem. 66:1994;134-138
26. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goehe N.M., Olson B.J., Klenk D.C. Measurements of protein using bicinchoninic acid. Anal. Biochem. 150:1985;76-85
27. Sorgi F.L., Bhattacharya S., Huang L. Protamine sulfate enhances lipid-mediated gene transfer. Gene Therapy. 4:1997;961-968
28. Venza M., Visalli M., Cicciu D., Teti D. Determination of polyamines in human saliva by high-performance liquid chromatography with fluorescence detection. J. Chromatogr. B: Biomed. Sci. Appl. 757:2001;111-117
29. Weber C.J. A modification of Sakaguchi reaction for the quantitative determination of arginine. J. Biol. Chem. 86:1929;217-222
30. Wehling K., Krauss S., Wagner K.G. Cooperative binding of fluorescein-labeled clupeine by DNA. Nucl. Acids Res. 3:1976;149-164