Quantification of oxysterols in human plasma and red blood cells by liquid chromatography high-resolution tandem mass spectrometry

Journal of Chromatography A

Volumn 1439, Issue , 2016, Pages 82-88

  Pataj, Zoltán ^a   Liebisch, Gerhard ^a   Schmitz, Gerd ^a   Matysik, Silke ^a  

^a UNIVERSITY HOSPITAL REGENSBURG   (Germany)

Author keywords

HR MS (high resolution mass spectrometry); Human plasma; Niemann Pick type C; Orbitrap; Oxysterols; Red blood cells

Indexed keywords

BLOOD; CHROMATOGRAPHY; CYTOLOGY; DRUG PRODUCTS; ELECTROSPRAY IONIZATION; IONIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PLASMA (HUMAN); SPECTROMETRY;

HIGH RESOLUTION MASS SPECTROMETRY; HUMAN PLASMAS; NIEMANN-PICK TYPE C; ORBITRAP; OXYSTEROLS; RED BLOOD CELL;

CELLS;

24 HYDROXYCHOLESTEROL; 25 HYDROXYCHOLESTEROL; 26 HYDROXYCHOLESTEROL; 4 BETA HYDROXYCHOLESTEROL; 7 OXOCHOLESTEROL; 7ALPHA HYDROXYCHOLESTEROL; 7BETA HYDROXYCHOLESTEROL; CHOLESTAN 3BETA,5ALPHA,6BETA TRIOL; DIMETHYLGLYCINE; OXYSTEROL; UNCLASSIFIED DRUG; 25-HYDROXYCHOLESTEROL; 27-HYDROXYCHOLESTEROL; 7-KETOCHOLESTEROL; BIOLOGICAL MARKER; CHOLEST-5-EN-3 BETA,7 ALPHA-DIOL; CHOLEST-5-ENE-3,4-DIOL; CHOLESTEROL DERIVATIVE;

ACCURACY; ARTICLE; BLOOD ANALYSIS; CEREBROTENDINOUS XANTHOMATOSIS; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ERYTHROCYTE; EXTRACTION; FOOD AND DRUG ADMINISTRATION; HUMAN; HUMAN CELL; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; NORMAL HUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RELIABILITY; SAPONIFICATION; TANDEM MASS SPECTROMETRY; VALIDATION PROCESS; BLOOD; CHEMISTRY; NIEMANN PICK DISEASE; PLASMA; PROCEDURES; STEREOISOMERISM;

BIOMARKERS; CHROMATOGRAPHY, LIQUID; ERYTHROCYTES; HUMANS; HYDROXYCHOLESTEROLS; KETOCHOLESTEROLS; NIEMANN-PICK DISEASE, TYPE C; PLASMA; STEREOISOMERISM; TANDEM MASS SPECTROMETRY; XANTHOMATOSIS, CEREBROTENDINOUS;

EID: 84960377043     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.11.015     Document Type: Article

References (46)

