Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin

Journal of Chromatography A

Volumn 1260, Issue , 2012, Pages 61-66

  Naik, Amith D ^a   Menegatti, Stefano ^a   Reese, Hannah R ^a   Gurgel, Patrick V ^a   Carbonell, Ruben G ^a  

^a North Carolina State University   (United States)

Author keywords

Dextran coated charcoal; Hexapeptide ligand; IgG purification; Lemna minor; Monoclonal antibody; Phenolics; Transgenic plants

Indexed keywords

AFFINITY LIGANDS; AFFINITY RESINS; AFFINITY SEPARATION; ANTIBODY CONCENTRATION; HEXAMERS; HEXAPEPTIDE LIGANDS; HUMAN PATHOGENS; LEMNA MINOR; MAMMALIAN CELL CULTURE; PHENOLIC COMPOUNDS; PHENOLICS; POLYMETHACRYLATES; PRE-TREATMENT; PRODUCTION COST; PROTEIN A; SUPERNATANTS; THERAPEUTIC PROTEIN; TRANSGENIC PLANTS; TRANSGENICS;

CELL CULTURE; CHARCOAL; DEXTRAN; LIGANDS; MONOCLONAL ANTIBODIES; PEPTIDES; PLANT EXTRACTS; PURIFICATION; RESINS;

PHENOLS;

ACTIVATED CARBON; CHARCOAL; DEXTRAN CHARCOAL; LEMMA MINOR EXTRACT; LIGAND; MONOCLONAL ANTIBODY; PHENOL DERIVATIVE; PIGMENT; PLANT EXTRACT; POLYMETHACRYLIC ACID; RESIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG PURIFICATION; LEMMA MINOR; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEPARATION TECHNIQUE; TRANSGENIC PLANT;

ANTIBODIES, MONOCLONAL; ARACEAE; CHARCOAL; CHROMATOGRAPHY, AFFINITY; DEXTRANS; HUMANS; IMMUNOGLOBULIN G; PEPTIDES; PLANT EXTRACTS; PLANTS, GENETICALLY MODIFIED; PROTEIN BINDING; STAPHYLOCOCCAL PROTEIN A;

LEMNA; LEMNA MINOR; MAMMALIA;

EID: 84866529925     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.08.043     Document Type: Article

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