Design of protease-resistant peptide ligands for the purification of antibodies from human plasma

Journal of Chromatography A

Volumn 1445, Issue , 2016, Pages 93-104

  Menegatti, Stefano ^a,b   Bobay, Benjamin G ^b   Ward, Kevin L ^a   Islam, Tuhidul ^a   Kish, William S ^a   Naik, Amith D ^b   Carbonell, Ruben G ^a,b  

^a North Carolina State University   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Affinity chromatography; Molecular docking; Peptide affinity ligands; Polyclonal antibodies; Protease; Proteolysis

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BINDING ENERGY; BINDING SITES; BINS; CHROMATOGRAPHY; DIGITAL LIBRARIES; FREE ENERGY; LIGANDS; PEPTIDES; PLASMA (HUMAN); PROTEOLYSIS; PURIFICATION;

AFFINITY LIGANDS; CHROMATOGRAPHIC RESINS; FREE ENERGY OF BINDING; MOLECULAR DOCKING; NON NATURAL AMINO ACIDS; POLYCLONAL ANTIBODY; PROTEASE; PROTEOLYTIC RESISTANCE;

ANTIBODIES;

ANTIBODY; CHYMOTRYPSIN A; IMMUNOGLOBULIN FC FRAGMENT; LIGAND; POLYCLONAL ANTIBODY; PROTEIN VARIANT; PROTEINASE; SYNTHETIC PEPTIDE; TRYPSIN; ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN; PEPTIDE; PROTEIN BINDING;

ADSORPTION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BINDING SITE; BIOSEPARATION; CHROMATOGRAPHY; CONTROLLED STUDY; DISSOCIATION CONSTANT; MOLECULAR DOCKING; MOLECULAR MODEL; NONHUMAN; PLASMA; PRIORITY JOURNAL; PROTEIN DEGRADATION; PURIFICATION; AFFINITY CHROMATOGRAPHY; ANIMAL; BLOOD ANALYSIS; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

ANIMALS; ANTIBODIES; BINDING SITES; BLOOD CHEMICAL ANALYSIS; CHROMATOGRAPHY, AFFINITY; CHYMOTRYPSIN; HUMANS; LIGANDS; PEPTIDES; PROTEIN BINDING; TRYPSIN;

EID: 84962861444     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2016.03.087     Document Type: Article

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