1. Russell D.W. Fifty years of advances in bile acid synthesis and metabolism. J. Lipid Res. 2009, 50:S120-S125.
2. Brown M.S., Goldstein J.L. Cholesterol feedback: from Schoenheimer's bottle to Scap's MELADL. J. Lipid Res. 2009, 50:1255.
3. Nes W.D. Biosynthesis of cholesterol and other sterols. Chem. Rev. 2011, 111:6423-6451.
4. Griffiths W.J., Crick P.J., Wang Y.Q. Methods for oxysterol analysis: past, present and future. Biochem. Pharmacol. 2013, 86:3-14.
5. Matysik S., Schmitz G. Application of gas chromatography-triple quadrupole mass spectrometry to the determination of sterol components in biological samples in consideration of the ionization mode. Biochimie 2013, 95:489-495.
6. Schroepfer G.J. Oxysterols: modulators of cholesterol metabolism and other processes. Physiol. Rev. 2000, 80:361-554.
7. Poli G., Biasi F., Leonarduzzi G. Oxysterols in the pathogenesis of major chronic diseases. Redox Biol. 2013, 1:125-130.
8. Spann N.J., Glass C.K. Sterols and oxysterols in immune cell function. Nat. Immunol. 2013, 14:893-900.
9. Bjorkhem I. Do oxysterols control cholesterol homeostasis?. J. Clin. Investig. 2002, 110:725-730.
10. Bjorkhem I., Diczfalusy U. Oxysterols - friends, foes, or just fellow passengers?. Arterioscler. Thromb. Vasc. Biol. 2002, 22:734-742.
11. Porter F.D., Scherrer D.E., Lanier M.H., Langmade S.J., Molugu V., Gale S.E., Olzeski D., Sidhu R., Dietzen D.J., Fu R., Wassif C.A., Yanjanin N.M., Marso S.P., House J., Vite C., Schaffer J.E., Ory D.S. Cholesterol oxidation products are sensitive and specific blood-based biomarkers for Niemann-Pick C1 disease. Sci. Transl. Med. 2010, 2.
12. Jiang X.T., Sidhu R., Porter F.D., Yanjanin N.M., Speak A.O., Vruchte D.T.T., Platt F.M., Fujiwara H., Scherrer D.E., Zhang J., Dietzen D.J., Schaffer J.E., Ory D.S. A sensitive and specific LC-MS/MS method for rapid diagnosis of Niemann-Pick C1 disease from human plasma. J. Lipid Res. 2011, 52:1435-1445.
13. Boenzi S., Deodato F., Taurisano R., Martinelli D., Verrigni D., Carrozzo R., Bertini E., Pastore A., Dionisi-Vici C., Johnson D.W. A new simple and rapid LC-ESI-MS/MS method for quantification of plasma oxysterols as dimethylaminobutyrate esters. Its successful use for the diagnosis of Niemann-Pick type C disease. Clin. Chim. Acta 2014, 437:93-100.
14. Klinke G., Rohrbach M., Giugliani R., Burda P., Baumgartner M.R., Tran C., Gautschi M., Mathis D., Hersberger M. LC-MS/MS based assay and reference intervals in children and adolescents for oxysterols elevated in Niemann-Pick diseases. Clin. Biochem. 2015, 48:596-602.
15. Leoni V., Masterman T., Mousavi F.S., Wretlind B., Wahlund L.O., Diczfalusy U., Hillert J., Bjorkhem I. Diagnostic use of cerebral and extracerebral oxysterols. Clin. Chem. Lab. Med. 2004, 42:186-191.
16. Lorbek G., Lewinska M., Rozman D. Cytochrome P450s in the synthesis of cholesterol and bile acids - from mouse models to human diseases. FEBS J. 2012, 279:1516-1533.
17. Matysik S., Klünemann H.H., Schmitz G. Gas chromatography-tandem mass spectrometry method for the simultaneous determination of oxysterols, plant sterols, and cholesterol precursors. Clin. Chem. 2012, 58:1557-1564.
18. Menendez-Carreno M., Garcia-Herreros C., Astiasaran I., Ansorena D. Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2008, 864:61-68.
19. Ahmida H.S.M., Bertucci P., Franzo L., Massoud R., Cortese C., Lala A., Federici G. Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography-mass spectrometry (GC-MS) with selective ion monitoring (SIM). J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2006, 842:43-47.
20. Acimovic J., Lovgren-Sandblom A., Monostory K., Rozman D., Golicnik M., Lutjohann D., Bjorkhem I. Combined gas chromatographic/mass spectrometric analysis of cholesterol precursors and plant sterols in cultured cells. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2009, 877:2081-2086.
21. McDonald J.G., Smith D.D., Stiles A.R., Russell D.W. A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma. J. Lipid Res. 2012, 53:1399-1409.
22. Helmschrodt C., Becker S., Schroter J., Hecht M., Aust G., Thiery J., Ceglarek U. Fast LC-MS/MS analysis of free oxysterols derived from reactive oxygen species in human plasma and carotid plaque. Clin. Chim. Acta 2013, 425:3-8.
23. Honda A., Yamashita K., Miyazaki H., Shirai M., Ikegami T., Xu G.R., Numazawa M., Hara T., Matsuzaki Y. Highly sensitive analysis of sterol profiles in human serum by LC-ESI-MS/MS. J. Lipid Res. 2008, 49:2063-2073.
