Large-scale single guide RNA Library construction and use for CRISPR–Cas9-based genetic screens

Cold Spring Harbor Protocols

Volumn 2016, Issue 3, 2016, Pages 203-206

  Wang, Tim ^a,b,c   Lander, Eric S ^a,c,d   Sabatini, David M ^a,b,c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM; CELL PROLIFERATION; CELL SURVIVAL; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONSTRUCT; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC SCREENING; HIGH THROUGHPUT SEQUENCING; MICROARRAY ANALYSIS; MOLECULAR LIBRARY; NONHUMAN; PHENOTYPE; RNA LIBRARY; ANIMAL; CELL LINE; GENE INACTIVATION; GENE LIBRARY; GENETICS; HUMAN; MAMMAL; PROCEDURES;

RNA;

ANIMALS; CELL LINE; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; GENE LIBRARY; GENETIC TESTING; HUMANS; MAMMALS; RNA;

EID: 84959475088     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.top086892     Document Type: Article

References (12)

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