Lactococcus lactis is an Efficient Expression System for Mammalian Membrane Proteins Involved in Liver Detoxification, CYP3A4, and MGST1

Molecular Biotechnology

Volumn 58, Issue 4, 2016, Pages 299-310

  Bakari, Sana ^a   Lembrouk, Mehdi ^a   Sourd, Laura ^a   Ousalem, Fares ^a   André, François ^a   Orlowski, Stéphane ^a   Delaforge, Marcel ^a   Frelet Barrand, Annie ^a,b  

^a INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

^b UNIVERSITÉ DE FRANCHE COMTÉ   (France)

Author keywords

Cytochrome P450; Gateway; Lactococcus lactis; Membrane proteins; Microsomal glutathione S transferase

Indexed keywords

BIOLOGICAL MEMBRANES; CLONING; DETOXIFICATION; ESCHERICHIA COLI; GATEWAYS (COMPUTER NETWORKS); MAMMALS; MEMBRANES; RECONFIGURABLE HARDWARE; YEAST;

CYTOCHROME P450; DETOXIFICATION MECHANISM; EUKARYOTIC MEMBRANE PROTEIN; GLUTATHIONE S-TRANSFERASES; HETEROLOGOUS EXPRESSION; LACTOCOCCUS LACTIS; MEMBRANE PROTEINS; TECHNICAL DIFFICULTIES;

PROTEINS;

CYTOCHROME P450 3A4; GLUTATHIONE TRANSFERASE; MEMBRANE PROTEIN; MICROSOMAL GLUTATHIONE S TRANSFERASE 1; POLYHISTIDINE TAG; PROTEIN TAG; RECOMBINANT MICROSOMAL GLUTATHIONE S TRANSFERASE 1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; CYTOCHROME P450 3A; MICROSOMAL GLUTATHIONE S-TRANSFERASE-I;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; CONTROLLED STUDY; DETOXIFICATION; ENZYME ACTIVITY; ESCHERICHIA COLI; FUNGAL STRAIN; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; LACTOCOCCUS LACTIS; LIVER; LIVER DETOXIFICATION; LIVER MICROSOME; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; ANIMAL; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PROCEDURES; RAT;

ANIMALS; CLONING, MOLECULAR; CYTOCHROME P-450 CYP3A; GENE EXPRESSION; GLUTATHIONE TRANSFERASE; HUMANS; LACTOCOCCUS LACTIS; LIVER; MEMBRANE PROTEINS; RATS; RECOMBINANT PROTEINS;

EID: 84960111138     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-016-9928-z     Document Type: Article

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