Affinity purification of IgG monoclonal antibodies using the D-PAM synthetic ligand: chromatographic comparison with protein A and thermodynamic investigation of the D-PAM/IgG interaction

Journal of Immunological Methods

Volumn 333, Issue 1-2, 2008, Pages 126-138

  D'Agostino, Biagio ^a   Bellofiore, Piero ^a   De Martino, Tiziana ^a   Punzo, Carmine ^a   Rivieccio, Vincenzo ^a   Verdoliva, Antonio ^a  

^a Tecnogen SpA   (Italy)

Author keywords

Affinity purification; DNA; Host cell proteins; IgG; Monoclonal antibody; Synthetic peptide ligand

Indexed keywords

D PAM PEPTIDE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY ST2146; MONOCLONAL ANTIBODY ST2485; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; THERMODYNAMICS;

ANTIBODIES, MONOCLONAL; BIOMIMETIC MATERIALS; CALORIMETRY, INDIRECT; CHROMATOGRAPHY, AFFINITY; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN G; LIGANDS; PEPTIDES; STAPHYLOCOCCAL PROTEIN A; THERMODYNAMICS;

EID: 41349108454     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.01.014     Document Type: Article

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