24. Honda A., Miyazaki T., Ikegami T., Iwamoto J., Yamashita K., Numazawa M., Matsuzaki Y. Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS. J. Steroid Biochem. Mol. Biol. 2010, 121:556-564.
25. Griffiths W.J., Wang Y.Q., Alvelius G., Liu S., Bodin K., Sjovall J. Analysis of oxysterols by electrospray tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 2006, 17:341-362.
26. Crick P.J., Bentley T.W., Abdel-Khalik J., Matthews I., Clayton P.T., Morris A.A., Bigger B.W., Zerbinati C., Tritapepe L., Iuliano L., Wang Y.Q., Griffiths W.J. Quantitative charge-tags for sterol and oxysterol analysis. Clin. Chem. 2015, 61:400-411.
27. Karu K., Hornshaw M., Woffendin G., Bodin K., Hamberg M., Alvelius G., Sjovall J., Turton J., Wang Y.Q., Griffiths W.J. Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterols. J. Lipid Res. 2007, 48:976-987.
28. Jiang X.T., Ory D.S., Han X.L. Characterization of oxysterols by electrospray ionization tandem mass spectrometry after one-step derivatization with dimethylglycine. Rapid Commun. Mass Spectrom. 2007, 21:141-152.
29. Dzeletovic S., Breuer O., Lund E., Diczfalusy U. Determination of cholesterol oxidation products in human plasma by isotope dilution-mass spectrometry. Anal. Biochem. 1995, 225:73-80.
30. Bandaru V.V.R., Haughey N.J. Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum. BMC Neurosci. 2014, 15:9.
31. Roberg-Larsen H., Vesterdal C., Wilson S.R., Lundanes E. Underivatized oxysterols and nano LC-ESI-MS: a mismatch. Steroids 2015, 99:125-130.
32. Karun R., Christen I., Sailer A.W., Bitsch F., Zhang J. Detection of dihydroxycholesterols in human plasma using HPLC-ESI-MS/MS. Steroids 2015, 99:131-138.
33. Schott H.F., Lutjohann D. Validation of an isotope dilution gas chromatography-mass spectrometry method for combined analysis of oxysterols and oxyphytosterols in serum samples. Steroids 2015, 99:139-150.
34. Guidance for Industry Bioanalytical Method Validation 2001, U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research, Center for Veterinary Medicine.
35. Guideline on Bioanalytical Method Validation 2011, European Medicines Agency - Committee for Medicinal Products for Human Use.
36. Liebisch G., Matysik S. Accurate and reliable quantification of 25-hydroxy-vitamin D species by liquid chromatography-high-resolution tandem mass spectrometry. J. Lipid Res. 2015, 56:1234-1239.
37. Armbruster D.A., Pry T. Limit of blank, limit of detection and limit of quantitation. Clin. Biochem. Rev./Aust. Assoc. Clin. Biochem. 2008, 29(Suppl. 1):S49-S52.
38. Hay I.D., Bayer M.F., Kaplan M.M., Klee G.G., Larsen P.R., Spencer C.A., Wartofsky L. American-thyroid-association assessment of current free thyroid-hormone and thyrotropin measurements and guidelines for future clinical assays. Clin. Chem. 1991, 37:2002-2008.
39. Honda A., Yamashita K., Hara T., Ikegami T., Miyazaki T., Shirai M., Xu G.R., Numazawa M., Matsuzaki Y. Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MS. J. Lipid Res. 2009, 50:350-357.
40. Bjorkhem I., Lovgren-Sandblom A., Piehl F., Khademi M., Pettersson H., Leoni V., Olsson T., Diczfalusy U. High levels of 15-oxygenated steroids in circulation of patients with multiple sclerosis: fact or fiction?. J. Lipid Res. 2011, 52:170-174.
41. Leidl K., Liebisch G., Richter D., Schmitz G. Mass spectrometric analysis of lipid species of human circulating blood cells. Biochim. Biophys. Acta: Mol. Cell Biol. Lipids 2008, 1781:655-664.
42. Tesoriere L., Attanzio A., Allegra M., Cilla A., Gentile C., Livrea M.A. Oxysterol mixture in hypercholesterolemia-relevant proportion causes oxidative stress-dependent eryptosis. Cell. Physiol. Biochem. 2014, 34:1075-1089.
43. Klinke G., Mathis D., Hersberger M. Oxysterols as biomarkers for Niemann-Pick type C disease. Clin. Biochem. 2014, 47:772.
44. Cali J.J., Russell D.W. Characterization of human sterol 27-hydroxylase: a mitochondrial cytochrome-P-450 that catalyzes multiple oxidation reactions in bile-acid biosynthesis. J. Biol. Chem. 1991, 266:7774-7778.
45. Cali J.J., Hsieh C.L., Francke U., Russell D.W. Mutations in the bile-acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis. J. Biol. Chem. 1991, 266:7779-7783.
46. Koopman B.J., Vandermolen J.C., Wolthers B.G., Vanderpas J.B. Determination of some hydroxycholesterols in human-serum samples. J. Chromatogr.: Biomed. Appl. 1987, 416:1-13.