Hybridoma technology for the generation of monoclonal antibodies

Methods in Molecular Biology

Volumn 901, Issue , 2012, Pages 117-135

  Zhang, Chonghui ^a  

^a NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Cell fusion; ELISA; Flow cytometry; Hybridoma technology; Immunization; Immunohistochemistry; Monoclonal antibody; Myeloma cells; Screening; Therapeutic antibody

Indexed keywords

MONOCLONAL ANTIBODY;

ANIMAL; CELL FUSION; CYTOLOGY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HYBRIDOMA; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METABOLISM; REVIEW;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL FUSION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; HYBRIDOMAS; IMMUNOHISTOCHEMISTRY;

MUS;

EID: 84864119483     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-931-0_7     Document Type: Review

References (42)

1. Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495-497
2. Little M, Kipriyanov SM, Gall FL, Moldenhauer G (2000) Of mice and men: Hybridoma and recombinant antibodies. Immunol Today 21: 364-370
3. An Z (2010) Monoclonal antibodies-a proven and rapidly expanding therapeutic modality for human diseases. Protein Cell 1:319-330
4. Weiner LM (2007) Building better magic bullets-improving unconjugated monoclonal antibody therapy for cancer. Nat Rev Cancer 7: 701-706
5. Schlossman SF et al (1995) Leucocyte typing V: White cell differentiation antigens. Oxford University Press, Oxford
6. Matesanz-Isabel J, Sintes J, Llinàs L et al (2011) New B-cell CD molecules. Immunol Lett 134:104-112
7. Kung P, Goldstein G, Reinherz EL, Schlossman SF (1979) Monoclonal antibodies defining distinctive human T cell surface antigens. Science 206:347-349
8. Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci U S A 76:4061-4065
9. Meuer SC, Hussey RE, Hodgdon JC et al (1982) Surface structures involved in target recognition by human cytotoxic T lymphocytes. Science 218:471-473
10. Zhang C, Ao Z, Seth A, Schlossman SF (1996) A mitochondrial membrane protein defined by a novel monoclonal antibody is preferentially detected in apoptotic cells. J Immunol 157: 3980-3987
11. Zhang C (1998) Monoclonal antibody as a probe for characterization and separation of apoptotic cells. In: Zhu L, Chun J (eds) Apoptosis detection and assay methods. BioTechniques series on molecular laboratory methods. BioTechniques Books, Eaton Publishing, Natick, MA, pp 63-73
12. Bok RA, Small EJ (2002) Bloodborne biomolecular markers in prostate cancer development and progression. Nat Rev Cancer 2:918-926
13. Wagner PD, Maruvada P, Srivastava S (2004) Molecular diagnostics: A new frontier in cancer prevention. Exp Rev Mol Diagn 4:503-511
14. Jones PT, Dear PH, Foote J, Neuberger MS, Winter G (1986) Replacing the complementaritydetermining regions in a human antibody with those from a mouse. Nature 321:522-525
15. Riechmann L, Clark M, Waldmann H, Winter G (1988) Reshaping human antibodies for therapy. Nature 332:323-327
16. Smith SL (1996) Ten years of Orthoclone OKT3 (muromonab-CD3): A review. J Transpl Coord 6:109-119
17. Lupo L, Panzera P, Tandoi F et al (2008) Basiliximab versus steroids in double therapy immunosuppression in liver transplantation a prospective randomized clinical trial. Transplantation 86:925-931
18. Van Den Brande JM, Braat H, Van Den Brink GR et al (2003) Infliximab but not etanercept induces apoptosis in lamina propria T-lymphocytes from patients with Crohn's disease. Gastroenterology 124:1774-1785
19. Chan AC, Carter PJ (2010) Therapeutic antibodies for autoimmunity and inflammation. Nat Rev Immunol 10:301-316
20. Maloney DG, Grillo-López AJ, White CA et al (1997) IDEC-C2B8 (Rituximab) anti-CD20 monoclonal antibody therapy in patients with relapsed low-grade non-Hodgkin's lymphoma. Blood 90:2188-2195
21. Meissner HC, Welliver RC, Chartrand SA et al (1999) Immunoprophylaxis with palivizumab, a humanized respiratory syncytial virus monoclonal antibody, for prevention of respiratory syncytial virus infection in high risk infants: A consensus opinion. Pediatr Infect Dis J 18:223-231
22. Blattman JN, Greenberg PD (2004) Cancer immunotherapy: A treatment for the masses. Science 305:200-205
23. Reichert JM (2011) Antibody-based therapeutics to watch in 2011. mAbs 3:76-99
24. McCafferty J, Griffiths AD, Winter G, Chiswell DJ (1990) Phage antibodies: Filamentous phage displaying antibody variable domains. Nature 348:552-554
25. Hoogenboom HR (2005) Selecting and screening recombinant antibody libraries. Nat Biotechnol 23:1105-1116
26. Traggiai E, Becker S, Subbarao K et al (2004) An Efficient method to make human monoclonal antibodies from memory B cells: Potent neutralization of SARS coronavirus. Nat Med 10:871-875
27. Lanzavecchia A, Bernasconi N, Traggiai E et al (2006) Understanding and making use of human memory B cells. Immunol Rev 211: 303-309
28. Nossal GJV (1992) The molecular and cellular basis of affinity maturation in the antibody response. Cell 68:1-2
29. Jakobovits A, Amado RG, Yang X, Roskos L, Schwab G (2007) From XenoMouse technology to panitumumab, the first fully human antibody product from transgenic mice. Nat Biotechnol 25:1134-1143
30. Brüggemann M, Neuberger MS (1996) Strategies for expressing human antibody repertoires in transgenic mice. Immunol Today 17: 391-397
31. Green LL (1999) Antibody engineering via genetic engineering of the mouse: XenoMouse strains are a vehicle for the facile generation of therapeutic human monoclonal antibodies. J Immunol Methods 231:11-23
32. Lonberg N (2005) Human antibodies from transgenic animals. Nat Biotechnol 23: 1117-1125
33. Davis CG, Gallo ML, Corvalan JRF (1999) Transgenic mice as a source of fully human antibodies for the treatment of cancer. Cancer Metastasis Rev 18:421-425
34. Zhang C, Xu Y, Gu J, Schlossman SF (1998) A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis. Proc Natl Acad Sci U S A 95:6290-6295
35. Yonehara S, Ishii A, Yonehara M (1989) A cellkilling monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor. J Exp Med 169:1747-1756
36. Trauth BC, Klas C, Peters AMJ et al (1989) Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science 245:301-305
37. Kearney JF, Radbruch A, Liesegang B, Rajewsky K (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol 123:1548-1550
38. Shi S-R, Shi Y, Taylor CR (2011) Antigen retrieval immunohistochemistry: Review and future prospects in research and diagnosis over two decades. J Histochem Cytochem 59:13-32
39. D'Amico F, Skarmoutsou E, Stivala F (2009) State of the art in antigen retrieval for immunohistochemistry. J Immunol Methods 341:1-18
40. Glassy M (1988) Creating hybridomas by electrofusion. Nature 333:579-580
41. Ohnishi K, Chiba J, Goto Y, Tokunaga T (1987) Improvement in the basic technology of electrofusion for generation of antibodyproducing hybridomas. J Immunol Methods 100:181-189
42. Long WL, McGuire W, Palombo A, Emini EA (1986) Enhancing the establishment efficiency of hybridoma cells: Use of irradiated human diploid fi broblast feeder layers. J Immunol Methods 86:89-93